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All Journal HAYATI Journal of Biosciences MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Silvikultur Tropika (JST) Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sylva Lestari Indonesian Journal of Forestry Research
Rachmat, Henti Hendalastuti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Materials Science & Nanotechnology Social Sciences

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Optimizing DNA Extraction Methods from Leaf and Wood Tissues to Support Dipterocarp Conservation and Sustainable Forest Management Arrofaha, Nawwall; Rachmat, Henti Hendalastuti; Dwiyanti, Fifi Gus; Adinugroho, Wahyu Catur; Siregar, Iskandar Zulkarnaen; Kamal, Irsyad; Syaputra, Dhika; Salamah, Andi
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 16 No 1 (2026): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.16.1.13

Abstract

Dipterocarpaceae are economically important, contributing over 85% of Indonesia's timber exports. However, this crucial resource is increasingly threatened by illegal logging, habitat destruction, and the illegal timber trade, which jeopardize dipterocarp population. Furthermore, conservation efforts utilizing genetic and forensic techniques often encounter substantial challenges due to the complexities in DNA extraction protocol. To address this, the study aimed to enhance the efficiency of DNA extraction methodologies by comparing two methods: the modified cetyltrimethylammonium bromide (CTAB) and the Genomic DNA Mini Kit (Plant) from Geneaid Biotech Ltd. The research focused on leaf and wood samples from two species, spesifically Rubroshorea leprosula (Miq.) P.S.Ashton & J.Heck and Shorea laevis Ridl. For each of these species, five leaf and five wood samples were extracted using both methods. The quality of the DNA extraction was evaluated using electrophoresis and quantified with a Qubit fluorometer. Higher DNA concentrations were obtained with the modified CTAB method compared to the GeneAid kit for both R. leprosula and S. laevis, particularly in leaf tissue. The GeneAid kit consistently exhibited low DNA yield efficiency compared to the modified CTAB method for both species. Additionally, PCR amplification of both leaf and wood samples confirmed that the extracted DNA was suitable formolecular analyses. These findings not only contributed to laboratory applications but also served as practical tools for species identification and genetic conservation for sustainable forest management and law enforcement.
Co-Authors Adinugroho, Wahyu Catur Adzkia, Ulfa Andi Salamah Arida Susilowati Iswanto Arrofaha, Nawwall Attarik, Nuriskia Ayyasy, Yahya Elsa Widyastuti Fifi Gus Dwiyanti Henti Rosdayanti, Henti Hesti Lestari Tata, Hesti Lestari Husnul Khotimah I Wayan Susi Dharmawan Indah Novita Dewi Irsyad Kamal ISKANDAR ZULKARNAEN SIREGAR Istomo . Koichi Kamiya Kustiyarini, Nur Fadila KUSUMADEWI SRI YULITA Laswi Irmayanti, Laswi Majiidu, Muhammad Muharam, Karima Fauziah Nur Hayati PRASETYAWATI, C. ANDRIYANI Prijanto Pamoengkas Rahman, Mohamad Miftah Suharti, Sri Susila, Susila Syaputra, Dhika Wati, Ridha Windyoningrum, Ayun Yuliantoro, Isdomo