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All Journal Jurnal Biologi Universitas Andalas Jurnal Biodjati
Fusvita, Laifa
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Complementary Molecular Data for Two Species from Herbarium Bogoriense: Sphaerobolus stellatus and Trichaleurina javanica Yuliana, Meta; Maulana, Indra; Mulya, Sari; Fusvita, Laifa; Hermawan, Rudy
Jurnal Biologi Universitas Andalas Vol 12 No 1 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.12.1.42-46.2024

Abstract

A micro-mushroom namely Sphaerobolus stellatus (BO 24422) and macro-mushroom namely Trichaleurina javanica (BO 24420) were found in Landscape Arboretum of IPB University. The latest identification used morphological characteristic and molecular study of Large Subunit DNA marker respectively. According to their identification, the database of fungal identification is commonly from Internal Transcribed Spacer (ITS) marker for molecular study. But both specimens, S. stellatus BO 24422 and T. javanica BO 24420, have not the ITS sequences yet. This research aim to study the molecular data of two specimens using ITS sequences. The method of this research used the molecular identification using internal transcribed spacer (ITS) primer: ITS 4 as a forward and ITS 5 as a reverse. Two phylogenetic trees were generated by Randomized Axelarated Maximum Likelihood (RAxML) Black box. Bootstrap (BS) values ≥ 90% were displayed on the phylogenetic treebranches. The result showed that the bootstrap value for S. stellatus BO 24422  showed the good value as 96% classified as S. stellatus. Then, T. javanica BO 24420  had a better BS value with 93% classified as T. javanica. The ITS is a general marker and very useful for molecular identification. 
Morphological characteristic and Phenetic Relationship of Lysurus periphragmoides Collected from West Java Hermawan, Rudy; Fusvita, Laifa; Nugraha, Naufal Hafizh; Amelya, Mega Putri
Jurnal Biodjati Vol 6 No 1 (2021): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i1.10724

Abstract

Lysurus is one of the unique genera in Basidiomycetes. It has a stinky odor and slime on the head. The unusual-shaped makes the species in Lysurus easily to be identified. One of Lysurus had been found in West Java, Indonesia namely L. periphragmoides. The specimen was deposited into Herbarium Bogoriense with code BO 24418. This study aimed to obtain specimens and characterize the Lysurus BO 24418 using morphological characteristics, and analyze the phenetic relationship among Lysurus species. Lysurus BO 24418 has two phases (egg and mushroom). The egg phase is usually hypogeous underground. The mushroom has a head and stem. The head bears the mature spore with slime distribute malodor. The stem has a hollow and spongy texture with a yellowish color. The numerical data of morphological characters of species in Lysurus were analyzed using NTSys ver 2.1 software. Ten characters were used to build a dendrogram using Sequential Agglomerative Hierarchical Nested (SAHN) cluster analysis with Unweighted Pair Group Method with Arithmetic Mean (UPGMA). Phallus indusiatus was selected as an outgroup. The analyses showed the specimen was classified as L. periphragmoides with 100% of similarity coefficient and it was close L. gardneri with 40,4% of similarity coefficient. The characters that cluster among them are stem surface, head type, egg diameter, and spore max length
Co-Authors Amelya, Mega Putri Indra Maulana, Indra Mulya, Sari Nugraha, Naufal Hafizh Rudy Hermawan YULIANA, META