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LIGHT EXPOSURE’S EFFECTS ON INACTIVE STATE DURATION AND SLEEP LATENCY IN ZEBRAFISH (DANIO RERIO) LARVAE INSOMNIA MODEL Zamroni Afif; Mochammad Istiadjid Eddy Santoso; Husnul Khotimah; Irawan Satriotomo; Edi Widjajanto; Masruroh Rahayu; Shahdevi Nandar Kurniawan; Dheka Sapti Iskandar; Annisatul Hakimah; Syafiatul Azizah; Nurvia Andriani; Kartika Agustina
MNJ (Malang Neurology Journal) Vol. 8 No. 2 (2022): July
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2022.008.02.11

Abstract

Background: Insomnia is defined as difficulty falling or staying asleep or a sleep state that cannot restore the body's condition. The zebrafish (Danio rerio) is a vertebrate model that has been extensively studied to study sleep and neurological disorders. One of the most widely used methods to examine the effect of the light-dark cycle on the circadian system is by exposing animals and humans to pulse wave light. Objective: To see the effect of light exposure on zebrafish larvae by looking at inactive state duration and sleep latency in zebrafish (Danio rerio) larvae insomnia model. Methods: This study used four groups of zebrafish larvae i.e : (1) normal group (2) minutes of light exposure and 2 minutes off (2/2)), (3) Four minutes of light exposure and 1 minute off (4/1), and (4)  24 hours on (24/0). Observation of larval movement was carried out on 5, 6, and 7 dpf (day post fertilization). Observation time was 30 minutes before and after turned off of light exposure. Results: There were significant differences on days 5, 6, and 7 between the normal group and the three light treatment groups on inactive state duration and sleep latency in dark and light conditions with p-values (p<0.05) and (p< 0.031), but there was no significant difference among groups of light exposure 2 minutes on 2 minutes off, 4 minutes on 1 minute off, and 24 hours on. The 24-hour on treatment showed the most inactive state duration among the light treatments, while the sleep latency was found in the 24-hour treatment. Conclusion: Light treatment of 2 minutes on 2 minutes off, 4 minutes on 1 minute off, and 24 hours on can cause insomnia, but the most substantial insomnia effect is obtained from the 24-hour treatment.
Determinan Maternal Terhadap Kejadian Preeklampsia: Studi Cross-Sectional Berbasis Data Pelayanan Kesehatan Putri, Putri Kusuma Pandin; Dheka Sapti Iskandar; Zainuddin; Muhammad Azwar
Jurnal Kesehatan Marendeng Vol. 10 No. 1 (2026): JURNAL KESEHATAN MARENDENG
Publisher : LPPM STIKES Marendeng Majene

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58554/jkm.v10i1.202

Abstract

Preeklampsia merupakan komplikasi kehamilan yang berkontribusi signifikan terhadap morbiditas dan mortalitas maternal. Identifikasi faktor risiko maternal berbasis data pelayanan kesehatan penting untuk mendukung deteksi dini. Penelitian ini bertujuan menganalisis determinan maternal yang berhubungan dengan kejadian preeklampsia. Studi cross-sectional dilakukan pada 158 ibu hamil trimester kedua dan ketiga menggunakan data rekam medis dan wawancara terstruktur. Variabel independen meliputi usia maternal, paritas, indeks massa tubuh (IMT), riwayat hipertensi, riwayat preeklampsia sebelumnya, dan jumlah kunjungan antenatal care (ANC), sedangkan variabel dependen adalah diagnosis preeklampsia oleh dokter. Analisis menggunakan uji Chi-square dan regresi logistik dengan tingkat signifikansi 0,05. Prevalensi preeklampsia sebesar 26,6%. Usia risiko tinggi, IMT overweight/obesitas, riwayat hipertensi, riwayat preeklampsia, dan ANC tidak adekuat berhubungan signifikan dengan kejadian preeklampsia. Analisis multivariat menunjukkan riwayat hipertensi dan IMT berlebih sebagai prediktor dominan. Skrining berbasis faktor risiko perlu diperkuat dalam pelayanan antenatal.
Edukasi tata cara beretika pada mahasiswa tingkat 1 angkatan 2025/2026 prodi rekam medis dan informasi kesehatan Poltekkes Kemenkes Malang Muhammad Dudayev Caesar Putra; Dheka Sapti Iskandar; Nurul Uswatin; Putri Kusuma Pandin; Laifa Fusvita
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 10, No 2 (2026): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v10i2.37946

Abstract

Abstrak Etika dan pendidikan merupakan dua kata penting yang berbeda namun tidak dapat dipisahkan dalam praktiknya. Untuk dapat memahami kedua pokok ini sebagai modal awal dalam pemahaman yang benar tentang etika pendidikan harus didasarkan pada suatu pengertian yang benar tentang etika pendidikan itu sendiri. Visi dari prodi D3 rekam medis dan informasi kesehatan adalah “Menjadi Program Studi Diploma Tiga Rekam Medis dan Informasi Kesehatan yang Beradab dan Berdaya Saing Global dalam Bidang Klinikal Koding”. Oleh sebab itu pentingnya mahasiswa untuk mengetahui tata cara beretika yang baik dan benar agar dapat memenuhi visi prodi yaitu “beradab”. Oleh sebab itu penulis melakukan edukasi terkait etika terutama pada mahasiswa prodi D3 rekam medis dan informasi kesehatan sebagai betuk dari pengabdian masyarakat. Desain yang digunakan adalah quasi experimental pre and post test tanpa control group. Hasil pengabdian masyarakat dimana terdapat peningkatan pengetahuan pada mahasisawa tingkat 1 angkatan 2025/2026 prodi rekam medis dan Informasi kesehatan poltekkes kemenkes malang yang telah mendapatkan edukasi etika melalui poster dan buku pedoman. Rata-rata nilai pengetahuan sebelum edukasi etika (pre test) adalah 55.21 dan nilai rata-rata pengetahuan pasca pemeberian edukasi (post test) adalah 86.75. Keberhasilan kegaiatan pengabdian masyarakat ini dilihat dari adanya peningkatan Pengetahuan. Dimana terjadi peningkatan pengetahuan sebesar 57.13% pada mahasiswa. Dengan adanya peningkatan pengetahuan diharapkan selanjutnya mahasiswa lebih memahami tata cara etika yang baik dan benar. Kata kunci: edukasi; poster; buku pedoman Abstract Ethics and education are two important words that are different but cannot be separated in practice. To be able to understand these two points as the initial capital in a corect understand of educational ethics must be based on a correct understanding of educational ethics itself. The vision of the D3 medical records and health information study program is "To become a Diploma Three Medical Records and Health Information Study Program that is Civilized and Globally Competitive in the Field of Clinical Coding". Therefore, it is important for students to know good and correct ethical procedures in order to fulfill the study program's vision, namely "civilized". Therefore, the author conducted education related to ethics, especially for D3 medical records and health information students as a form of community service. The design used was a quasi-experiment pre and post test without a control group. The results of community service where there was an increase in knowledge among first-year students of the 2025/2026 class of the medical records and health information study program at the Malang Ministry of Health Polytechnic who had received ethics education through posters and guidebooks. The average knowledge score before the ethics education (pre-test) was 55.21, and the average knowledge score after the education (post-test) was 86.75. The success of this community service activity was seen in the increase in knowledge, which increased by 57.13% among students. With this increase in knowledge, it is hope that students will have a better understanding of proper and correct ethical procedures. Keywords: education; poster; guidebook
Kontaminasi Mikrobiologis Pada Roti: Tinjauan Literatur Tentang Keragaman Mikroorganisme Dan Potensi Risikonya Terhadap Kesehatan Laifa Fusvita; Dheka Sapti Iskandar
KOLONI Vol. 5 No. 3 (2026): SEPTEMBER 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/koloni.v5i3.1475

Abstract

Bread is one of the most widely consumed wheat flour-based processed foods, however, it is highly susceptible to microbiological contamination because its carbohydrate content, water content, and moisture level support microbial growth. This study aimed to review the diversity of contaminant microorganisms in bread, the sources and factors influencing contamination, potential health risks, and control measures. We conducted a literature review by searching scientific articles published between 2016 and 2026 in Google Scholar, PubMed, and ScienceDirect. The results indicate that microbiological contamination in bread involves molds (Aspergillus, Penicillium, Rhizopus, Mucor, Fusarium), yeasts (Cyberlindnera fabianii, Candida spp.), and bacteria (Bacillus spp., Staphylococcus aureus), which generally originate from post-baking contamination, production environment conditions, hygiene practices, storage conditions, as well as product formulation and packaging type. These microorganisms pose potential health risks through the production of carcinogenic and hepatotoxic mycotoxins, foodborne illness caused by bacterial enterotoxins, and the emerging threat of antifungal resistance in Aspergillus. Effective control measures include using essential oils as natural preservatives and controlling storage temperatures at both retail and production levels. This review emphasizes the importance of a multidisciplinary approach and combining multiple control strategies to minimize the risk of microbiological contamination in bread and ensure consumer food safety.