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Berahim, Zurairah
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Dentistry

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Cytotoxic Evaluation of Malaysian Kelulut Honey on Human Gingival Fibroblast Cell Line using MTT Assay Zi Yun, Chee; Mohd Nor, Nurul Hafizah; Berahim, Zurairah; Thirumulu Ponnuraj, Kannan
Journal of Dentistry Indonesia Vol. 28, No. 2
Publisher : UI Scholars Hub

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Kelulut honey or stingless bee honey is a type of honey produced by stingless bees of the Trigona species where the nest is found in living trees. Objective: The aim of this study was to evaluate the cytotoxic potential of Malaysian Kelulut honey by employing MTT assay on a human gingival fibroblast cell line. Methods:Human gingival fibroblast cell line was cultured in minimal essential medium alpha (α-MEM) with 10% foetal bovine serum and 1% penicillin-streptomycin solution in a 5% CO2 incubator at 37°C in a humidified atmosphere. The cells were seeded at a cell density of 5x103 cells/well in a 96-well culture plate for 24 hours. The cells were treated with seven different concentrations (200, 100, 50, 25, 12.5, 6.25, and 3.125mg/ml) of Malaysian Kelulut honey and incubated in a CO2 incubator. The negative control comprised cells treated with growth media alone. The cell viability was assessed using MTT assay at 24, 48, and 72 hours. The test plate was shaken using a microplate shaker and the absorbance of the solution was measured at 570nm using an ELISA reader with the Magellan software. Statistical analysis of the data was carried out using Kruskal-Wallis test and SPSS 24.0.0 for Windows. A p value Results: There was no cytotoxic effect of Malaysian Kelulut honey on HGF-1 based on the MTT assay at different concentrations and at different time points tested as the cell viability was above 70%. The highest percentage of cell viability at all three different durations of treatment were observed at 3.125mg/ml, whereas the lowest cell viability was observed at 200mg/ml of Kelulut honey concentration. However, statistically significant differences were seen between some of the concentrations at various time points. Conclusion: Since the cell viability of HGF-1 treated with Malaysian Kelulut honey was more than 70% at all concentrations ranging from 3.125mg/ml to 200mg/ml at three different time points (24, 48 and 72 hours), Malaysian Kelulut honey can be considered as non-cytotoxic on human gingival fibroblasts based on MTT assay under the present test conditions.
Perceived Stress, Severity of Xerostomia, and Periodontal Status in Undergraduate Dental Students Muzhaffar, Engku Ahmad; Berahim, Zurairah; Abdul Wahab, Norsila; Saddki, Norkhafizah
Journal of Dentistry Indonesia Vol. 30, No. 2
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Dentistry is a highly stressful program; stress is associated with xerostomia and periodontal disease. Objective: This study aims to investigate the association of perceived stress, severity of xerostomia, and periodontal status in dental students. Methods: This was a two-phase cross-sectional study of 245 Universiti Sains Malaysia (USM) undergraduate dental students. Phase 1 involved administration of the Perceived Stress Scale (PSS-10) and Summated Xerostomia Inventory (SXI). In phase 2, the Community Periodontal Index (CPI) was performed on 150 students to determine their periodontal status. Results: Mean PSS-10 and SXI scores were 19.6 (SD 5.47) and 7.9 (SD 2.04), respectively. Only 7.3% students had healthy periodontium while 17.3% had gingival bleeding, 65.3% had calculus, 6.7% had shallow pockets, and 3.3% had deep pockets. The association of perceived stress, severity of xerostomia, and periodontal status was not significant. Nevertheless, a significant positive correlation was found between PSS-10 and SXI scores (r = 0.318, p < 0.01). Conclusion: Most USM dental students were affected by some degree of periodontal disease, but it was not associated with perceived stress or severity of xerostomia. Students with higher perceived stress had more severe xerostomia. Information from this study could be utilised by the dental school in planning towards providing a stress-free training environment.
Knowledge, Attitude, Oral Hygiene Practice and Self-Perception on Periodontal Health among Older Adult Population in Malaysia Edwin Thomas, Annabel Shanta; Taib, Haslina; Berahim, Zurairah; Zainuddin, Siti Lailatul Akmar; Mohd Adnan, Munirah
Journal of Dentistry Indonesia Vol. 31, No. 3
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Objective: This study aimed to assess the knowledge, attitude, oral hygiene practice, and self-perception of periodontal health among the older adult population in Malaysia and its association with demographic factors. Methods: An online questionnaire assessing their knowledge, attitude, oral hygiene practices, and self-perception of periodontal disease was distributed to adults aged 50 years and above. Results: 397 respondents participated. The mean knowledge score was 1.36 (SD 0.92), significantly associated with gender, ethnicity, education, and occupation. About 63% of respondents know that poor oral hygiene is related to general health and is significantly associated with ethnicity (p = 0.016) and occupation (p = 0.009). Most respondents showed good oral hygiene practice but visited the dentist only when necessary (45%). Many of them have positive perceptions of their gum health status (81.6%) and are significantly associated with ethnicity (p = 0.007), education level, and occupations. Conclusion: This study showed inadequate knowledge about periodontal health among older adults. The attitude, practices, and self-perception of periodontal disease are mainly associated with ethnicity, educational levels, and occupations. Awareness of periodontal disease should be reinforced among older adults for better quality of life.
Prevalence of Peri-implantitis and Assessment of Oral Health-related Quality of Life Among Patients with Dental Implants at Hospital Universiti Sains Malaysia Ang, Esther Li-Ching; Berahim, Zurairah; Abd Rahman, Normastura; Hassan, Akram; Zainuddin, Siti Lailatul Akmar
Journal of Dentistry Indonesia Vol. 32, No. 1
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Patient-reported outcomes about peri-implantitis are lacking among the Asian population, impacting decision-making in dental implant therapy. Objectives: This study aimed to assess the prevalence of peri-implantitis among Malaysian adults, and its impact on oral health-related quality of life (OHRQoL). Methods: Randomly selected 105 participants (adults aged 18 and above) having 265 implants were examined and diagnosed as with or without peri-implantitis (PI). Clinical examinations, including peri-implant probing depth, bleeding, and suppuration on probing with radiographic bone loss, were used to determine peri-implant status. OHRQoL of all participants were recorded using the short version of the Malaysian Oral Health Impact Profile [S-OHIP(M)] and reported as prevalence and severity of impact scores. Results: Prevalence of PI was 20.95% (95%CI 13.17-28.74) at the patient level and 11.7% (95%CI 7.83-15.57) at the implant level. Participants with PI had significantly greater percentages (p < 0.05) reporting impacts ‘fairly often’ or ‘very often’ in the dimensions ‘functional limitation’ and ‘handicap’ with their respective sub-items ‘difficulty chewing’ and ‘spending money.’ Both groups showed no significant difference in impact severity across items and domains. Although total S-OHIP(M) scores were higher in the PI group, the difference was not significant (p = 0.105). Conclusion: Peri-implantitis is relatively prevalent at 20%, but its impact on OHRQoL is minimal.
Co-Authors Abd Rahman, Normastura Abdul Wahab, Norsila Akram Hassan, Akram Ang, Esther Li-Ching Edwin Thomas, Annabel Shanta Mohd Adnan, Munirah Mohd Nor, Nurul Hafizah Muzhaffar, Engku Ahmad Norkhafizah Saddki Siti Lailatul Akmar Zainuddin, Siti Lailatul Akmar Taib, Haslina Thirumulu Ponnuraj, Kannan Zi Yun, Chee