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All Journal AGROEKOTEKNOLOGI JURNAL ILMU PERTANIAN Jurnal Penelitian Karet Jurnal Agro Estate International Journal of Economic, Business, Accounting, Agriculture Management and Sharia Administration (IJEBAS) Warta Perkaretan
RADITE TISTAMA
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Environmental Science Materials Science & Nanotechnology

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MOLECULAR CHARACTERS OF ENDOPHYTIC FUNGI ISOLATE FROM RUBBER LEAVES (Hevea brasiliensis) Syamsafitri; Darma Bakti Nasution; Lisnawita; Radite Tistama
International Journal of Economic, Business, Accounting, Agriculture Management and Sharia Administration (IJEBAS) Vol. 2 No. 4 (2022): August
Publisher : CV. Radja Publika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54443/ijebas.v2i4.1245

Abstract

Endophytic microbes can be isolated from various types of plants, one of which is from rubber leaves (Hevea brasiliensis). Rubber plants have been planted widely, which means they have the ability to adapt to environmental conditions. Apart from that, currently many rubber clones have been produced which of course harbor potential microbes in them. The potential presence of endophytic fungi can be utilized to support the growth of rubber plants. Isolation of endophytic fungi from rubber plants which have potential as biological agents for controlling diseases, one of which is leaf fall disease. The aim of this research was to determine the diversity of endophytic fungal isolates associated with rubber clone leaves using the DNA extraction method from endophytic fungi from rubber leaves. The results of PCR amplification of the genome DNA of 5 endophytic fungal isolates using primers ITS1 and ITS4 produced one DNA fragment with a size of 612 bp for Aspergillus sp. (BB1) 598 bp for Aspergillus flavus (BB2), 637 bp for Trichoderma asperelum (BB4), 610 bp for Aspergillus sp (KI1) and 621 bp for Aspergillus flavus in isolate KI3 Based on the results of BLAST analysis of the DNA fragment sequence with the fungal isolate sequence Other endophytes in the National Center for Biotechnology Information (NCBI) GeneBank show that the sequence similarity between the isolates analyzed is 99-100%.
Co-Authors Ade Fipriani Lubis Ahmad Dahlan Arief Rachmawan Atminingsih Atminingsih Atminingsih Atminingsih Cici Indriani Dalimunthe Cici Indriani Dalimunthe DARMA BAKTI NASUTION Dita Amelia Novariza Elia Wike Wijayanti Wijaya Irwan Saban Iwandani SINURAT JUNAIDI JUN Junaidi Junaidi Lahmuddin Lubis Lisnawita Mochlisin Andriyanto Muhamad Rizqi Darojat Muhammad Rizqi Darojat Suzanna Fitriany Sitepu, Suzanna Fitriany Syamsafitri Tri Rapani Febbiyanti Yudiarto Sarsono Zaida Fairuzah