Article Per Year (5 Year)
p-Index From 2021 - 2026
1.066
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Alam dan Lingkungan Biosaintifika: Journal of Biology & Biology Education Jurnal Riset Akuakultur Jurnal Pembelajaran dan Biologi Nukleus BioEksakta : Jurnal Ilmiah Biologi Unsoed
Winanda, Witri
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Veterinary

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp. Winanda, Witri; Batubara, Irmanida; Lestari, Yulin
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.29981

Abstract

Antiobesity medication is available as therapeutic compounds that can reduce fat digestion by the inhibition of pancreatic lipase. Actinobacteria have the potency as source of bioactive compounds with various biological function including as pancreatic lipase inhibitor.  However, the potency of endophytic actinobacteria from Rhododendron spp. as source of pancreatic lipase inhibitor producer has not been reported yet. The aim of this study was to examine the potential of pancreatic lipase inhibitory activity of 23 endophytic actinobacteria from Rhododendron spp.; to characterize their colony based on morphology and molecular analysis. Screening test of pancreatic lipase inhibitor was conducted using the supernatant of endophytic actinobacteria, lipase pancreatic porcine (L3126) and p-nitrophenyl butyrate. The supernatant of selected isolates was extracted using ethyl acetate. The result showed that various inhibitory activities ranging between 0.00 until 91.69%. There were 11 out of 23 isolates that have potential as pancreatic inhibitor. Amongst them, the extract of four selected isolates, i.e. RZP 1.3, RSSB 3.2, RSS 2.1, and RJB F3.2 demonstrated inhibitory percentage of more than 80%. The RJB F3.2 extract showed to have IC50 value by 431.48 µg mL-1 compared to control, i.e. Xenical (89.07 µg mL-1). Phytochemical analysis exhibited that the extract of the selected isolates contained alkaloid which may function as pancreatic lipase inhibitor. Based on the morphological character, the selected isolates have various morphological colonies and 16S rRNA gene sequence revealed the sequence homology to Streptomyces spp. The data clearly indicate that endophytic actinobacteria from Rhododendron spp. have potency as pancreatic lipase inhibitor producer and further studies could be explored for the development of antiobesity agent.
The Potential of Various Matoa Plant (Pometia pinnata) Extracts as Antibacterial Agents: Systematic Literature Review Sarima, Sarima; Pistanty, Mingle A; Fathia, Mona; Winanda, Witri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7505

Abstract

Background: Bacteria in nature can have various effects on human life, including the development of diseases. Consequently, it is necessary to regulate or inhibit the growth of harmful bacteria. One method of doing so is through the use of natural ingredients, such as Matoa (Pometia pinnata). This study aims to provide a literature review on the potential of various Matoa plant extracts as antibacterials. Methodology: The investigation was conducted using the PRISMA approach, which involved the systematic literature review method. Electronic databases, including PubMed, ScienceDirect, Scopus, and Google Scholar, were searched from 2010 to 2024. "Pometia pinnata" is a search term that encompasses " Pometia pinnata as antibacterial" and "Antibacterial Activity of Pometia pinnata." A qualitative analysis was conducted on 22 articles that satisfied the inclusion criteria following the selection process. Findings: The review results indicated that the extracts of matoa's leaves, seeds, and bark contain active compounds, including flavonoids, tannins, saponins, alkaloids, and other phenolic compounds, which exhibit antibacterial activity. Despite the large potential, the majority of the research is still exploratory and has not yet progressed to the clinical trial stage. Consequently, additional, more intensive research is required to investigate the mechanism of action, toxicity, and potential clinical applications of herbal medicines derived from indigenous Indonesian plants. Contribution: This review highlights the antibacterial potential of matoa (Pometia pinnata) and underscores the need for further studies on its mechanism, toxicity, and clinical application, thereby guiding future research on Indonesian herbal medicine
Potensi Senyawa Bioaktif dari Bajakah Spatholobus littoralis Hassk. Sebagai Antikanker terhadap Sel MCF-7 secara In Vitro Habibi, Alif Rahman; Arfan, Alya Rahmaditya; Anwar, Khoirul; Azizah, Anggia; Fathia, Mona; Winanda, Witri; Berlyansyah, Azizul
Jurnal Ilmu Alam dan Lingkungan Vol. 16 No. 2 (2025): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bajakah (Spatholobus littoralis Hassk.) is a plant from the Angiospermae group, belonging to the Leguminosae family, that grows in Kalimantan. This plant contains secondary metabolites with potential anticancer activity. Cancer is characterized by uncontrolled cell proliferation beyond normal mechanisms, leading to abnormal tissue growth and invasion. It is one of the leading causes of death worldwide, including breast cancer. In 2022, approximately 2.3 million women were diagnosed with breast cancer, with 670,000 deaths recorded globally, while around 1.63 million women were still living with the disease by the end of the year. This study aimed to evaluate the anticancer activity of Bajakah Spatholobus littoralis Hassk. against MCF-7 breast cancer cells using the MTS assay. The results showed that Bajakah extract exhibited low toxicity toward the MCF-7 cell line, with an IC50 value of 1,098.77 ppm. Therefore, it can be concluded that Bajakah Spatholobus littoralis Hassk., tested with the MTS method, is not cytotoxic to breast cancer cells. Further studies are recommended to investigate its potential effects on other cancer cell types
Isolasi, Karakterisasi dan Aktivitas Antimikroba Bakteri Asam Laktat dari Ampas Susu Kedelai (Okara) Winanda, Witri; Maulidayanti, Sharfina; Nadila, Syifa Intan
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.18322

Abstract

Susu kedelai merupakan produk minuman hasil ekstraksi biji kedelai yang kaya protein nabati, asam amino serta komponen bioaktif lainnya. Proses dari pembuatan susu kedelai menghasilkan ampas kedelai (okara) yang umumnya belum dimanfaatkan secara optimal. Ampas kedelai mengandung nutrisi yang berpotensi untuk mendukung pertumbuhan bakteri asam laktat. Peran utama bakteri asam laktat adalah memproduksi senyawa antimikroba yang berperan dalam menghambat pertumbuhan bakteri patogen. Penelitian ini bertujuan untuk menguji potensi bakteri asam laktat yang dihasilkan dari isolasi ampas susu kedelai terhadap bakteri Enterococcus faecalis dan Acinetobacter baumannii. Penelitian ini menggunakan desain studi eksperimental. Populasi yang digunakan pada penelitian ini adalah limbah padat dari proses pembuatan susu kedelai. Teknik isolasi BAL dilakukan dengan metode spread plate menggunakan media MRSA yang diperkaya kalsium karbonat 1%. Karakterisasi BAL ditentukan melalui uji makroskopik, mikroskopik dan biokimia, sedangkan untuk uji aktivitas antimikroba menggunakan metode difusi sumuran menggunakan media Mueller Hinton Agar (MHA). Penelitian ini berhasil mengisolasi satu isolat yang sesuai dengan karakteristik bakteri asam laktat, yakni cocobacil, Gram positif, katalase negatif. Isolat tersebut yaitu AK yang tahan pada kondisi asam (pH 2,5) serta dapat tumbuh pada medium dengan kadar garam empedu 3%. Hasil uji aktivitas antimkroba dari ekstrak kasar isolat BAL tidak efektif dalam menghambat pertumbuhan bakateri patogen A. baumannii dan E. faecalis. Oleh karena itu perlu dilakukan penelitian lanjutan untuk mengoptimalkan kondisi fermentasi, melakukan ekstraksi dengan pelarut yang sesuai serta dilakukan identifikasi gen molekuler. Kata Kunci: Ampas susu kedelai, Antimikroba, E. faecalis, A. baumannii
EFFECT OF FERMENTED FIG FLOUR (Ficus racemosa L.) SUBSTITUTION ON PROTEIN AND LIPID RETENTION IN GIANT GOURAMI (Osphronemus goramy Lacepede, 1801) Fitra, Ramadani; Zakaria, Indra Junaidi; Febria, Fuji Astuti; Winanda, Witri; Fathia, Mona
Jurnal Riset Akuakultur Vol 20, No 4 (2025): Desember (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.4.2025.329-338

Abstract

Ficus racemosa has considerable potential as a plant-based ingredient for partially replacing soybean meal in diets for giant gourami. Fermenting fig flour with Effective Microorganisms-4 (EM-4) is expected to enhance protein quality and improve nutrient utilization. This study evaluated the effects of substituting artificial feed with fermented fig flour on protein and lipid retention in giant gourami cultured under aquaculture conditions. The experiment employed a completely randomized design with five dietary treatments containing 0%, 10%, 20%, 30%, and 40% fermented fig flour, each with five replicates. All diets were formulated to be isonitrogenous, with a crude protein content of 32%. Fig flour was aerobically fermented for 72 hours using EM-4 prior to diet formulation. A total of 250 juvenile giant gourami (initial body weight 15–20 g) were reared for 70 days and fed twice daily at a feeding rate of 3% of total biomass. Fish length and weight were recorded at 14-day intervals. Protein and lipid retention were determined based on proximate analyses conducted at the beginning and end of the experiment. The results showed that dietary inclusion of fermented fig flour significantly affected protein and lipid retention (p < 0.05). The highest protein retention (18.41 ± 0.86%) and lipid retention (36.03 ± 1.60%) were observed in fish fed the diet containing 30% fermented fig flour. These findings indicate that partial substitution of artificial feed with 30% fermented fig flour improves nutrient retention efficiency and represents a promising strategy for developing cost-effective and sustainable diets for giant gourami culture.Ficus racemosa memiliki potensi yang besar sebagai bahan pakan nabati untuk menggantikan sebagian tepung kedelai dalam pakan ikan gurami. Fermentasi tepung buah ara menggunakan Effective Microorganisms-4 (EM-4) diharapkan dapat meningkatkan kualitas protein dan memperbaiki pemanfaatan nutrien. Penelitian ini bertujuan untuk mengevaluasi pengaruh substitusi pakan buatan dengan tepung buah ara terfermentasi terhadap retensi protein dan lipid pada ikan gurami yang dibudidayakan dalam lingkungan terkontrol. Percobaan menggunakan rancangan acak lengkap dengan lima perlakuan pakan yang mengandung tepung buah ara terfermentasi sebesar 0%, 10%, 20%, 30%, dan 40%, masing-masing dengan lima ulangan. Seluruh pakan diformulasikan secara isonitrogenus dengan kadar protein kasar sebesar 32%. Tepung buah ara difermentasi secara aerob selama 72 jam menggunakan EM-4 sebelum diformulasikan ke dalam pakan. Sebanyak 250 ekor benih ikan gurami (bobot awal 15–20 g) dipelihara selama 70 hari dan diberi pakan dua kali sehari dengan dosis 3% dari total biomassa. Panjang dan bobot ikan diukur setiap 14 hari. Retensi protein dan lipid ditentukan berdasarkan analisis proksimat yang dilakukan pada awal dan akhir percobaan. Hasil penelitian menunjukkan bahwa penambahan tepung buah ara terfermentasi dalam pakan berpengaruh nyata terhadap retensi protein dan lipid ikan gurami (p < 0,05). Retensi protein tertinggi (18,41 ± 0,86%) dan retensi lipid tertinggi (36,03 ± 1,60%) diperoleh pada ikan yang diberi pakan dengan kandungan 30% tepung buah ara terfermentasi. Temuan ini menunjukkan bahwa substitusi sebagian pakan buatan dengan 30% tepung buah ara terfermentasi dapat meningkatkan efisiensi retensi nutrien dan merupakan strategi yang menjanjikan untuk pengembangan pakan ikan gurami yang lebih ekonomis dan berkelanjutan.
Inventarisasi Spesies Jamur Basidiomycota di Jalur Track Telaga Biru, Batam, Kepulauan Riau Anwar, Khoirul; Fathia, Mona; Winanda, Witri; Fitra, Ramadani; Fazri, Muhammad Adrul
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 8 No 1 (2026): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2026.8.1.19770

Abstract

Jamur pengurai kayu dari kelompok Basidiomycota berperan penting dalam siklus unsur hara hutan tropis Batam, namun data dasar makrofungi di Pulau Batam masih terbatas. Penelitian ini bertujuan untuk menginventarisasi jamur liar yang ada di Pulau Batam. Metode pengumpulan data dilakukan dengan cara survey metode jelajah. Jamur yang ditemukan difoto dan diambil sampelnya dibawa ke Laboratorium untuk dilakukan pengamatan lebih lanjut. Survei jelajah selama satu hari dilakukan pada 13 Juli 2025 di Jalur trek Telaga Biru. Hasil Penelitian mencatat tujuh taksa pada substrat kayu lapuk, yaitu Dacrymyces (= Dacryopinax) spathularia, Pycnoporus cf. (sanguineus/puniceus), Pleurotus sp., Trametes versicolor, Gymnopilus cf. subpurpuratus, Ganoderma applanatum, serta polypore lainnya. Komposisi ini mencerminkan habitat yang dikuasai perombak lignoselulosa. Penelitian ini berhasil mengungkat data awal jamur Basidiomycetes yang ditemukan dan memerlukan penelitian lebih lanjut mengenai fungsi ekologis dan potensi pemanfaatan jamur pengurai kayu setempat. Kata kunci: Basidiomycota; Batam;; makrofungi; inventarisasi; Trek Telaga Biru
Co-Authors Alya Rahmaditya Arfan Anggia Azizah Berlyansyah, Azizul Fathia, Mona Fazri, Muhammad Adrul Fitra, Ramadani Fuji Astuti Febria Habibi, Alif Rahman Indra Junaidi Zakaria Irmanida Batubara Khoirul Anwar Mingle A Pistanty, Mingle A Nadila, Syifa Intan Sarima, Sarima Sharfina Maulidayanti YULIN LESTARI