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All Journal Nusantara Science and Technology Proceedings Society : Jurnal Pengabdian dan Pemberdayaan Masyarakat
Pendrianto
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Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Cell Line Authentication (CLA) using Short Tandem Repeat (STR) Method on Breast Cancer cell line MCF-7 Pendrianto; Muhammad Samsul Mustofa; Silviatun Nihayah
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series B
Publisher : Future Science

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Abstract

Research on human diseases, especially in cancer begins with in vitro studies using cell lines. However, cell lines grown in vitro can occur cross-contamination or even miss-identification. This requires a cell line authentication (CLA) procedure using a molecular approach. In this process, analysis of allele variance from several loci is needed to ensure that this variant matches the expected allele. The STR method can be used to authenticate cell lines, because it can compare allele profiles with a sample of known standard cell lines, and also able to provide simple, inexpensive, and very specific genetic "fingerprints" on cell lines. It is necessary to authenticate cell lines in collections of cell lines in the Central Laboratory for Stem Cell Studies at the YARSI University Research Institute, so that in vitro and in vivo studies on anticancer, antioxidants, aging, and longevity involving cell lines will produce valid outcomes in future research. The research method was laboratory experimental. breast cancer cell lines MCF-7, grown on growth media containing serum and antimicrobial-antimycotic. Genomic DNA was isolated by Maxwell® RSC Whole Blood DNA Kit. PCR process using GlobalFiler™ PCR amplification Kit (Applied Biosystem) and fragment analysis was analyzed with Genetic Analyzer 3500. The preliminary results of fragment analysis on two MCF-7 lines originating from two different places indicated that these cell lines were inauthentic. Possibly due to cross contamination during activity in the laboratory. This makes the two cell lines unusable as materials in in vitro studies. There are limitations to this study because it only analyzed 1 batch of subcultures for each cell line. Further research is needed in the entire batch of culture collection in the Central Laboratory for Stem Cell Studies at the YARSI University Research Institute.
Program Sosialisasi Halal dan Thayyib dari Pendekatan Psikologi dan Islam bagi Pelaku UMKM di Kecamatan Cempaka Putih Jakarta Pusat Karimulloh; Caninsti, Riselligia; Nursanti, Ade; Suseno, Dedy; Pendrianto
Society : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol. 6 No. 1 (2025): Vol. 6 No. 1, Oktober 2025
Publisher : Universitas Dinamika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37802/society.v6i1.836

Abstract

For Muslims, halal and thayyiban in food and drink is an obligation. The importance of understanding for UMKM (Micro, Small and Medium Enterprises) regarding the use of halal raw materials in making food products must be increased, so that these business actors only make halal and thayyib food. Based on Law of the Republic of Indonesia Number 33 of 2014 concerning Halal Product Guarantees, every food, drink and even medicine product circulating in Indonesia is required to have a Halal Certificate. The importance of understanding the selection of halal raw materials and thayyib is an important support in the halal product certification process. Halal and thayyib socialization program from a psychological and Islamic approach aims to increase the knowledge of micro, small and medium enterprises. Then, they can register their products to obtain a Halal Certificate. Halal certificate is one of criteria for the public to consume food and drinks, so that the general public does not worry about the food or drinks they consume. The target of this program is JP42 and Optima Food Court YARSI business owner in Central Jakarta. The program was implemented on Wednesday - Thursday, 24-25 January 2024. The result on pre-test and post-test  were processed using JASP Wilcoxon Signed Rank Test. Based on statistical analysis, the results showed a significant increase in participants’ understanding oh halal and thayyib raw materials, how to process them, and how to obtain them. With this increase in knowledge, it is hoped that business actors can register their business products to obtain a Halal Certificate.
Co-Authors Ade Nursanti Karimulloh, Karimulloh Muhammad Samsul Mustofa Riselligia Caninsti, Riselligia Silviatun Nihayah Suseno, Dedy