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Susanto, Vetra
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ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA FROM BREAST MILK Indrayati, Sri; Susanto, Vetra
JURNAL KATALISATOR Vol. 9 No. 2 (2024): Jurnal Katalisator Volume 9 No.2, Oktober 2024
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v9i2.3099

Abstract

Breast milk (breast milk) is one of the sources of Lactic Acid Bacteria (BAL), which functions to maintain the balance of the baby's digestive microflora. BAL derived from breast milk has high viability in the digestive tract and is more adaptive if it comes from the human body. Some BALs are also called probiotics. If Lactic Acid Bacteria Isolate is obtained from breast milk, it can be used as a probiotic candidate in the prevention of stunting and infectious diseases in children. This research applies new biotechnology to produce a food product that is healthy, contains probiotics, and is preferred by the community. The purpose of the study is to isolate and test the probiotic potential of BAL strains isolated from breast milk so that it can have the potential to be applied in the food industry. This research method is an exploratory study to obtain Lactic Acid Bacteria isolate from Breast Milk. The results of the study showed that 14 Lactic Acid Bacteria Isolate was obtained which was shown by the formation of a clear zone area around the colony. Based on the results of biochemical identification, of the 14 BAL isolates that have been successfully identified biochemically, consisting of several genera of BAL, namely Lactobacillus dan Pediococcus.
THE REMAINING CHEMICAL COMPOUNDS OF THE BODY'S METABOLISM ARE REDUCED THROUGH THE HEMODIALYSIS METHOD Susanto, Vetra; Dewi AS, Mutia; Suryani, Suryani; Suriani, Endang; Dasa Wardhani, Sri Muri
JURNAL KATALISATOR Vol. 10 No. 1 (2025): Jurnal Katalisator ( On Progres)
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v10i1.3450

Abstract

The accumulation of metabolic residual chemical compounds, such as urea, and creatinine, is a major consequence of decreased glomerular filtration function in patients with chronic kidney disease (CKD). Increased concentrations of these compounds trigger internal homeostasis disorders and accelerate the progression of metabolic complications. Hemodialysis is a kidney replacement therapy that aims to eliminate toxic compounds and maintain the body's biochemical balance. The purpose of this study is to evaluate the effectiveness of hemodialysis in reducing urea, creatinine, and uric acid levels in CKD patients. The pre-post test research involved 30 stage V CKD patients who underwent routine hemodialysis at the Regional General Hospital (RSUD) Padang Panjang, West Sumatra. Venous blood samples were taken 15 minutes before and after a single hemodialysis session. Urea levels were analyzed using the urease-GLDH method, creatinine using the kinetic Jaffé method, and uric acid using the uricase-POD method. The data were analyzed using a paired t-test with a significance level of p<0.05.The results were obtained The average urea level decreased from 182.6 ± 28.7 mg/dL it was concluded that Hemodialysis significantly reduced the level of chemical compounds left over from the body's metabolism in patients to 76.3 ± 16.9 mg/dL (p<0.001), creatinine from 11.8 ± 2.9 mg/dL to 4.9 ± 1.3 mg/dL (p<0.001), and uric acid from 8.5 ± 1.8 mg/dL to 5.2 ± 1.1 mg/dL (p<0.001). It can be concluded that hemodialysis significantly reduces the level of chemical compounds left over from the body's metabolism in advanced CKD patients. Hemodialysis maintains biochemical homeostasis and reduces the risk of systemic complications.
HUBUNGAN TINGKAT PENGETAHUAN DAN STIMULASI IBU DENGAN PERKEMBANGAN BALITA DI PUSKESMAS BATU AJI Andolina, Nuari; Suciana, Sri; Susanto, Vetra; Nurhanisya, Sri
HUMAN CARE JOURNAL Vol 9, No 1 (2024): Human Care Journal
Publisher : Universitas Fort De Kock

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32883/hcj.v9i1.2899

Abstract

Perkembangan dan peningkatan kualitas hidup anak merupakan upaya penting untuk masa depan Indonesia yang lebih baik. Stimulasi merupakan salah satu aspek kebutuhan dasar anak (ASAH). Tujuan umum dari penelitian ini untuk mengetahui hubungan tingkat pengetahuan dan stimulasi ibu dengan perkembangan balita 12-59 bulan di Puskesmas. Penelitian ini merupakan kuantitatif dengan desain cross sectional. Pengumpulan data menggunakan kuesioner. Pelaksanaan penelitian dilakukan pada bulan Mei. Populasi dan sampel penelitian ini balita usia 12-59 bulan. pengambilan sampel dengan menggunakan rumus Lameshow sebanyak 39 balita, Data di analisa secara univariat dan bivariat. Berdasarkan hasil penelitian didapatkan lebih dari separoh responden dengan pengetahuan tinggi yaitu 55,6 %. Variabel tingkat pengetahuan berhubungan dengan perkembangan balita dan nilai OR = 2,119 yang artinya tingkat pengetahuan yang tinggi mempunyai peluang dua kali untuk perkembangan balita, dan variabel stimulasi ibu berhubungan dengan perkembangan balita dengan dan nilai OR = 5,200 yang artinya stimulasi ibu mempunyai peluang lima kali untuk perkembangan balita. Berdasarkan hasil penelitian maka dapat disimpulkan bahwa variabel tingkat pengetahuan dan stimulasi ibu berhubungan dengan perkembangan balita 12-59 bulan di Puskesmas. Diharapkan kepada Puskesmas melakukan pemantauan SDDIDTK kepada balita agar dapat mengetahui jika terjadi penyimpangan perkembangan.Kata Kunci : Perkembangan Balita, Stimulasi Ibu, Tingkat Pengetahuan
Isolasi dan Identifikasi Molekuler Candida albicans dari Sekret Keputihan Menggunakan Metode PCR Sophia, Anggun; Suraini, Suraini; Susanto, Vetra; Niken, Niken
Jurnal Kesehatan Medika Saintika Vol 16, No 2 (2025): Desember 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i2.30704

Abstract

Candida albicans is one of the major pathogenic fungi responsible for pathological vaginal discharge in women. This study aimed to isolate and identify C. albicans from vaginal discharge samples using conventional methods and molecular confirmation based on Polymerase Chain Reaction (PCR). A total of five vaginal discharge samples were collected and cultured on selective media. The isolation results showed that four samples (SC2–SC5) exhibited colony characteristics consistent with C. albicans, while one sample (SC1) demonstrated morphological features suggestive of the genus Aspergillus. Conventional identification through macroscopic and microscopic examination, as well as the germ tube test, confirmed that the four isolates were strong candidates for C. albicans. Molecular identification using specific primers targeting the Internal Transcribed Spacer (ITS) region indicated that only one isolate produced a distinct DNA band at approximately ±600 bp, which corresponds to the specific ITS fragment size for C. albicans. The SC3 isolate was confirmed as C. albicans based on molecular analysis. These findings highlight the importance of PCR-based confirmation to improve the accuracy of fungal identification, particularly in cases of pathological vaginal discharge.
Co-Authors Andolina, Nuari Anggun Sophia Dasa Wardhani, Sri Muri Dewi AS, Mutia Indrayati, Sri Niken Niken Nurhanisya, Sri Suciana, Sri Suraini, Suraini Suriani, Endang Suryani Suryani