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All Journal Menara Perkebunan JNANALOKA Biocaster : Jurnal Kajian Biologi
Pangestika, Intan Widya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Social Sciences Transportation Other

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Prediksi In Silico Senyawa Berpotensi Anti-Kolesterol pada Kopi Robusta (Coffea canephora Pierre ex A. Froehner) Aida Fasha, Gusti Nur; Pangestika, Intan Widya
JNANALOKA Vol. 06 No. 01 Maret Tahun 2025
Publisher : Lentera Dua Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36802/jnanaloka.2025.v6-no1-1-11

Abstract

Kopi robusta (Coffea canephora Pierre ex A. Froehner) merupakan salah satu komoditas penting di Indonesia yang memiliki potensi besar dalam bidang kesehatan, termasuk sebagai agen anti-kolesterol. Penelitian ini bertujuan untuk memprediksi senyawa dalam kopi robusta yang berpotensi sebagai anti-kolesterol menggunakan pendekatan in silico. Senyawa yang terkandung dalam kopi robusta diidentifikasi melalui basis data KNApSAcK, kemudian dianalisis menggunakan PASS Online untuk memprediksi aktivitasnya sebagai agen anti-kolesterol. Selanjutnya, prediksi target biologis dilakukan menggunakan SwissTargetPrediction, dan kecocokan farmakokinetik senyawa dianalisis berdasarkan Lipinski’s Rule melalui SwissADME. Hasil analisis menunjukkan bahwa beberapa senyawa memiliki potensi aktivitas tinggi sebagai agen anti-kolesterol, di antaranya delta7-Avenasterol, delta7-Stigmastenol, (3beta,5alpha)-Stigmastan-3-ol, (-)-beta-Sitosterol, dan Clerosterol, yang diprediksi berinteraksi dengan enzim sitokrom P450 dan reseptor nuklear yang berperan dalam regulasi kolesterol. Namun, senyawa ini memiliki nilai lipofilisitas tinggi, yang dapat mempengaruhi penyerapannya dalam tubuh. Sebaliknya, senyawa 2,3-Butanedione, Toluene, 2,3-Dimethylpyrazine, Phenol, dan Pyridine memenuhi semua kriteria Lipinski’s Rule, menunjukkan potensi lebih besar sebagai kandidat obat yang dapat dikembangkan lebih lanjut. Penelitian lanjutan diperlukan dalam bentuk uji in vitro dan in vivo guna mengonfirmasi efektivitas serta mekanisme kerja senyawa-senyawa ini dalam menurunkan kadar kolesterol. Selain itu, optimasi formulasi farmasi dan studi toksikologi perlu dilakukan untuk menjamin keamanan dan efektivitasnya sebagai agen terapi anti-kolesterol. Penelitian ini diharapkan dapat menjadi dasar dalam pengembangan obat berbasis senyawa alami dari kopi robusta, mendukung pemanfaatan komoditas lokal Indonesia dalam bidang kesehatan.
Relationship between altitudes, morphological traits, and biochemical compositions of Coffea canephora Pierre ex A. Froehner in Temanggung, Indonesia Pangestika, Intan Widya; Susilowati, Ari; Purwanto, Edi; Gunawan
Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.621

Abstract

Among various edaphoclimatic factors influencing coffee bean quality, altitude is one of the primary factors that should be taken into account. This study aims to explore the relationship between altitude, morphological traits, and biochemical composition of robusta coffee in Temanggung Regency, the largest coffee-producing area in Central Java Province, Indonesia. Research sites were purposively selected and categorized into two altitude groups: GS, PS, and GN, located at circa 600 m a.s.l., and GT, WO, and TG, located at circa 900 m a.s.l. A total of 15 morphological traits were observed, consisting of eight vegetative and seven generative traits. Caffeine content was measured following the AOAC procedure using UV-Vis spectrophotometry, while brew acidity was evaluated using a pH meter. Several morphological traits of Temanggung robusta coffee demonstrated significant relationships with altitude. Canopy and stem diameter showed negative correlations with altitude, with correlation coefficients of -0.366 and  -0.408, respectively. Conversely, fruit width               (r = 0.041), bean length (r = 0.049), and bean thickness (r = 0.047) exhibited positive correlations. Regarding biochemical composition, caffeine content stood out by displaying a strong positive correlation with altitude (r = 0.816). Additionally, several morphological traits, including the number of primary branches, the number of productive branches, the number of fruits per bunch, and traits related to fruits and beans, appear to be advantageous for selection and breeding programs. Understanding these relationships provides valuable insights for developing superior Temanggung robusta coffee plants adapted to specific altitudinal conditions.
Insights into Evolutionary Relationships of Proboscis Monkeys Using In Silico Mitochondrial DNA Analysis Sasmita, Rani; Badruzsaufari, Badruzsaufari; Pangestika, Intan Widya
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.688

Abstract

This study investigates the evolutionary position of the proboscis monkey (Nasalis larvatus) within the Colobinae subfamily using in silico phylogenetic analysis of three mitochondrial protein-coding genes (COI, COII, and COIII). Seventy sequences retrieved from GenBank were aligned and analyzed using the Maximum Likelihood method with 1,000 bootstrap replicates. All genes exhibited conserved lengths and strong AT bias (59,21-60,68%), reflecting typical mitochondrial genome characteristics shaped by asymmetric replication and purifying selection. Genetic distance analyses revealed high intraspecific consistency in Nasalis larvatus, while demonstrating marker dependent variation in phylogenetic resolution. COII and COIII provided clearer intergeneric separation compared to the more conserved COI marker. Phylogenetic reconstruction showed topological variation among markers, however, a consistent evolutionary signal emerged, with Nasalis larvatus clustering robustly with Simias concolor across all gene trees. This pattern supports the monophyly of the odd nosed monkey clade and aligns with genome scale phylogenomic evidence. Overall, this study highlights the limitations of single gene analyses and emphasizes the importance of multi marker or genome scale approaches for resolving complex evolutionary relationships. These findings provide foundational insights into the evolutionary history of Nasalis larvatus and reinforce the relevance of mitochondrial markers in primate conservation genetics.
Co-Authors ARI SUSILOWATI Badruzsaufari Badruzsaufari Edi Purwanto Fasha, Gusti Nur Aida Gunawan Sasmita, Rani