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All Journal Jurnal Fitopatologi Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Bioeksperimen: Jurnal Penelitian Biologi Jurnal Teknologi Laboratorium Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Journal of English Education, Linguistic and Literature Menara Perkebunan Dedikasi Saintek Jurnal Pengabdian Masyarakat Molekul: Jurnal Ilmiah Kimia Berkala Ilmiah Biologi Biosaintifika: Journal of Biology & Biology Education Journal of Health Research and Technology DEDIKASI SAINTEK Jurnal Pengabdian Masyarakat
ARI SUSILOWATI
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds Rahmawati, Sri Indah; Yunus, Ahmad; Susilowati, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11234

Abstract

Artemisia annua is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. Gloriosa superba is a plant that contains high colchicine compounds, especially on the seeds. Gloriosa superba extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of Gloriosa superba seeds was used as a mutagen for Artemisia annua. The aim of this study was to determine the size and density of Artemisia annua stomata soaked in water extract of Gloriosa superba seeds as a mutagen. Extraction of Gloriosa superba seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). Artemisia annua sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of Gloriosa superba seeds and soaking time of Artemisia annua sprouts. Measurements of stomatal length, width and density were conducted in epidermis of Artemisia annua leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of Gloriosa superba seeds proved the effects on stomatal size and density of treated plants.
Aktivitas Antibakteri Kulit Buah Karika Dieng terhadap Shigella flexneri dan Escherichia coli Novalina, Dhiah; Sugiyarto, Sugiyarto; Susilowati, Ari
Jurnal Teknologi Laboratorium Vol 7 No 2 (2018): 2018 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (680.767 KB) | DOI: 10.29238/teknolabjournal.v7i2.137

Abstract

Karika is an endemic plant of Dieng Plateau. The leaf of Karika has been studied to have antibacterial activity against bacteria that cause diarrhea, while the peels are removed or used as a mixture of animal feed. This study aims to determine the antibacterial activity of Karika peels against bacteria that cause poisoning, i.e Shigella flexneri, and Escherichia coli. The sample fractionated to obtain the fraction of n-hexane and ethyl acetate. The fractions were tested for their antibacterial activity against Shigella flexneri and Escherichia coli by the cup-plate method. The results showed that the ethyl acetate fraction (ethyl acetate 50%) had the highest inhibition on Shigella flexneri and Escherichia coli compared with another concentration. Based on the study can be concluded that the peels of Karikahas antibacterial activity against Shigella flexneri and Escherichia coli.
Isolation and Identification of α-glycosidase Inhibitor Producer Endophytic Bacteria from Salak Pondoh Fruit (Salacca edulis) Susilowati, Ari; Dewi, Citra Praba Yunita; Sari, Siti Lusi Arum
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.21031

Abstract

Alpha-glycosidase inhibitors can delay the hydrolysis of oligosaccharide and disaccharide into glucose, which can prevent or treat hyperglycemia in diabetes mellitus. The rind and flesh of Salak Pondoh fruit are known to produce ?-glycosidase inhibitor compound. Endophytic bacteria that live in plant tissues potentially produce compounds such as in host plants. Exploration of endophytic bacteria from the rind and flesh of Salak Pondoh is one of the efforts to obtain isolates of bacteria producing ?-glycosidase inhibitors. The objective of this study is to isolate and identify endophytic bacteria producing inhibitor ?-glycosidase from rind and flesh of Salak Pondoh, and to know the activity of its ?-glycosidase inhibitor. Isolation of endophytic bacteria was done by inoculating surface-sterilized plant samples on Nutrient Agar (NA) medium. The inhibitory activity towards ?- glycosidase analysis was performed using the spectrophotometric method (? = 415 nm), with p-nitro phenyl ?-D-glucopyranoside as the substrate. Identification of the bacteria was performed based on the 16S rRNA gene sequence.  The sequencing was done at 1st Base Singapore and the obtained sequences were identified using the BLAST Nucleotide device on the NCBI website. In total, 6 bacterial isolates were obtained. The inhibitory activity ranged from 6.14-62.95% and the highest was generated by Kt-E isolates. The bacteria obtained were isolated Dt- A and Dt-B that represent genus Xanthomonas, Kt-E from genus Paenibacillus, Kt-I from genus Bacillus, Dm-A1 and Dm-A2 from family Enterobacteriaceae. The results confirm the potential of the endophyte bacteria of Salak Pondoh to be an alternative source of hyperglycemia medication.
BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM Klebsiella sp. ASR1 ENCAPSULATED WITH ALGINATE Hidayatullah, Muhammad Eka; Sajidan, .; Susilowati, Ari; Mkumbe, Baraka Stewart; Greiner, Ralf
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (910.584 KB) | DOI: 10.29122/jbbi.v7i1.2997

Abstract

Karakterisasi Biokimia Enzim Fitase Rekombinan (phyK) dari Klebsiella sp. ASR1 Yang Dienkapsulasi Dengan AlginatEnzim fitase melepas molekul fosfor pada atom C dari benzena Inositol fitat. Tetapi fitase memiliki kelemahan tidak mampu bertahan terhadap kondisi ekstrim dalam lambung nonruminansia. Solusi dalam penelitian ini yaitu fitase dienkapsulasi menggunakan alginat. Penelitian ini bertujuan mengkarakterisasi fitase setelah dienkapsulasi menggunakan alginate. Hasil penelitian ini yaitu fitase yang dienkapsulasi memiliki aktivitas tertinggi pada pH 6,0, sedangkan fitase tanpa enkapsulasi pada pH 5,0. Suhu optimum untuk aktivitas tertinggi fitase yang dienkapsulasi yaitu 70ºC, sedangkan fitase tanpa enkapsulasi 37ºC. Untuk perlakuan penambahan ion logam, aktivitas tertinggi fitase yang dienkapsulasi terjadi dengan penambahan 0,1 mM Fe2+ dan 1,0 mM Ca2+, sedangkan fitase tanpa enkapsulasi dengan penambahan 0,1 mM Fe2+. Berdasarkan hasil penelitian ini, fitase yang dienkapsulasi memiliki keunggulan lebih banyak dibandingkan dengan fitase tanpa enkapsulasi, karena mampu bertahan pada pH dan suhu tinggi, dan beberapa efek ion logam.Kata Kunci: alginat, asam fitat, enkapsulasi, fitase, fitase rekombinanABSTRACTPhytase enzymes release phosphorus molecules on the C atom from benzene inositol phytate. But phytase has the disadvantage of being unable to withstand extreme conditions in the non-ruminant stomach. The solution in this research was phytase encapsulated using alginate. This study aims to characterize phytase after being encapsulated using alginate. The results of this study were the encapsulated phytase had the highest activity at pH 6.0, while the unencapsulated phytase at pH 5.0. The optimum temperature for the highest activity of the encapsulated phytase was 70ºC, while the unencapsulated phytase 37ºC. For treatment of metal ion addition, the highest activity of the encapsulated phytase occurred with the addition of 0.1 mM Fe2+ and 1.0 mM Ca2+, while the unencapsulated phytase with the addition of 0.1 mM Fe2+. Based on the results of this study, the encapsulated phytase had more advantages compared to the unencapsulated phytase, as the former withstand high pH and temperature, and some metal ion effects.
AN ANALYSIS OF ENGLISH FOR PROFESSIONAL NURSES TEXTBOOK IN STIKES PEMKAB JOMBANG Noviana, I'in; Rosmaharani, Shanti; Susilowati, Ari
JEELL (Journal of English Education, Linguistics and Literature) English Department of STKIP PGRI Jombang Vol 7, No 2 (2021)
Publisher : STKIP PGRI Jombang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32682/jeell.v7i2.1836

Abstract

An English textbook has an important role in teaching and learning process, to support the learning process in the classroom. The purpose of this study is to identify and analyze the English for Specific Purposes of Nursing bachelor program and diploma program. This study was conducted by using qualitative approach and analyses in descriptive method. The samples were 92 respondents and was divided from 62 students of 3rd grade of nursing bachelor program and 30 students of 3rd from diploma of nursing of STIKES Pemkab Jombang who are getting the English subject in this semester and the sampling technique used total sampling. The instrument of this study used questionnaire and the interview guideline in form of closed – ended question. The data analysis was descriptive analysis in the form of number. The result of this study revealed that the English for Nursing Textbook was well designed and encouraged the students’ ability to improve the skills in Nursing English language. English subject help the students to communicate in English to support their career in field of nursing Education. Although this english textbook is good enough, there are some evaluations, such as provided the listening comprehension for listening activity, so the lecturers have to open a youtube to improve the students’ ability in listening and speaking.
Probe DNA sebagai Kandidat Biosensor Clavibacter michiganensis subsp. michiganensis pada Tanaman Tomat Hidayati, Muslihah Nur; Suranto; Susilowati, Ari
Jurnal Fitopatologi Indonesia Vol. 18 No. 3 (2022): Mei 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.3.125-133

Abstract

Probe DNA sebagai Kandidat Biosensor Clavibacter michiganensis subsp. michiganensis pada Tanaman Tomat Penelitian dilakukan untuk menguji teknik deteksi gen menggunakan DNA probe spesifik untuk mendeteksi gen tomA pada Clavibacter michiganensis subsp. michiganensis, bakteri penyebab kanker pada tomat. Probe dirancang menggunakan program Primer3Plus, dilabel dengan molekul nonradioaktif digoxigenin (DIG) dan digunakan pada metode hibridisasi dengan teknik dot blot. Sampel uji terdiri atas dua jenis, yaitu sampel DNA genom yang berasal dari kultur bakteri murni dan dari benih tomat yang diinfeksi buatan dengan C. michiganensis subsp. michiganensis. Sampel yang berasal dari kultur murni bakteri menunjukkan hasil hibridisasi positif pada semua tingkat konsentrasi DNA; sedangkan sampel yang berasal dari biji tomat hanya menunjukkan reaksi positif pada konsentrasi 10, 8, 6, dan 4 µg µL-1. Studi ini menyimpulkan bahwa probe yang dirancang berpotensi untuk digunakan dalam pengembangan metode deteksi C. michiganensis subsp. michiganensis pada biji tomat berbasis biosensor dan bersifat cukup spesifik karena tidak ada reaksi silang dengan kelompok bakteri bukan target.
Antimicrobial Activity of Natural Solid Soap with a Combination of Liquid Cheese Waste, Turmeric (Curcuma longa), and Dragon Fruit (Hylocereus polyrhizus) Peel Estikomah, Solikah Ana; Suranto, Suranto; Susilowati, Ari; Masykuri, Mohammad
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.8039

Abstract

Yoghurt whey is a product from fermented cheese liquid waste. Yoghurt cheese liquid waste has been known to contain lactoferrin. Lactoferrin functions as an antimicrobial. yoghurt made from whey has not been widely utilized as a solid soap ingredient. This research focuses on producing solid natural soap made of yoghurt whey with added natural dye turmeric and dragon fruit peel, soap quality testing, and analysis of the soap's antibacterial activity toward Staphylococcus aureus. The solid soap was processed through the saponification reaction between palm oil, coconut oil, zaitun oil, and canola oil with sodium hydroxide. Variations of yogurt whey used in this study yoghurt 25%,75%,100% whey) was added natural dye (turmeric and dragon fruit peel). The quality test was done by determining free alkali, water content, pH, and foam height. Antibacterial activity test using the well diffusion method. The result shows that the quality test yoghurt soap meets with INS (Indonesia National Standard) 2016, for all the criteria. Performs inhibition against bacteria Staphylococcus aureus. Yoghurt whey is potently used as an antibacterial agent in the manufacture of natural solid soap.
Peningkatan Kemampuan Bahasa Inggris Santriwati di Pondok Pesantren Al-Madienah Jombang Noviana, Iin; Sari, Desy Siswi Anjar; Susilowati, Ari
DEDIKASI SAINTEK Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2023): Agustus 2023
Publisher : Al-Hijrah Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58545/djpm.v2i2.181

Abstract

Bahasa Indonesia merupakan Bahasa persatuan, sedangkan Bahasa Inggris Menjadi Bahasa Asing. Bahasa Inggris adalah Bahasa Internasional yang digunakan untuk komunikasi, memahami buku ilmiah yang ditulis dalam Bahasa Asing, Jurnal, atau Bahasa Pada website di era digital saat ini Tujuan dari Pengabdian kepada Masyarakat ini adalah untuk meningkatkan kemampuan Bahasa Inggris santriwati Pondok Pesantren Al Madienah Jombang. Kegiatan pengabdian menggunakan pendekatan partisipatif yang memberikan peran aktif peserta selama proses dan pelaksanaan kegiatan ini dengan memahami Bahasa Inggris dan mampu berkomunikasi aktif menggunakan Bahasa Inggris. Kegiatan ini dilaksanakan di Pondok Pesantren Al Madienah Denanyar, Kabupaten Jombang dan dihadiri oleh 100 santriwati.  Paserta sangat antusias dengan jalan nya diskusi dan tanya jawab. yang diselenggarakan oleh tim pelaksana. Santriwati diwajibkan untuk menggunakan Bahasa Inggris sebagai komunikasi sehari - hari, sehingga komunikasi dan diskusi berjalan lancar. Santriwati mendapatkan pengalaman baru dalam pemahaman Bahasa Inggris. Kewajiban untuk berbicara Bahasa Inggris membantu proses pelaksanaan pengabdian masyarakat, sehingga para santriwati mampu untuk berkomunikasi menggunakan Bahasa Inggris.
Asosiasi Variasi Genotipe PIT1|HinfI pada Tampilan Produksi Susu Sapi Perah Friesian Holstein Indonesia Prastowo, Sigit; Saviera, Shavya Sarah; Vanessa, Rebecca; Pambuko, Galih; Susilowati, Ari; Sutarno, Sutarno
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (795.939 KB) | DOI: 10.33772/jitro.v9i1.17595

Abstract

ABSTRAK Gen Pituitary Specific Transcription Factor 1 (PIT1) adalah gen yang mengatur kelenjar pituitari dan merupakan regulatory factor hormon pertumbuhan serta hormon lain yang terkait dengan produksi susu. Oleh karena itu, penelitian ini bertujuan untuk mengasosiasikan variasi genotipe PIT1 dengan produksi susu sapi Friesian Holstein (FH) di Indonesia. Sebanyak 42 ekor sapi dipilih sebagai sumber DNA. Variasi genotipe ditentukan dengan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) menggunakan enzim restriksi HinfI. Data produksi susu diambil pada laktasi 1 dan 2 yang distandardisasi ke catatan produksi 305 hari. Sebanyak 3 variasi genotipe terdeteksi yaitu AA, AB, dan BB dengan frekuensi masing-masing 0,21; 0,26; dan 0,52. Pada penelitian ini diperoleh frekuensi alel A sebesar 0,35 dan B sebesar 0,65, dan populasi berada pada kondisi setimbang (p<0,05). Genotipe BB pada penelitian ini memproduksi susu lebih tinggi (p>0,05) dibanding AA dan AB pada laktasi 1, namun pada laktasi 2 antar genotipe tidak berbeda nyata. Berdasarkan hasil penelitian maka disimpulkan bahwa variasi genotipe gen PIT1 memiliki potensi untuk digunakan sebagai penanda genetik kemampuan produksi susu sapi FH di Indonesia.Kata Kunci: asosiasi, produksi susu, sapi FH Indonesia, variasi genotip gen PIT1.Association of PIT1|HinfI Genotype Variation on Milk Production of Indonesian Friesian Holstein Dairy Cattle ABSTRACT Pituitary Specific Transcription Factor 1 or known as PIT1, is a gene which function to control the pituitary gland and act as regulatory factor for growth hormone and other hormones related to milk production. The current study was aimed to associate the variant of PIT1 genotype on milk production in Indonesian Friesian Holstein. In total, 42 dairy cows were sampled for DNA source. Phenotypic data of milk yielded from first and second lactation were recorded then standardized to 305 days. To scan PIT1 genotype variation, PCR-RFLP was employed using HinfI restriction enzyme. Three types of PIT1 genotype were found, namely AA, AB, and BB; its frequencies were 0.21; 0.26; and 0.52, respectively. The allele frequencies of allele A and B were 0.35 and 0.65, and the population is in equilibrium condition (p<0.05). Significant associations (p<0.05) were found between genotypes in lactation 1. In this study, BB genotype yielded highest milk compared to other genotypes in lactation 1, but no differences among genotypes were observed in lactation 2. In a conclusion, the variation of PIT1 genotype in Indonesian Friesian Holstein shows potential use for genetic marker to improve milk production in this study.Keywords: association, milk yield, Indonesian Friesian Holstein, PIT1 genotype variation
Antibacterial Production by Endophytic Bacteria from Catharanthus roseus in East Timor Against Methicillin-Resistant Staphylococcus aureus  Missa, Hildegardis; Ndukang, Sardina; Djalo, Aloysus; Nau, Getrudis W.; Susilowati, Ari; Baunsele, Anselmus Boy; Santos, Americo Dos
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.7675

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) refers to a group of bacteria that cause infections and present a challenge in treatment due to their resistance to several antibiotics. Therefore, research is needed to discover endophytic bacteria from Catharatus roseus capable of producing new antibiotics. This study aims to identify endophytic bacteria from C. roseus plants originating from Timor Island, as producers of effective antibacterial compounds against MRSA. Antibacterial activity tests were conducted on two types of test bacteria, namely MRSA and Escherichia coli. DNA extraction was performed using the PrestoTM Mini dDNA kit Bacteria, and amplification of the 16S rRNA gene was carried out. The research found bacterial isolates showing morphological similarities to Bacillus sp. Screening results indicated that four bacterial isolates exhibited high potential antibacterial activity against MRSA, as evidenced by the formation of inhibition zones with diameters of approximately 25 mm-35 mm. Amplification of the four endophytic bacterial isolates from C. roseus identified them as Paenibacillae FaCH2, Bacillae BoCH3, Aneurinibacillae BoCH5, Aneurinibacillae BiCH8, which are new species based on 16S rRNA gene similarity of less than 97%. In conclusion, endophytic bacteria from C. roseus producing antibacterial compounds against MRSA have been successfully identified. This is beneficial to society because the antibacterial compounds produced can serve as a basis for developing new drugs that are effective against MRSA infections, considering the increasing antibiotic resistance against MRSA.
Co-Authors Agung Budiharjo Aldila, Farah Anselmus Boy Baunsele ARTINI PANGASTUTI Chuanata, Wilsen Dewi, Citra Praba Yunita Dhiah Novalina Djalo, Aloysus Dwi Liliek Kusindarta Edi Purwanto Getrudis Wilhelmina Nau Greiner, Ralf Gunawan Hidayati, Muslihah Nur Hidayatullah, Muhammad Eka Hildegardis Missa Mkumbe, Baraka Stewart Mohammad Masykuri Nastiti Wijayanti Ndukang, Sardina Noviana, I'in Noviana, Iin Pambuko, Galih Pangestika, Intan Widya Rahmawati, Sri Indah Rakhmawati, Anik Ratna Dewi Setyowati RATNA SETYANINGSIH Rosmaharani, Shanti Sajidan Sajidan Santos, Americo dos Sari, Desy Siswi Anjar Sari, Siti Lusi Arum Saviera, Shavya Sarah Sigit PRASTOWO Slamet Widiyanto Solikah Ana Estikomah Sri Setyowati Sugiyarto Sugiyarto Suranto Suranto Suranto Sutarno Sutarno Travis, Justin M. J. Vanessa, Rebecca Yunus, Ahmad