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All Journal Jurnal Rekayasa Proses Jurnal Ilmiah Farmasi Jurnal Teknosains Indonesian Journal of Biotechnology JURNAL PHARMASCIENCE INDONESIAN JOURNAL OF PHARMACY Pharmascience Majalah Farmaseutik Jurnal Rekayasa Proses Jurnal Rekayasa Proses
Hilda Ismail, Hilda
Faculty Of Pharmacy, Universitas Gadjah Mada, Yogyakartaa, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Education Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
FORMULATION OF NANOCURCUMIN USING LOW VISCOSITY CHITOSAN POLYMER AND ITS CELLULAR UPTAKE STUDY INTO T47D CELLS Chabib, Lutfi; Martien, Ronny; Ismail, Hilda
Indonesian Journal of Pharmacy Vol 23 No 1, 2012
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.121 KB) | DOI: 10.14499/indonesianjpharm23iss1pp27-35

Abstract

Using  of  curcumin  as  anti  cancer  agent  is  restricted  by  its low  solubility,  therefore  it  has  low  bioavability.  This  obstacle  can be  solved  by  the  development  of  curcumin  nanoparticle. Nanoparticle  technology  has  been  started  to  be  developed  as  an alternative  solution to  improve drug  delivery pofile, especially  for the less bio-available chemical. This study was aimed to develope nanocurcumin  formulation  with  low  viscosity  chitosan  as  the matrix  and  to  study  its  ability  to  be  taken  into  the cells in  vitro. Method  used  in  the  formulation  of  nanocurcumin  in  this  study  is by ionic gelation followed by freeze drying. Entrapment  Efficiency then  assayed,  and  its  stability  was  tested  by  incubating  the formula  into  artificial  intestinal  fluid  (AIF).  Furthermore,  its toxicity  was  evaluated,  also  its  cellular  uptake  ability  into  T47D cell  line.  It  was  found  that  the  Entrapment  Efficiency  in  acetate buffer  at  pH  4  is  higher  than  at  pH  5.  This  formula  also  has  a good  stability  in  AIF.  For  the  cellular  uptake  study  through fluorescence  microscope,  it  was  found  that  the  complex  has  an ability  to  penetrate  cellular  membrane  into  the  cytosol.  The cytotoxicity  study  tell  us  that  the  nanocurcumin  is  non-toxic  to normal  cell line. For  the characterization of the nanoparticles, the average  size  of  this  particle  is  269.8  nm,  its  zeta-potential  is +18.63 mV, with spherical particle morphology. From the result ofthis study, it is concluded that formulation of nanocurcumin using low viscosity chitosan polymer as the matrix has a great potential as an alternative for anticancer therapy.Key words: nanoparticle, curcumin, low viscosity chitosan, T47D cell line. 
Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy Ismail, Hilda; Ciptasari, Ummi H.; Ikhsan, M Arief Nur; Suryani, Fidya; Sismindari, Sismindari; Martien, Ronny; Yuswanto, Ag
Indonesian Journal of Pharmacy Vol 30 No 1, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (988.21 KB) | DOI: 10.14499/indonesianjpharm30iss1pp52

Abstract

Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ-30 was formulated in to nanoparticle using alginate and CaCl2 as cross-linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2% CaCl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl-(dimethylprophilamine) carbodiimide (EDAC).
Co-Authors Adhyatmika Adhyatmika Anggun Feranisa Ari Sudarmanto Arimurni, Dewa Ayu B. S. Ari Sudarmanto Ciptasari, Ummi H. Dwi Titus Indriyawati Endang Lukitaningsih Evi Lande Setiyani Ikhsan, M Arief Nur Indah Tri Nugraha Indah Tri Nur'aini Kurnianto, Rifki Wahyu Lutfi Chabib, Lutfi Martien, Ronny Martien, Ronny Mohammad Fahrurrozi Muhammad Fahrurrozi Pudjono Lukitaningsih Pudjono Pudjono Ratna Asmah Susidarti Ratna Asmah Susidarti Rochmadi Lukitaningsih Rochmadi Rochmadi Ronny Martien Ronny Martien Ronny Martien Ronny Martien S. Sismindari Sismindari, Sismindari Suryani, Fidya Wintari Taurina Yuswanto, Ag