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Journal : JURNAL FARMASIMED (JFM)

IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Rizky, Vincentia Ade; Siregar, Sa’adah; Krisdianilo, Visensius; Rahayu, Asvia; Syafrina Ginting, Suventi; ., Kartini
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.615

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.
GAMBARAN SEL EPITEL PADA LESI PAYUDARA DILABORATORIUM PATOLOGI ANATOMI UPT RSUD DELI SERDANG LUBUK PAKAM ., Kartini; Krisdianilo, Visensius; Sumantri, Bambang; Sidabutar, Rimayani
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.624

Abstract

The breast is an organ that grows as a tissue bulge that is first seen at 7 to 8 weeks after conception. Each breast consists of 12 to 20 tubuloalveolar gland lobules. Each lobule consists of cells consisting of cube epithelial cells and myoepithelial cells that surround the lumen. Lesions in the breast can originate from a connective tissue or from epithelial cells that develop continuously. Some types of epithelial cell traces in breast lesions include fibroadenoma, tumor filoides, papillomas, and papillary carcinoma, and breast carcinoma. This study aims to determine the description of epithelial cells in breast lesions in the Anatomical Pathology Laboratory of UPT Deli Serdang Lubuk Pakam Hospital Period 2018 - 2019. The research was conducted descriptively using the Fine Needle Aspiration Biopsy (FNAB) examination method. The study was conducted using secondary obtained from the medical record of the Anatomical Pathology Laboratory of UPT Deli Serdang Lubuk Pakam Hospital about the description of epithelial cells in breast lesions. The results showed that there were 6 epithelial cell features in breast lesions in the Anatomy Pathology Laboratory of UPT Deli Serdang Hospital. The six epithelial cell features include mammary aberans, mammary adenoma fibrio, mammary dysplasia, mammary multiple fibroadenoma, mammary ductal hyperplasia and mammary carcinoma. From a total of 30 study samples obtained 14 epithelial cells in the form of mammary adenoma fibrio (46.67%). Description of epithelial cells in the form of mammary aberans as many as 7 people (23.33%). For depictions of epithelial cells in the form of mammary carcinoma by 6 people (20%). While the epithelial picture in the form of mammary dysplasia, multiple fibroadenoma mammary and mammary ductal hyperplasia each as much as 1 person (3.33%).