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Sarmin .
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Kadar Mineral Makro dalam Serum Kambing Peranakan Ettawa yang Secara Klinis Sehat (SERUM MACROMINERALS CONCENTRATIONS IN CLINICALLY HEALTHY ETTAWA CROSSBRED GOATS) Irkham Widiyono; Sarmin .
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to evaluate the serum macrominerals status in clinically healthy Ettawa crossbred goatshave been carried out in order to obtain the baseline data for monitoring health condition. A total of 60Ettawa crossbred goats were used in the study. The demographic characteristics of the animals groupwere: (i) young male and female goats (5-8 months); (ii) adult male and female goats (1.5 – 4 years); (iii)pregnant, and (iv) lactating animals. The serum macrominerals concentrations were within the referencestatus of other small ruminant species. The mean calcium (Ca), potassium (K), and chloride (Cl)concentrations were not significantly difference (P>0.05) between the animals groups. The Na concentrationof adult female goats was 139.33 ± 1.94mmol/L and was significantly lower compared to the animals inthe other groups (P<0.05). The inorganic phosphor (Pi) concentrations in pregnant and lactating animalswere significantly lower (P<0.05) compared to that in young, adult, male and female goats. The macromineralsconcentration in serum was influence by the physiological status of the animals.
Respons Metabolik terhadap Pembatasan Asupan Pakan pada Kambing Peranakan Ettawa (METABOLIC RESPONSES TO FOOD RESTRICTION IN ETTAWA CROSSBRED GOATS) Irkham Widiyono; Sarmin .; Bambang Suwignyo
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The study aim of this  was to evaluate the effects of food restriction on metabolic aspects (bloodchemistry parameters) in Ettawa crossbred goats. Four adult male and clinically healthy Ettawa crossbredgoats were used in this study. The animals were kept in individual cage for 12 weeks. Animal feed consistedof aerial part of peanut plant and concentrate at a ratio of 60:40. The experiment was devided into 2phases of feeding periods, fullfeeding (ad libitum  feeding and restricted feeding. After the adaptationperiod for 4 weeks (week 1-4), the animals were fed ad libitum for 4 weeks (week 5-8), and then continuedwith 50% of the ad libitum feed intake during the following 4 weeks restricted feeding period (week 9-12).During the experiment, drinking water was supplied ad libitum. Body weight were examined and bloodsamples were collected at the end of the fullfeeding and restricted feeding periods for blood chemistryanalyses. Statistical differences were determined by paired t-Test at probability level of 0.05. This studyshowed that feed restriction up to about 50% of ad libitum (80% of maintenance) feed intake resulted inchanges of blood chemistry parameters. At the end of restricted feeding period, the elevated concentrationof serum inorganic phosphate, creatinine, blood urea nitrogen, and cholesterine were observed and reducedconcentration of glucose, calcium, and triglyceride were observed. However no change was observed on  levelof arterial blood pH and the concentration of serum natrium, potassium, chloride, and protein. In conclusion,nutritional status has significant effect on blood chemistry parameters of Ettawa crossbred goats. Seruminorganic phosphate, calcium, creatinine, blood urea nitrogen, cholesterine, glucose, and triglycerideconcentration could provide the more reliable information for assessment of the nutritional status ofEttawa crossbred goat.
Profil Saraf Nitrergik Sekum Ayam Pedaging yang Diinfeksi Eimeria tenella (THE PROFILE OF NEORAL NITRERGIC IN THE COECUM OF BROILER INFECTED WITH Emeria tenella) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin .; Claude Mona Airin
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Neurotransmitter found in the enteric nervous system that plays an important role in a variety ofenteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to bedependent on the manner by which inflammation is caused. This study was performed to determine theprofile of neural nitrergic with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d)staining in the cecum of broiler infected by Eimeria tenella (E. tenella). Thirty six health free of coccidiosisbroiler one-day old chickens (DOC) used in this study. All of broiler adapted for 7 days with food and drinkgiven ad libitum then divided into three groups, each group consisted of 12 broilers. The 1st group wascontrol only given orally 1.0 ml of distilled water/ heads , while 2nd group was infected with a single dose of5x103 oocysts/ head orally, and 3rd group was infected with a single dose of 2x104 oocysts/ head orally. Onday 7 post infection, all of chickens were fasted for 12 hours then were euthanized and cecum was taken.Lesion score of cecum was assessed. Furthermore, tissue of the coecum was prepared for hisochemicalusing NADPH-d staining to determine morphology, and morhometric of nitrergic neurons. The resultshown that cecum lesion score of group I is 0, group II is +2, group III is +3. Neuron density in the cecum ofgroup I is 2794.96+4.65 neuron/cm2, group II is 4930.36+63.73 neuron/cm2 and group III is 7892.31+44.97 neuron/cm2. Neuron density of group II and III increased signifcantly (p<001) than group I. Nitrergic neurondesity of group III increased significantly (p<0.05) than that of group II. It was concluded that the infectionof E.tenella led to increase the number of neutrl mienteric nitrergic of the cecum. The higher lesion score ofcecum led to increase the number of mienteric nitrergic neuron.