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IDENTIFIKASI SENYAWA AKTIF ANTIFEEDANT DARI EKSTRAK DAUN PANGI (Pangium Sp) DAN UJI AKTIVITASNYA TERHADAP ULAT KUBIS (Plutella Xylostella) Ida Bagus Putra Mahardika; Ni Made Puspawati; Ida Ayu Gede Widihati
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.236 KB)

Abstract

Leaves of pangi (Pangium edule) are known to have many benefits, including antifeedant activity. 500 g of pangi leaf powder was extracted by maceration method using n-hexane extract yieled 4.51 g of dark green extract. This extracts showed high antifeedant activity (47.23%) against Plutella Xylostella at a concentration of 0.1% (w / v). Separation of 2 g of n-hexane extract by chromatography column using n-hexane: ethyl acetate: chloroform (6:2,5:1,5) as mobile phase and silica gel 60 as stationary phase produced 6 groups of fractions.  Fractions 3 reveiled the highest antifeedant activity that is 52.15%, 69.88%, and 91.66% respectively at a concentration of 10 ppm, 100 ppm, and 1000 ppm. This active fraction which was considered relatively pure following purity test on TLC was then identified using GC-MS spectrometre. GC-MS spectra of antifeedant active compound contained at least 11 compounds with 8 compounds successfully identified as ? – pinene, benzene trimethyl, acetic acid triflouro-tetradecyl ester, nonadecene, 13-Hexyloxacyclotridec-10-en-2-one, phytol, 3-eicosene, , benzenedicarboxylic acid diisooctyl este.
Transesterification of Used Cooking Oil Using CaO/MCM-41 Catalyst Synthesized from Lapindo Mud by Sonochemical Method Ida Bagus Putra Mahardika; Wega Trisunaryanti; Triyono Triyono; Dwi Putra Wijaya; Kumala Dewi
Indonesian Journal of Chemistry Vol 17, No 3 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.303 KB) | DOI: 10.22146/ijc.26561

Abstract

Transesterification of waste cooking oil using CaO/MCM-41 synthesized from Lapindo mud by the sonochemical method has been carried out. The silica was separated from the mud by reflux method used 6 M HCl and 6 M NaOH. The silica was then analyzed by XRF and used as silica source in MCM-41 synthesis. The synthesis of MCM-41 was carried out by the sonochemical method, then analyzed by XRD, Infrared spectrophotometer, SAA, and TEM. The Ca2+ was loaded onto the MCM-41 by wet impregnation method under variation of the Ca2+ content of 1.15, 1.29, 2.39, and 3.25 wt.% analyzed by ICP produced CaO(1), CaO(2), CaO(3), and CaO(4)/MCM-41 catalyst respectively. Transesterification of used cooking oil was carried out under methanol/oil mole ratio of 15/1, the temperature of 55, 65 and 75 °C, and catalyst/oil weight ratio of 5/100, 10/100 and 15/100 for 2 h by reflux method. The XRD analysis of the MCM-41 showed a characteristic peak at 2θ = 2-5°. The MCM-41 has a specific surface area of 1290 m2/g and pore diameter of 3.4 nm. The TEM images of MCM-41 showed ordered pore distribution with a hexagonal shape. The highest conversion of methyl ester was 78.17 wt.% obtained under the reaction conditions at 65 °C and catalyst/oil weight ratio of 15/100 using the CaO(4)/MCM-41. The lifetime CaO(4)/MCM-41 catalyst was 9.8 h.
AKTIVITAS ANTIOKSIDAN DAN PROFIL FITOKIMIA DARI EKSTRAK DAUN PACAR AIR (Impatiens balsamina L.) DENGAN VARIASI PELARUT Mahardika, Ida Bagus Putra; nugraha, iwan saka; Yuliana, ni luh Diah
JFM (Jurnal Farmasi Malahayati) Vol 8, No 2 (2025)
Publisher : Jurnal Farmasi Malahayati (JFM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jfm.v8i2.20737

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh variasi pelarut terhadap aktivitas antioksidan ekstrak daun pacar air (Impatiens balsamina L.) dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Ekstraksi dilakukan dengan metode maserasi menggunakan tiga jenis pelarut, yaitu etanol 96%, n-heksana, dan aquadest. Hasil rendemen menunjukkan pelarut etanol 96% memberikan hasil tertinggi sebesar 17,64%, diikuti aquadest 13,13% dan n-heksana 9,45%. Skrining fitokimia terhadap ekstrak etanol menunjukkan adanya senyawa alkaloid, flavonoid, tanin, saponin, steroid dan terpenoid. Pengujian aktivitas antioksidan dilakukan pada lima konsentrasi (60, 70, 80, 90, dan 100 ppm) menggunakan spektrofotometer UV-Vis pada panjang gelombang 515 nm. Hasil penelitian menunjukkan nilai IC₅₀ ekstrak etanol 96% sebesar 4,06 ppm (kategori sangat kuat), aquadest sebesar 6,24 ppm (sangat kuat), dan n-heksana sebesar 10,53 ppm (sangat kuat). Pemberian variasi pelarut berpengaruh signifikan terhadap aktivitas antioksidan ekstrak daun pacar air, dan pelarut etanol 96% merupakan pelarut paling efektif dalam mengekstrak senyawa antioksidan. 
Analisis Bibliometrik Formulasi Poliherbal sebagai Afrodisiak: Tinjauan Tren Penelitian Terkini Kartika, I Gusti Agung Ayu; Nugraha, Iwan Saka; Mahardika, Ida Bagus Putra; Suryani, Suryani
Jurnal Ilmiah Medicamento Vol 11 No 2 (2025): Jurnal Ilmiah Medicamento (In progress)
Publisher : Fakultas Farmasi Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36733/medicamento.v11i2.11418

Abstract

In recent years, the therapeutic potential of polyherbal formulations as aphrodisiacs, particularly as natural alternatives to synthetic treatments for sexual health disorders, has garnered significant attention. Polyherbal formulations are believed to enhance efficacy through synergistic effects, making them a preferred option in both traditional and modern therapeutic practices. This review aims to provide a comprehensive analysis of research trends relating to the use of polyherbal formulations for aphrodisiac purposes. A bibliometric analysis was conducted using the Scopus database, extracting relevant articles with the keywords 'Aphrodisiac AND Polyherbal OR Combination.' From an initial pool of 88 articles, 16 were selected for in-depth analysis. VOSviewer software was used to visualize collaboration networks, co-authorship patterns, and keyword trends, highlighting the increasing research interest in polyherbal formulations for sexual health enhancement. The narrative review supplements these findings by exploring study methodologies, sources of herbal ingredients, formulation compositions, and commonly used herbs. Results indicate that while polyherbal aphrodisiac formulations show promising therapeutic potential, research in this field remains fragmented, with limited clinical trials evaluating safety and efficacy. The most frequently studied herbs include Tribulus terrestris, Mucuna pruriens, and Withania somnifera, known for their effects on testosterone levels, vascular function, and libido enhancement. Future studies should focus on clinical validation, standardization, and pharmacokinetic assessments to facilitate their integration into modern medical practice. These findings provide valuable insights for researchers and industries developing polyherbal-based nutraceutical and pharmaceutical products.
Determination of Total Polyphenol Content in Bali Local Red Pomegranate (Punica granatum L.) Peel Extract Using UV-Vis Spectrophotometry Kumara Dewi, Ni Wayan Rika; Mahardika, Ida Bagus Putra; Swari Santi, Made Dwike; Ugrasena, Putu Yudha
Jurnal Skala Husada : The Journal of Health Vol 22, No 2 (2025): Jurnal Skala Husada (JSH): The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jsh:tjoh.v22i2.4306

Abstract

ABSTRACTPomegranate (Punica granatum L.) peel is a major agro-industrial byproduct rich in bioactive compounds, particularly polyphenols, which have attracted increasing interest for functional food and phytopharmaceutical applications. However, studies specifically analyzing the total polyphenol content of the local Balinese red pomegranate peel are still limited. This study aimed to determine the total polyphenol content and perform phytochemical screening of the red pomegranate peel collected from Bali, Indonesia. The research employed an experimental laboratory design. Red pomegranate peels were collected from Jadi Village, Tabanan Regency, Bali. The peels were dried, powdered, and extracted by maceration using 96% ethanol. Phytochemical screening was performed to detect major secondary metabolites. The total polyphenol content was determined using the Folin-Ciocalteu method with gallic acid as the standard, measured by UV-Vis spectrophotometry at 765 nm. All analyses were conducted in triplicate. The extraction yielded 25.78% of viscous ethanol extract. Phytochemical screening confirmed the presence of flavonoids, tannins, saponins, and steroids/triterpenoids. The standard gallic acid calibration curve showed good linearity with a correlation coefficient (R²) of 0.9916. The total polyphenol content was found to be 147.953 mg GAE/g at 1,000 ppm concentration and 34.136 mg GAE/g at 100 ppm. The findings demonstrate that the local Balinese red pomegranate peel contains significant polyphenolic compounds and secondary metabolites with antioxidant potential. This supports its potential for valorization as a source of natural antioxidants for functional food, nutraceutical, or sustainable packaging applications. Further studies are recommended to assess its antioxidant activity and to compare seasonal and varietal variations.Keywords:  Punica granatum L.; polyphenol content; Folin-Ciocalteu; UV-Vis spectrophotometry; phytochemical screening.ABSTRAKKulit delima (Punica granatum L.) adalah produk sampingan agro-industri utama yang kaya akan senyawa bioaktif, terutama polifenol, yang semakin menarik minat untuk aplikasi makanan fungsional dan fitofarmasi. Namun, studi yang secara khusus menganalisis total kandungan polifenol dari kulit buah delima merah Bali lokal masih terbatas. Studi ini bertujuan untuk menentukan kandungan total polifenol dan melakukan skrining fitokimia pada kulit buah delima merah yang dikumpulkan dari Bali, Indonesia. Penelitian ini menggunakan desain laboratorium eksperimental. Kulit delima merah dikumpulkan dari Desa Jadi, Kabupaten Tabanan, Bali. Kulit buah delima dikeringkan, dihaluskan, dan diekstraksi dengan cara perendaman menggunakan etanol 96%. Skrining fitokimia dilakukan untuk mendeteksi metabolit sekunder utama. Kandungan total polifenol ditentukan menggunakan metode Folin-Ciocalteu dengan asam galat sebagai standar, diukur dengan spektrofotometri UV-Vis pada 765 nm. Semua analisis dilakukan dalam triplikat. Ekstraksi menghasilkan 25,78% ekstrak etanol kental. Skrining fitokimia mengkonfirmasi keberadaan flavonoid, tanin, saponin, dan steroid/triterpenoid. Kurva kalibrasi asam galat standar menunjukkan linearitas yang baik dengan koefisien korelasi (R²) sebesar 0,9916. Kandungan total polifenol ditemukan sebesar 147,953 mg GAE/g pada konsentrasi 1.000 ppm dan 34,136 mg GAE/g pada 100 ppm. Temuan tersebut menunjukkan bahwa kulit delima merah Bali lokal mengandung senyawa polifenolik yang signifikan dan metabolit sekunder dengan potensi antioksidan. Ini mendukung potensinya untuk valorizasi sebagai sumber antioksidan alami untuk makanan fungsional, nutraceutical, atau aplikasi kemasan berkelanjutan. Studi lebih lanjut direkomendasikan untuk menilai aktivitas antioksidannya dan membandingkan variasi musiman serta varietas. Kata kunci:Punica granatum L.; kandungan polifenol; Folin-Ciocalteu; spektrofotometri UV-Vis; skrining fitokimia.