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Cryopreservation Effects on Consensus Criteria of Human Mesenchymal Stem Cells: A Review Dewi Hambar Sari; Sri Suciati Ningsih
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Broad range applications of human mesenchymal stem cells (MSCs) neccesitate the best way to preserve MSC. Cryopreservation is the only one applicable methods for MSCs storage, which consists of vitrification and slow freezing cryopreservation. However, there is a lack of standard procedure of cryopreservation. Different freezing steps and cryo-mediums are often used, which may impact on MSCs characteristics. This article aims to evaluate the impact of cryopreservation on consensus criteria of MSCs derived from various tissues, which consists of surface morphology, differentiation capacity, and surface marker expression. Twenty-nine in vitro studies were analyzed, and their results organized according to the cell attributes they asses. This review shows that there are various procedures of slow-freezing cryopreservation. Our findings show that a temperature reduction rate of -1oC / min is the most suitable for MSC cryopreservation, and combination of DMSO and FBS in addition of growth medium is still the most used cryomedium in slow freezing cryopreservation. Whereas, in vitrification, combination of ethylene glycol, Ficoll, and sucrose is used as cryoprotective agents. In conclusion, our results show that although there are variations in slow-freezing cryopreservation, there is no significant effect on MSC either morphology, surface marker expression (positive markers consists of CD105, CD90, CD73; and negative markers consists of CD45, CD34, CD14 or CD11b, CD19, and HLA-DR), or differentiation capability (adipogenic, chondrogenic, and osteogenic). Whereas, vitrification effects could not be clearly defined yet, because of a limited number of studies.Key Words: Cryopreservation, Slow-Freezing, Vitrification, MSC characteristiccs, MSC storage

Sosialisasi Bijak Bermedia Sosial sebagai Upaya Mengantisipasi Stres pada Remaja di Masa Pandemi COVID-19 Dewi Hambar Sari; Ulva Melinia Saputri
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 5 No 3 (2021): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Perkumpulan Dosen Indonesia Semesta (DIS) Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v5i3.475

The Coronavirus Disease 2019 (COVID-19) pandemic has an impact on the physical health aspect and the psychology of the community, especially the emotions to experience stress and anxiety. This psychological impact is not only experienced by groups who are vulnerable to COVID-19 but also teenagers. The information circulating on social media about COVID-19, both factual information or hoaxes, triggers stress and anxiety among teenagers. This community service is carried out to provide education to teenagers on how to be wiser using social media so that they can anticipate anxiety and stress as a result of using social media. This activity was carried out for high school teenagers who are members of the youth organization in Ngudi, Blora, by providing counselling on two subjects, namely 1) the SIFT method to anticipate hoaxes circulating on social media, 2) how to anticipate stress as a result of using social media. The results of this activity are participants' understanding of how to behave and act on information obtained from social media, as well as participants' understanding of ways to anticipate stress during the COVID-19 pandemic. In general, the participants were enthusiastic about participating in this activity and get benefits.

Critical Factors Affecting the Participation in COVID-19 Vaccination Program in Residency of Surakarta: The Extended Theory of Planned Behaviour Bayu Sindhu Raharja; Dewi Hambar Sari
Journal of Theoretical and Applied Management (Jurnal Manajemen Teori dan Terapan) Vol. 15 No. 2 (2022)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmtt.v15i2.36335

Objective: This study evaluates the critical factors affecting COVID-19 vaccine acceptance in Indonesia using a proposed extended theory of planned behaviour (extended-TPB). Design/Methods/Approach: We conducted an online survey (N = 222 respondents) using a valid and reliable questionnaire to evaluate people's intention to take the COVID-19 vaccination. A structural equation modeling was performed to demonstrate the impact of religious acceptance (RA), vaccine safety (SF), trust (TS), perceived behavioural control (PBC), attitude towards vaccination (ATT), and subjective norms (SN), on intention (IN) to accept COVID-19 vaccine. Findings: The results show positive relationships of all hypotheses except the correlation between RA and TS. TS becomes a positive determinant of ATT, SN, PBC, and INT, and TS is influenced by SF but not by RA. TS is the key determinant factor of intention to uptake the COVID-19 vaccine. Originality: This study used a proposed extended TPB by adding trust, religious acceptance, and safety as additional components of TPB to predict the intention to participate in COVID-19 vaccination. This study is essential because the vaccine is believed to be the best strategy to deal with virus-related diseases, such as COVID-19. However, there is a lack of information on the multifactor effects of Indonesian people's acceptance of vaccination, especially among Surakarta residents. Practical/Policy implication: This research can be a recommendation for the Indonesian government to elevate public intention to take the COVID-19 vaccine, especially by implementing policies that accentuate people's trust by confirming and publishing information about the vaccine safety.

Pendampingan Sosialisasi Cemaran Khamr dan Alkohol Dalam Makanan Sebagai Persiapan Menghadapi Sertifikasi Halal Dewi Hambar Sari; Nur Tanfidiyah
Dimas: Jurnal Pemikiran Agama untuk Pemberdayaan Vol 22, No 2 (2022)
Publisher : LP2M of Institute for Research and Community Services - UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/dms.2022.222.10838

One of the food-product halalness indicator is the absence of alcohol. In this case, traders as business actors must know about the contamination of khamr and alcohol in food ingredients that are traded so as not to endanger themselves and consumersas a condition when applying for halal certification. Because the content of khamr and alcohol besides endangering health, the Qur'an also clearly forbids them.Therefore this community service aims to increase the knowledge of shop owners around IAIN Surakarta regarding the contamination of khamr and alcohol in food as an effort to prepare for halal certification. This activity is carried out using the socialization method, namely by giving lectures and questions and answers to 10 food stall owners. Activity evaluation is carried out by providing a pre-test and post-test on the topic presented. From this community service activity, there was an increase in knowledge which could be seen from the pre-test and post-test scores, which were respectively 36 and 86. Specifically, the increase in knowledge about the definition of alcohol and khamr was 50%, the rules of the beverage trade. alcohol in Indonesia is 50%, types of alcoholic drinks are 60%, and the law of alcohol and khamr in Islam is 30%.

Penggunaan Koagulan Ganda dan Koagulan – Oksidator dalam Pengolahan Limbah Cair Industri Batik Dewi Hambar Sari
EcoNews Vol 4 No 2 (2021): EcoNews
Publisher : Program Studi Ilmu Lingkungan, Program Pascasarjana Universitas Riau

Batik wastewater contains various chemical compounds, such as dyes, synthetic compounds, heavy metals, and organic compounds that can harm the environment. Therefore, proper waste management is required. The use of alum as coagulant in textile wastewater treatment is commonly used, but it is not effective enough to remove wastewater colour and reduce COD. This study aims to modify alum-based batik wastewater treatment using (i)double coagulant by combining alum - FeSO4; and (ii)coagulant-oxidizing agent with combination of alum – chlorine. This experimental study consisted of two stages, the first experiment aimed to determine the optimum composition dose of alum – FeSO4 and alum – chlorine in depositing waste pollutants, reducing the colour and pH of the effluent; the second experiment aims to determine the effect of alum - FeSO4 and alum - chlorine at the optimum dose in reducing the COD. The results showed that alum–chlorine in various compositions reduced colour ten times and precipitated pollutants two times better than alum-FeSO4. The optimum concentration ratio of alum:chlorine is 1:1 (1.2 g/L alum : 1.2 g/L chlorine). This dose reduced the pH to 8; depositing 0.385 g pollutants; removed 99.57% wastewater colour; and decreased COD from 686.9 mg/L to 235.53 mg/L

PENDAMPINGAN PEMANFAATAN MAGGOT BSF DAN IMPLEMENTASI RECIRCULATING AQUACULTURE SYSTEM DALAM BUDIDYA LELE Dewi Hambar Sari; Ronnawan Juniatmoko; Dwi Purbowati
Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Vol 10 No 2 (2023): Mei
Publisher : Lembaga Penelitian, Penerbitan dan Pengabdian Masyarakat (LP3M) UNSIQ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32699/ppkm.v10i2.4538

Artikel ini melaporkan kegiatan pengabdian kepada masyarakat yang yang bertujuan meningkatkan pengetahuan dan keterampilan peserta dalam memanfaatkan maggot black soldier flies (BSF) dan implementasi recirculating aquaculture system (RAS) untuk meningkatkan efektivitas dan efisiensi proses budidaya lele bagi kelompok budidaya lele di Desa Mojo. Pengabdian dilakukan melalui 2 kegiatan, 1) pendekatan community based research (CBR) dengan melakukan 3 variasi budidaya lele, yaitu T0 (pakan lele komersial, tanpa RAS), T1 (pakan lele komersial + maggot BSF, tanpa RAS), dan T2 (pakan lele komersial + maggot BSF, dengan RAS); 2) Diseminasi hasil CBR. Hasil menunjukkan bahwa budidaya lele dengan maggot BSF + pakan lele komersial yang dikombinasikan dengan implementasi RAS mampu menurunkan biaya pakan dan efektif dalam menjaga kualitas air budidaya. Melalui kegiatan ini, peserta memiliki pengetahuan dan keterampilan awal dalam memanfaatkan maggot BSF dan RAS dalam budidaya lele.Selain itu, pada kegiatan pelatihan dan diseminasi hasil, peserta terlihat antusias mengikuti kegiatan. This article reports community service activities aimed to increase participants’ knowledge and skills in utilizing the BSF larvae and implementing the recirculating aquaculture system (RAS) to increase the effectiveness and efficiency of the catfish farming process for the catfish farming group in Mojo Village. We carried out two main activities, 1) a community-based research (CBR) activity by conducting three variations of catfish farming, namely T0 (commercial catfish feed, without RAS), T1 (commercial catfish feed + BSF larvae, without RAS), and T2 (commercial catfish feed + BSF larvae, with RAS); 2) Dissemination of CBR results. The results show that catfish farming with BSF larvae + commercial catfish feed combined with RAS implementation can reduce feed costs and effectively maintain aquaculture water quality. Through this activity, participants acquire initial knowledge and skills in utilizing BSF larvae and implementing RAS in catfish farming. The participants enthusiastically participated in the training activities and dissemination of the results.

Pendampingan Sosialisasi Cemaran Khamr dan Alkohol Dalam Makanan Sebagai Persiapan Menghadapi Sertifikasi Halal Sari, Dewi Hambar; Tanfidiyah, Nur
Dimas: Jurnal Pemikiran Agama untuk Pemberdayaan Vol. 22 No. 2 (2022)
Publisher : LP2M of Institute for Research and Community Services - UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/dms.2022.222.10838

One of the food-product halalness indicator is the absence of alcohol. In this case, traders as business actors must know about the contamination of khamr and alcohol in food ingredients that are traded so as not to endanger themselves and consumersas a condition when applying for halal certification. Because the content of khamr and alcohol besides endangering health, the Qur'an also clearly forbids them.Therefore this community service aims to increase the knowledge of shop owners around IAIN Surakarta regarding the contamination of khamr and alcohol in food as an effort to prepare for halal certification. This activity is carried out using the socialization method, namely by giving lectures and questions and answers to 10 food stall owners. Activity evaluation is carried out by providing a pre-test and post-test on the topic presented. From this community service activity, there was an increase in knowledge which could be seen from the pre-test and post-test scores, which were respectively 36 and 86. Specifically, the increase in knowledge about the definition of alcohol and khamr was 50%, the rules of the beverage trade. alcohol in Indonesia is 50%, types of alcoholic drinks are 60%, and the law of alcohol and khamr in Islam is 30%.

Eksplorasi In Silico Senyawa Fitokimia untuk Penghambatan Protein STAT3 dalam Mengatasi Resistensi Obat pada Kanker Payudara Triple Negatif Sari, Dewi Hambar; Ningsih, Sri Suciati; Islami, Suryadi
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9174

Breast cancer is the most common cancer among women and a major cause of cancer-related death worldwide, including Indonesia. Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer, characterized by high mortality and resistance to standard treatments. Cancer stem cells (CSCs) is known to be a key factor of drug resistance in TNBC, mediated by the signal tranducer and activator of transcription factor 3 (STAT3). This study aims to explore the bioactive compounds from Indonesian’s natural product which potentially target CSCs by inhibiting STAT3. We used in silico methods, consist of literature review, followed by selection of promising compounds, ADMET analysis and selection using Lipinski’s rules, and molecular docking simulation. Our results found curcumin, quercetin, α-mangostin, gingerol, and rocalglamide as the bioactive candidates which target CSCs. ADMET testing showed that only curcumin, quercetin, and gingerol meet the Lipinski’s criteria. The molecular docking results showed that quercetin exhibited the strongest binding affinity (-8.3 kcal/mol) compared to curcumin (-6.9 kcal/mol) and gingerol (-5.9 kcal/mol). The results indicate that quercetin binds effectively at the active site of STAT3. The findings suggest that quercetin has potential as a STAT3 inhibitor. Further research is needed to determine the significant efficacy and potential as an anticancer.

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