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All Journal JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Medika Cendikia (e-Journal)
Eltivitasari, Andita
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Qualitative and Quantitative Analysis of Compounds in Kencur Extract Using TLC and UV-Vis Spectrophotometry Amarta, Fitri Dwi; Haresmita, Perdana Priya; Eltivitasari, Andita
Jurnal Medika Cendikia Vol 11 No 02 (2024): Jurnal Medika Cendikia
Publisher : Karsa Husada Health Institute Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33482/jmc.v11i02.252

Abstract

Kencur (Kaempferia galanga L.) is one of the plants in the Zingiberaceae family (gingers) that is often used by people in Asia, including Indonesia. Kencur is also widely used as a traditional medicine because it contains active compounds that are beneficial for treatment. This research aims to conduct a qualitative and quantitative analysis of secondary metabolites in Kencur extract samples. Qualitative analysis was conducted using thin layer chromatography with three variations of the mobile phase: Chloroform : Methanol (95:5), Chloroform : Methanol : Glacial Acetic Acid (94:5:1), Chloroform : n-Hexane : Methanol (1:1:0.1), and the stationary phase Silica Gel GF254. Meanwhile, the quantitative analysis was conducted using UV-Vis spectrophotometry at a wavelength of 429.5 nm. The research results show that Kencur contains compounds such as tannins, glycosides, and terpenoids. This research also identifies the presence of curcumin, which has antioxidant effects and other benefits. The results indicate that 2 out of 3 experiments showed positive results, with Kencur containing curcumin at levels of 0.4593% and 0.4393%. The methods used were qualitative methods through thin-layer chromatography and quantitative analysis of curcumin levels in the samples using UV-Vis spectrophotometry.
Jamur Endofit Arthrinium sp., Sumber Potensial Senyawa Obat Review Eltivitasari, Andita; Wahyuono, Subagus; Astuti, Puji
JSFK (Jurnal Sains Farmasi & Klinis) Vol 8 No 3 (2021): J Sains Farm Klin 8(3), Desember 2021
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.8.3.228-241.2021

Abstract

Keberadaan jamur endofit di alam sangat melimpah dan mudah ditemukan pada jaringan tanaman antara lain pada akar, batang, daun, bunga atau biji. Endofit berkoloni di area antar dan intra-sel dari jaringan tanaman tanpa efek merugikan pada tanaman tersebut. Jamur endofit mempunyai kemampuan memproduksi senyawa bioaktif yang dapat sama atau berbeda dari tumbuhan inangnya dan mempunyai efek farmakologis.  Arthrinium sp. merupakan contoh jenis jamur endofit yang keberadaannya melimpah. Arthrinium sp. diketahui mempunyai kandungan metabolit sekunder yang potensial untuk dikembangkan. Senyawa golongan terpenoid, alkaloid, fenolik dan beberapa golongan lain ditemukan dalam jamur endofit Arthrinium sp. Senyawa yang dihasilkan oleh Arthrinium sp. dilaporkan menunjukkan aktivitas biologis yang beragam yaitu sebagai antimikroba, antioksidan, antikanker dan aktivitas yang lainnya. Tulisan ini merangkum pengetahuan, studi dan laporan mengenai jamur endofit Arthrinium sp., kajian aktivitas biologi serta metabolit sekunder yang dihasilkan yang berpotensi dikembangkan sebagai penemuan obat baru yang berasal dari alam.
Co-Authors Amarta, Fitri Dwi Perdana Priya Haresmita, Perdana Priya Puji Astuti Subagus Wahyuono