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All Journal HAYATI Journal of Biosciences Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal The Journal of Society and Media BioEksakta : Jurnal Ilmiah Biologi Unsoed Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Advance Sustainable Science, Engineering and Technology (ASSET)
Agus Nuryanto
Fac. of Biology Jenderal Soedirman University Purwokerto
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Social Sciences Veterinary

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Journal : Advance Sustainable Science, Engineering and Technology (ASSET)

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Molecular Identification of Color Variants of Procambarus clarkii Using the COI Gene for Taxonomic Validation Bhagawati, Dian; Husein Sastranegara, Muhammad; Agus Nuryanto; Anastasia Endang Pulungsari; Elly Tuti Winarni; Atang; Hanan Hassan Alsheikh Mahmoud
Advance Sustainable Science Engineering and Technology Vol. 7 No. 2 (2025): February-April
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/hcaz9264

Abstract

This study addresses taxonomic uncertainty surrounding color variants of Procambarus clarkii in aquaculture and conservation contexts. We investigated whether commercially significant color morphs represent distinct subspecies or phenotypic variations of a single species. Using the cytochrome c oxidase subunit I (COI) gene as a molecular marker, we analyzed four color morphs (blue, white, orange, and brown) from aquaculture facilities in Banyumas Regency. DNA extraction, PCR amplification, and sequencing were performed on 20 specimens. Results showed high sequence homology (98.7-99.8%) across all variants, confirming they belong to a single species. Genetic distance analysis revealed minimal divergence (0.2-1.3%), insufficient for subspecies classification. Phylogenetic reconstruction demonstrated specimens clustered by genetic similarity rather than color or geographic origin, indicating coloration results from genetic mutations rather than environmental adaptations. This COI-based approach provides a molecular framework for taxonomic classification of P. clarkii varieties, with implications for breeding programs, variety certification, and management of this economically important yet potentially invasive species.
Co-Authors Atang D.P.H. Handayani Dedi Soedharma Diana Retna Utarini Suci Rahayu, Diana Retna Utarini Suci DIETMAR BLOHM Dyah Purry Elly Tuti Winarni Farida Nur Rachmawati Hanan Hassan Alsheikh Mahmoud Husein Sastranegara, Muhammad Pulungsari, Anastasia Endang TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN