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All Journal JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Annales Bogorienses Berita Biologi
Dwi Agustiyani
Lembaga Ilmu Pengetahuan Indonesia, Cibinong
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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PENAPISAN DAN KARAKTERISASI RHIZOBAKTERIA SERTA UJI AKTIVITASNYA DALAM MENDUKUNG PERKECAMBAHAN DAN PERTUMBUHAN BENIH JAGUNG (ZEA MAYS L.) Agustiyani, Dwi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2890

Abstract

ABSTRACTPlant Growth Promoting Rhizobacteria (PGPR) are a group of bacteria known to influence plant growth by direct or indirect mechanisms. In search of efficient PGPR which potential as biofertilizers, a total of 26 isolates of rhizobacteria were isolated from various plant rhizosphere. These 26 isolates were screened through in vitro methods for PGPR properties like phosphate solubilization, siderophore, IAA and amonia production, and catalase activity. The result revealed that 9 isolates showed positive activities for phosphate solubilization 17 isolates for IAA production 18 isolates for siderophores 19 isolates for ammonia production and all of the isolates have catalase activities . Only four isolates (Az.KT.CSC, Az.D.8B, Az.D.8A and Az.Lo.10B) exhibited multiple plant growth promoting traits viz., phosphate solubilization, siderophore, IAA, amonia production, and catalase activity. Ten isolates that have different characters were further investigated for quantitative analysis of IAA production, HCN production and its effect on germination and seedling growth of Zea mays. The range of IAA production was 3,12? 134,27 ppm, among ten isolates, Az.D.8B isolate produced the highest IAA (134,27 ppm). Production of HCN was detected in eight isolates. Four isolates positively affected the germination of Zea mays seeds. Highest root elongation was recorded when seeds were treated with Az.Lo.5 isolate. Whereas, the highest chlorophyl content and plant high were recorded when seeds were treated with Az.B.8B isolate.Keywords: N-fixing bacteria, PGPR, IAA, siderophore, catalase
OKSIDASI NITRIT OLEH BAKTERI HETEROTROFIK PADA KONDISI AEROBIK Agustiyani, Dwi; Kayadoe, Ruly Marthina; Imamuddin, Hartati
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3164

Abstract

ABSTRACTNitrite Oxidation by Heterotrophic Bacteria Under Aerobic Condition. The nitrite transformingactivities of heterotrophic bacteria from isolates from agriculture soil, Lampung were studiedunder aerobic conditions. Among the 9 bacterial isolates tested, almost all are reported haveability to consume of nitrite, but none of the bacterial isolates formed significant nitrate in themedium. NOB H1 (Bacillus licheniformis), is denitrification-negative, consumed 16. 4 mg/L ofnitrites with the accumulation of 4.45 mg/L nitrates. While, NOB H8 (Pseudomonas sp.) isdenitrification-positive, consumed 49. 64 mg/L of nitrite with the accumulation of 3.34 mg/Lnitrates. Nitrite oxidations of both isolates NOB H1 and NOB H8 took place during stationeryphase to the dead phase. Growth pattern of both isolates NOB H1 and NOB H8 were sigmoidwith generation time of 1.69 and 2.19 hour, respectivelyKey words: heterotrophicbacteria; nitrite oxidation; denitrification
KARAKTER PERTUMBUHAN DAN AKTIVITAS NITRIFIKASI KULTUR MIKROBA N-SW Agustiyani, Dwi; Imamuddin, Hartati; Haryanto, Tanto
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3211

Abstract

ABSTRACTThe growth character and nitrification activity of nitrifying cultures (N-Sw). Theculture of nitrifiers (N-Sw) was obtained from acclimated sludge of sawit palm oil industrywastewater. The growth and nitrification activity of those cultures were investigated.The result shows that the growth and nitrification activity attained optimum at pH 7-8,and temperature of 30 0C. The culture of nitrifiers was still growing at pH 5, but thenitrification activity was not detected. The growth of nitrifiers and the nitrification activitywas inhibited at 40 0C. The ammonium conversion rate reached 0,088-0,090 mg NNH4+/L/hour/g biomass. The rate of ammonium conversion in the bioreactor increasedto 0.630 mg N-NH4+/L/hour/g biomass as the pH maintained at 7.5-8 and dissolvedoxygen at 3-4 mg/l O2.Key words: nitrifying culture, ammonium, ammonium convertion rate
Denitrification of Activated Sludge in The Presence of Different Organic Substrates Agustiyani, Dwi; Yamagishi, Takao
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

The effect of organic carbon denitrifying activity was studied in batch reactor. Fom reactors were operated in parallel under anoxic condition in four different donor electrons. which were acetic acid (Reactor A), methanol (Reactor M), phenol (Reactor P), and glucose (Reactor G). The reactors were fed with the artificial waste, which contain 721.8 mg/l NaNO3. The concentration organic carbon added to the reactors were varied from TOD:N ratio of 0.5:1; 1: 1, 1.5:1, to 2:1. The denitrification activity was estimated by measuring the reduction rate of nitrogenous oxide and N2O gas production. The denitrification capacity of adapted-sludge was also investigated, and the rates were estimated from the cumulative N2O (without acetylene inhibition) and N2 gas production. Reduction rate of nitrogenous oxide in all reactors increased during the investigation; lhe increase reduction rate were correlated to the increase of organic carbon concentration. The maximum reduction rate of nitrogenous oxide in Reactor A was higher than those of the others. However, reduction rate in Reactor M was more constant, so that nitrogenous oxides existed in this reactor was removed faster. The highest potential denitrification rate (N20 production) was observed in sludge of Reactor A. However, N2 gas recovery trom nitrate and nitrite transformed by sludge of Reactor M was the highest. Linear correlation between nitrogenous oxide reduction with gas production was observed in Reactor A, M and P, but not in Reactor G.
POTENSI RHIZOBAKTERI DALAM MENDUKUNG PERTUMBUHAN STEK BATANG SINGKONG (Manihot esculenta Crantz) [The Potential of Rhizobacteria in Supporting the Growth of Cassava (Manihot esculenta Crantz ) Stem Cuttings] Nugroho, Agung Adi; Agustiyani, Dwi; Sutisna, Entis; Mulyani, Nani; Dewi, Tirta Kumala; Nditasari, Achirul; Antonius, Sarjiya; Purwaningsih, Sri
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.120

Abstract

Cassava (Manihot esculenta Crantz) is an important food plant in Indonesia, particularly as a carbohydrate source. We require a plan to increase production and quality. The use of Plant Growth Promoting Rhizobacteria (PGPR) in organic fertilizers is one option. The aim of this study is to isolate, characterize, and evaluate potential microbial candidates for cassava greenhouse growth. K solubilizing, P solubilizing, N fixing, IAA production, protease production, ACC-deaminase, and siderophore production were investigated in 15 tuber root rhizobacteria isolates. The bacterial isolates' ability to grow cassava stem cuttings was then assessed. The rhizobacterial inoculation treatments in this study included fifteen single isolates, a combination of isolates, and controls. With three replications, the experimental design was completely randomized. Five P-solubilizing bacterial isolates (PK AL.2.2, PK LAS 4A.4, PK 4.1, PK Al.2.6, PK Kbm 6.1), two protease-producing isolates (Pro LAS 4B.6 and Pro LAS 5A.3), and one IAA-producing isolate (TSB LAS 1A.6) increased wet plant weight by 20-41% as compared to controls. The results of molecular identification of the six excellent isolates were Burkholderia sp. (PK AL.2.2), Paenarthrobacter nicotinovorans (Pro LAS 4B.6), Burkholderia territorii (PK 4.1), Burkholderia cenocepacia (Pro LAS 5A.3), Burkholderia territorii (PK LAS 4A.4), and Kocuria rhizophila (TSB LAS 1A.6).
Co-Authors Achirul Nditasari Agung Adi Nugroho, Agung Adi Dewi, Tirta Kumala Edi Gunawan Entis Sutisna Hartati Imamuddin Haryanto, Tanto Haryanto, Tanto Kayadoe, Ruly Marthina Kayadoe, Ruly Marthina latifah K Darusman Maman Rahmansyah Martono, Budhi Mulyani, Nani Nur Laili Sarjiya Antonius Sri Purwaningsih Supriyati, Dyah Yamagishi, Takao