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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Disease Prevention and Public Health Journal Pharmaciana: Jurnal Kefarmasian Biology, Medicine, & Natural Product Chemistry Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan JURNAL PENGABDI Jaringan Laboratorium Medis Jurnal Pengabdian Dharma Bakti Respati Jurnal Kesehatan Saintika Meditory
Resmi Aini
Poltekkes Bhakti Setya Indonesia, Yogyakarta
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Energy Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences

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Sensitivitas dan Spesifisitas Pemeriksaan Antibodi Human Immunodeficiency Virus (HIV) dengan Perbedaan Metode Imunokromatografi Rapid Test terhadap ECLIA (Electro Chemiluminescence Immuno Assay) Aini, Resmi; Padmarini, Adira; Sulistyo, Andhy
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13620

Abstract

The requirement for HIV reagents using the Immunochromatographic Rapid Test method must comply with the WHO prequalification standards and evaluation by the National Evaluator Agency to ensure their quality and safety; therefore, their sensitivity and specificity were tested against the ECLIA method as the gold standard. To determine the sensitivity and specificity of HIV testing using the Immunochromatographic Rapid Test compared with ECLIA at UDD PMI Yogyakarta City. The study population consisted of all blood donor samples undergoing screening for transfusion-transmissible infections (TTI). Sampling was conducted using the Systematic Random Sampling technique, by determining a random starting point with a Random Number Generator and subsequently selecting samples at fixed intervals until 30 samples were obtained. These samples were then tested using both ECLIA and the Rapid Test. The study design was observational analytic with a cross-sectional approach to evaluate the correlation between Rapid Test results and the ECLIA method as the gold standard, and to calculate sensitivity, specificity, PPV, and NPV accurately. A total of 30 blood donor samples were examined. Based on the ECLIA results, 4 samples were identified as HIV-reactive and 26 samples were non-reactive. The Immunochromatographic Rapid Test detected only 2 of the 4 ECLIA-confirmed reactive cases. The test showed high specificity (100%) but relatively low sensitivity (50%). ECLIA detected 4 (13.3%) reactive and 26 (86.3%) non-reactive samples. The sensitivity of the Immunochromatographic Rapid Test compared to ECLIA was 50%, while the specificity was 100%. These results indicate that the Rapid Test does not meet the requirements stated in the 2018 CPOB guidelines, particularly regarding HIV screening, which requires a specificity of more than 98%.
Karakteristik Pendonor Yang Lolos Seleksi Donor terkait Tekanan Darah Normal dan Hipertensi Derajat 1 di Unit Transfusi Darah Kabupaten Bantul Astarini, Dian Eko; Khunevy, Cindy Khaliza; Aini, Resmi
Jurnal Kesehatan Saintika Meditory Vol 8, No 2 (2025): November 2025
Publisher : STIKES Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jsm.v8i2.3329

Abstract

Pendahuluan: Upaya pelayanan transfusi dengan menggunakan darah pendonor sebagai bahan baku yang bertujuan untuk menjamin ketersedian komponen darah aman, berkualitas dan cukup terus ditingkatkan. Bahan baku darah didapat dari calon pendonor yang sudah dinyatakan lolos pemeriksaan seleksi donor. Syarat seleksi donor salah satunya adalah tekanan darah yang dinilai penting dalam pengolahan komponen. Tujuan Penelitian: Untuk mengetahui gambaran tekanan darah yang lolos seleksi donor di UTD PMI Kabupaten Bantul. Bahan dan Metode: Jenis penelitian menggunakan deskritif kuantitatif dan retrospektif, dengan menggunakan teknik total sampling pada seluruh pendonor lolos seleksi tekanan darah di UTD PMI Kabupaten Bantul tahun 2024. Hasil Penelitian: Dari 11.439 pendonor lolos seleksi tekanan darah menunjukkan tertinggi semua karakteristik adalah pada kelompok tekanan darah normal yaitu  golongan darah O tertinggi yaitu 3.135 (34,8%), kelompok usia terbanyak adalah 25-44 tahun sejumlah 5.420 (47.5%), jenis kelamin dominasi laki laki sejumlah 7.846 (68,7%), dan jenis donasi sukarela paling banyak yaitu 10.064 (87,9%). Kesimpulan: Karakteristik golongan darah tertinggi adalah pada kelompok tekanan darah normal dengan golongan darah O, kelompok usia 25-44 tahun, Jenis kelamin laki laki dan jenis donasi dari pendonor sukarela.Kata kunci : Seleksi donor; tekanan darah; karakteristik pendonor.
Co-Authors Ana Mardiyaningsih Anton Yudhana Astarini, Dian Eko Azimata R, Rudina B. Labito, Rahmin Handayani, Reska Hendra Rohman Ismiyati Ismiyati Jarwati Susiloningsih Khunevy, Cindy Khaliza Nadhifa, Nuha Afra Oktaviyanti, Rina Padmarini, Adira R., Rudina Azimata Rahmatullah, Widia Rina Widiastti, Rina Rina Widiastuti, Rina Sary, Renip Ruwidha Sulistyo, Andhy Sunardi Sunardi