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UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH MARKISA UNGU (Passiflora Edulis sims) TERHADAP Staphylococcus aureus Anabel, Anabel; Denhara Wijaya, Cindy; Lokanata, Shieny
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 6 No. 3 (2020)
Publisher : Universitas Tadulako

Penelitian dilakukan untuk mengetahui efektivitas antibakteri ekstrak kulit buah markisa dalammenghambat pertumbuhan bakteri Staphylococcus aureus penyebab terjadinya infeksi sepertiAngular cheilitis, abses, dan denture stomatitis. Kulit buah markisa ungu dimaserasi,di pecahkankonsentrasi 30%, 50%, 75% dan 100%. Metode uji dengan disc diffusion ( Tes Kirby-Bauer)dilanjutkan uji statistik one way ANOVA Nilai rata- rata zona hambat terendah adalah 12,8 mm danterbesar 16,7 mm. Maka ekstrak kulit buah dari markisa berwarna ungu dengan konsentrasi 100%memiliki efek antibakteri tertinggi terhadap Staphylococcus aureus dan efek antibakteri terendahpada konsentrasi 30%.

Formulation and physical stability analysis of red beetroots (Beta vulgaris l.) effervescent granules Florenly, Florenly; Lokanata, Shieny; Friando, Jovin; Mai, Phuong Thao; Le, Hong Hai; Luzria, Cut Cynthia; Sintapanont, Anusak
Padjadjaran Journal of Dentistry Vol 35, No 2 (2023): July 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol35no2.47031

ABSTRACTIntroduction: Red beetroots (Beta vulgaris L.) are a natural ingredient rich in nutrients and provide various notable health benefits. In dentistry, red beetroots have the potential to act as a plaque-disclosing agent. Previous studies showed that red beetroot preparation in gelform is more effective compared to chewable tablets, but has significant drawbacks in taste. Effervescent granules have the potential to conceal the unpleasant taste of beetroot substances. This study aims to determine the ideal formula and evaluate the physical properties and stability of effervescent granules made from red beetroot extract. Methods: This was an experimental design study with descriptive analysis. The maceration method was used to extract the red beetroot constituents. Fresh fruits were collected, air-dried, and macerated with 96% ethanol twice. The filtrates were then concentrated. The wet granulation method was used to formulate effervescent granules. The samples were grouped into formula 1 (F1), formula 2 (F2), and formula 3 (F3) using 1%, 3%, and 4% red beetroot extract, respectively. All sample groups were analyzed for physical stability, namely: organoleptic, flowability, density, dissolving time, and pH tests. Results: All samples surpassed the sensory evaluation for physical properties. Formula 1 (1%) and formula 2 (3%) models passed all the physical stability tests. Meanwhile, the 4% formula (F3) failed two physical tests. F1 (1%) samples also showed lighter brown color for its lower beetroot extract concentration, indicating under-qualification as dental plaque staining materials. Therefore, 3% concentration (F2) has the best formulation regarding physical properties and stability. Conclusion: Formulas F1 and F2 have the best physical properties and stability by passing all the tests, while Formula 3 failed the organoleptic and flow rate tests.

The effectiveness of natural tooth whitening using herbal substances on coffee-induced tooth discoloration Purba, Member Reni; Tarigan, Gabriel C.J.; Lokanata, Shieny
Buletin Kedokteran & Kesehatan Prima Vol. 4 No. 1 (2025): March
Publisher : Fakultas Kedokteran, Kedokteran Gigi, dan Ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/bkkp.v4i1.6793

Tooth discoloration can be caused by many factors, including food and beverages. One of the drinks that can affect tooth color is coffee. Tooth discoloration can be overcome by teeth whitening procedures, which often use chemicals that can cause tooth sensitivity. Therefore, in this study, researchers are looking for safer alternatives, namely using herbal ingredients such as kepok banana peel and pineapple stem. This study used a laboratory experimental method with a pre-test and post-test only research design with control groups. The sample consisted of 27 central and lateral incisor teeth, which were divided into three treatment groups. The samples were then soaked for 14 days in coffee solution, and then group one was soaked in 100% kepok banana peel extract, group two was soaked in 100% pineapple stem extract, and group three as a control group was soaked in 6% hydrogen peroxide. All samples were soaked for two days in an incubator. The results of the one-way ANOVA test showed that there was a significant color difference (p<0.05) between the three treatment groups. The Post Hoc LSD test showed there was a significant difference between the 6% hydrogen peroxide group and the two extract groups, and also a significant difference between 100% kepok banana peel and 100% pineapple stem extract. Each group has an effect on tooth discoloration. Hydrogen peroxide is indeed the group with the highest tooth color change but has an effect that can make teeth sensitive. The banana peel and pineapple stem extract groups also had a significant and safer effect on teeth. 100% kepok banana peel extract and 100% pineapple stem extract have an effect on tooth discoloration but are not better than hydrogen peroxide. In addition, 100% pineapple stem extract has a better ability in teeth whitening than 100% kepok banana peel extract.

Unveiling the Antibacterial Potential of Arumanis Mango (Mangifera indica L.) Leaf Extract Against Enterococcus faecalis: A Comparative Study with Melinjo (Gnetum gnemon L.) Lokanata, Shieny; Roselyn Avrillia; Marco Luman; Yossye Joyce Magdalena Br Sirait; Daffa Nabilah Panggabean
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1299

Background: Root canal infections, frequently caused by the bacterium Enterococcus faecalis due to its resistance to conventional treatments, present a significant challenge in dental practice. Effective disinfection of the root canal system is crucial for successful endodontic therapy, necessitating the exploration of alternative irrigating solutions. This study aimed to evaluate and compare the in vitro antibacterial efficacy of Arumanis mango (Mangifera indica L.) leaf extract and melinjo (Gnetum gnemon L.) leaf extract against Enterococcus faecalis. Methods: This in vitro experimental study employed the disc diffusion method to assess the antibacterial activity of ethanolic extracts of Arumanis mango and melinjo leaves against Enterococcus faecalis. The extracts were tested at three different concentrations: 25%, 50%, and 75%. Chlorhexidine 0.2% and distilled water served as positive and negative controls, respectively. The diameter of the inhibition zones around the discs was measured after incubation. Phytochemical screening of both extracts was also conducted. Results: The phytochemical screening revealed the presence of flavonoids, alkaloids, and tannins in both Arumanis mango and melinjo leaf extracts. However, the antibacterial activity varied significantly. Melinjo leaf extract did not exhibit any measurable inhibitory effect on Enterococcus faecalis at any of the tested concentrations. In contrast, Arumanis mango leaf extract demonstrated significant antibacterial activity at concentrations of 50% and 75%, with mean inhibition zone diameters of 11.73 ± 0.75 mm and 12.90 ± 0.30 mm, respectively. Statistical analysis using the Kruskal-Wallis test and post-hoc Mann-Whitney U test confirmed a significant difference in the antibacterial activity between the Arumanis mango leaf extract at 50% and 75% concentrations and the melinjo leaf extract at all tested concentrations, as well as the distilled water control. The positive control, chlorhexidine 0.2%, showed the largest inhibition zone (15.10 ± 0.10 mm). Conclusion: The findings of this study indicate that the ethanolic extract of Arumanis mango (Mangifera indica L.) leaves possesses significant in vitro antibacterial activity against Enterococcus faecalis, suggesting its potential as a natural alternative irrigating solution in endodontic treatment. Conversely, under the conditions of this study, melinjo (Gnetum gnemon L.) leaf extract did not demonstrate any antibacterial effect against this specific bacterium. Further research is warranted to explore the clinical efficacy and safety of Arumanis mango leaf extract in root canal disinfection.

Unveiling the Antibacterial Potential of Arumanis Mango (Mangifera indica L.) Leaf Extract Against Enterococcus faecalis: A Comparative Study with Melinjo (Gnetum gnemon L.) Lokanata, Shieny; Roselyn Avrillia; Marco Luman; Yossye Joyce Magdalena Br Sirait; Daffa Nabilah Panggabean
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1299

Background: Root canal infections, frequently caused by the bacterium Enterococcus faecalis due to its resistance to conventional treatments, present a significant challenge in dental practice. Effective disinfection of the root canal system is crucial for successful endodontic therapy, necessitating the exploration of alternative irrigating solutions. This study aimed to evaluate and compare the in vitro antibacterial efficacy of Arumanis mango (Mangifera indica L.) leaf extract and melinjo (Gnetum gnemon L.) leaf extract against Enterococcus faecalis. Methods: This in vitro experimental study employed the disc diffusion method to assess the antibacterial activity of ethanolic extracts of Arumanis mango and melinjo leaves against Enterococcus faecalis. The extracts were tested at three different concentrations: 25%, 50%, and 75%. Chlorhexidine 0.2% and distilled water served as positive and negative controls, respectively. The diameter of the inhibition zones around the discs was measured after incubation. Phytochemical screening of both extracts was also conducted. Results: The phytochemical screening revealed the presence of flavonoids, alkaloids, and tannins in both Arumanis mango and melinjo leaf extracts. However, the antibacterial activity varied significantly. Melinjo leaf extract did not exhibit any measurable inhibitory effect on Enterococcus faecalis at any of the tested concentrations. In contrast, Arumanis mango leaf extract demonstrated significant antibacterial activity at concentrations of 50% and 75%, with mean inhibition zone diameters of 11.73 ± 0.75 mm and 12.90 ± 0.30 mm, respectively. Statistical analysis using the Kruskal-Wallis test and post-hoc Mann-Whitney U test confirmed a significant difference in the antibacterial activity between the Arumanis mango leaf extract at 50% and 75% concentrations and the melinjo leaf extract at all tested concentrations, as well as the distilled water control. The positive control, chlorhexidine 0.2%, showed the largest inhibition zone (15.10 ± 0.10 mm). Conclusion: The findings of this study indicate that the ethanolic extract of Arumanis mango (Mangifera indica L.) leaves possesses significant in vitro antibacterial activity against Enterococcus faecalis, suggesting its potential as a natural alternative irrigating solution in endodontic treatment. Conversely, under the conditions of this study, melinjo (Gnetum gnemon L.) leaf extract did not demonstrate any antibacterial effect against this specific bacterium. Further research is warranted to explore the clinical efficacy and safety of Arumanis mango leaf extract in root canal disinfection.

The Effectiveness of Turmeric Extract (Curcuma domestica Val.) Against Fibroblast Cells in Socket Post Tooth Extraction Lokanata, Shieny; Sendyra Utama; Mellisa Sim
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 4 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i4.490

Background. This study is an exploration to assess the potential of turmeric extract in the induction of fibroblast cell proliferation, where fibroblast cells are important cells in the process of repair and healing of wounds (socket wounds after tooth extraction). The antioxidants found in turmeric extract are believed to have potential in the initiation of growth factors which will then initiate the process of fibroblast cell activation, which in turn will play a role in wound healing. Methods. The research design in this study is an experimental study with a post-test-only approach with a control group design. The research subjects in this study were rats (Rattus norvegicus) Wistar strain, male sex, age 8-12 weeks, and body weight between 150-250 grams. A total of 30 white rats were grouped into 5 groups. Data analysis was carried out with the help of SPSS 25 software. First, a univariate analysis was performed to present the mean number of fibroblast cells and the standard deviation. Furthermore, an analysis was carried out to assess the difference in the mean number of fibroblast cells between groups using the one-way ANOVA test. Results. The results of the one-way ANOVA test showed that there were significant differences between the test groups. Giving 10% and 15% turmeric extract was seen to be more potent in the initiation of the number of fibroblast cells than the treatment group that received povidone-iodine. Conclusion. Turmeric extract (Curcuma domestica Val.) concentration of 15% was the most effective concentration as an effort to increase the number of fibroblast cells after tooth extraction in Wistar rats.

The Effectiveness of Turmeric Extract (Curcuma domestica Val.) Against Fibroblast Cells in Socket Post Tooth Extraction Lokanata, Shieny; Sendyra Utama; Mellisa Sim
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 4 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i4.490

Background. This study is an exploration to assess the potential of turmeric extract in the induction of fibroblast cell proliferation, where fibroblast cells are important cells in the process of repair and healing of wounds (socket wounds after tooth extraction). The antioxidants found in turmeric extract are believed to have potential in the initiation of growth factors which will then initiate the process of fibroblast cell activation, which in turn will play a role in wound healing. Methods. The research design in this study is an experimental study with a post-test-only approach with a control group design. The research subjects in this study were rats (Rattus norvegicus) Wistar strain, male sex, age 8-12 weeks, and body weight between 150-250 grams. A total of 30 white rats were grouped into 5 groups. Data analysis was carried out with the help of SPSS 25 software. First, a univariate analysis was performed to present the mean number of fibroblast cells and the standard deviation. Furthermore, an analysis was carried out to assess the difference in the mean number of fibroblast cells between groups using the one-way ANOVA test. Results. The results of the one-way ANOVA test showed that there were significant differences between the test groups. Giving 10% and 15% turmeric extract was seen to be more potent in the initiation of the number of fibroblast cells than the treatment group that received povidone-iodine. Conclusion. Turmeric extract (Curcuma domestica Val.) concentration of 15% was the most effective concentration as an effort to increase the number of fibroblast cells after tooth extraction in Wistar rats.

The effectiveness of natural tooth whitening using herbal substances on coffee-induced tooth discoloration Purba, Member Reni; Tarigan, Gabriel C.J.; Lokanata, Shieny
Buletin Kedokteran & Kesehatan Prima Vol. 4 No. 1 (2025): March
Publisher : Fakultas Kedokteran, Kedokteran Gigi, dan Ilmu Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/bkkp.v4i1.6793

Tooth discoloration can be caused by many factors, including food and beverages. One of the drinks that can affect tooth color is coffee. Tooth discoloration can be overcome by teeth whitening procedures, which often use chemicals that can cause tooth sensitivity. Therefore, in this study, researchers are looking for safer alternatives, namely using herbal ingredients such as kepok banana peel and pineapple stem. This study used a laboratory experimental method with a pre-test and post-test only research design with control groups. The sample consisted of 27 central and lateral incisor teeth, which were divided into three treatment groups. The samples were then soaked for 14 days in coffee solution, and then group one was soaked in 100% kepok banana peel extract, group two was soaked in 100% pineapple stem extract, and group three as a control group was soaked in 6% hydrogen peroxide. All samples were soaked for two days in an incubator. The results of the one-way ANOVA test showed that there was a significant color difference (p<0.05) between the three treatment groups. The Post Hoc LSD test showed there was a significant difference between the 6% hydrogen peroxide group and the two extract groups, and also a significant difference between 100% kepok banana peel and 100% pineapple stem extract. Each group has an effect on tooth discoloration. Hydrogen peroxide is indeed the group with the highest tooth color change but has an effect that can make teeth sensitive. The banana peel and pineapple stem extract groups also had a significant and safer effect on teeth. 100% kepok banana peel extract and 100% pineapple stem extract have an effect on tooth discoloration but are not better than hydrogen peroxide. In addition, 100% pineapple stem extract has a better ability in teeth whitening than 100% kepok banana peel extract.

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