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All Journal Jurnal Kesehatan Medika Saintika JURNAL KESEHATAN PERINTIS
Suraini, Suraini
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Deteksi Gen Jamur Candida albicans pada Saliva Penderita Diabetes Melitus Dengan Metode Polymerase Chain Reaction Sophia, Anggun; Suraini, Suraini; Arhesta, Silvi
JURNAL KESEHATAN PERINTIS Vol. 11 No. 2 (2024): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v11i2.1101

Abstract

Diabetes mellitus is a predisposing factor that increases the risk of oral candidiasis, where the oral cavity of patients becomes an ideal environment for fungal growth due to excess glucose in saliva secretion. This glucose accumulation, along with disturbances in the salivary glands, can trigger the growth of Candida albicans, making it a significant health issue. To accurately detect the presence of Candida albicans, the Polymerase Chain Reaction (PCR) method is used, which allows for the identification of fungal genes with high sensitivity. The aim of this study is to detect the Candida albicans gene in the saliva of women with diabetes mellitus using the Polymerase Chain Reaction method. This research is an analytical study with a cross-sectional design. The samples used in this study are saliva from 10 diabetes mellitus patients experiencing oral pain symptoms. The examination procedures include saliva sample collection, fungal culture testing using Sabouraud Dextrose Agar (SDA), conventional identification, and fungal gene detection using the PCR method. This process includes fungal sample preparation, DNA extraction, DNA amplification, and agarose gel electrophoresis. The results show that 1 respondent tested positive for Candida albicans in the saliva sample, with a percentage of 10%, while 90% were negative. Positive test results were determined through macroscopic analysis of fungal cultures, microscopic examination with Gram staining, and further testing using the germ tube method. The positive sample was then analyzed for fungal gene detection using the PCR method with Internal Transcribed Spacer (ITS), where Candida albicans was detected in the ITS region with a product length of 600 bp.
Isolasi dan Identifikasi Molekuler Candida albicans dari Sekret Keputihan Menggunakan Metode PCR Sophia, Anggun; Suraini, Suraini; Susanto, Vetra; Niken, Niken
Jurnal Kesehatan Medika Saintika Vol 16, No 2 (2025): Desember 2025
Publisher : Stikes Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jkms.v16i2.30704

Abstract

Candida albicans is one of the major pathogenic fungi responsible for pathological vaginal discharge in women. This study aimed to isolate and identify C. albicans from vaginal discharge samples using conventional methods and molecular confirmation based on Polymerase Chain Reaction (PCR). A total of five vaginal discharge samples were collected and cultured on selective media. The isolation results showed that four samples (SC2–SC5) exhibited colony characteristics consistent with C. albicans, while one sample (SC1) demonstrated morphological features suggestive of the genus Aspergillus. Conventional identification through macroscopic and microscopic examination, as well as the germ tube test, confirmed that the four isolates were strong candidates for C. albicans. Molecular identification using specific primers targeting the Internal Transcribed Spacer (ITS) region indicated that only one isolate produced a distinct DNA band at approximately ±600 bp, which corresponds to the specific ITS fragment size for C. albicans. The SC3 isolate was confirmed as C. albicans based on molecular analysis. These findings highlight the importance of PCR-based confirmation to improve the accuracy of fungal identification, particularly in cases of pathological vaginal discharge.
Co-Authors Anggun Sophia Arhesta, Silvi Niken Niken Susanto, Vetra