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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Research Journal of Life Science Journal of Tropical Plant Protection Agro Bali: Agricultural Journal Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Jurnal HPT (Hama Penyakit Tumbuhan)
Sektiono, Antok Wahyu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology Social Sciences

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Potential of Wild Yeast from Banana to Control Colletotrichum musae Fungi Caused Anthracnose Disease and Its Short Antagonistic Mechanism Assay Muhibuddin, Anton; Sektiono, Antok Wahyu; Sholihah, Dewi Maratus
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.745 KB)

Abstract

Anthracnose caused by Colletotrichum musae is one of several important disease in banana fruit. An effective, cheap, and safe control method are necessary as a postharvest disease control alternative. This research aimed to obtain and identify wild yeast from banana which was isolated from its peel that has antagonist ability effectively in controlling anthracnose disease of banana fruit.  This research was started with an isolation of C. musae and yeast from banana, followed by in vivo assay with count percentage antagonist level, slide culture, and in vivo assay to know pathogen incubation stage and incident disease level. The yeast that isolated from ambon variety was Candida sp. and Pichia sp. Metchnikowia sp. from kepok variety respectively.
ANALISIS KELAYAKAN DETEKSI CEPAT PENYAKIT HAWAR DAUN TANAMAN KENTANG PADA FASE AKHIR MENGGUNAKAN UAV: LATE BLIGHT FEASIBILITY ANALYSIS IN POTATOES USING UAV FOR QUICK DETECTION IN LATE-STAGE Nita, Istika; Putra, Aditya Nugraha; Sektiono, Antok Wahyu; Riza, Sativandi; Wicaksono, Kurniawan Sigit; Sholikah, Dinna Hadi; Kristiawati, Wanda; Rahma, Melati Julia
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 11 No. 3 (2023)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2023.011.3.2

Abstract

Produksi kentang di Indonesia berkontribusi + 0,3% dari total produksi dunia sebesar + 388.191.000 ton. Kentang merupakan komoditas hortikultura esensial di Indonesia dengan permintaan sekitar 2,82 kg ha-1 kapita-1 pada tahun 2021. Saat ini terjadi defisit ketersediaan kentang yang mencapai 4.845.910 ton yang diperparah dengan terus menurunnya produksi kentang nasional (1.164.738 ton). Penyakit hawar daun (Phytophthora infestans) merupakan salah satu masalah utama penyebab penurunan produksi kentang (kehilangan hasil antara 10-100%). Penyebaran penyakit hawar daun sulit untuk diidentifikasi secara real time, sehingga diperlukan teknologi tepat guna yang dapat memberikan informasi secara cepat dan akurat. Penelitian ini bertujuan untuk melihat bagaimana foto udara (dari UAV) memperkirakan sebaran penyakit hawar daun pada kentang. Foto UAV diubah menjadi indeks NDVI, RDVI, SAVI, SR, ARVI-2, DVI, IPVI, dan GCI. Data pengukuran indeks penyakit hawar daun akan dikorelasikan dan dipilih yang terbaik untuk mendapatkan rumus regresi distribusi spasial penyakit hawar daun. Lokasi penelitian berada di Kecamatan Bumiaji, Kota Batu, Indonesia. Titik pengamatan di lapangan sebanyak 50 titik pengamatan untuk setiap luasan 3 Ha. Hasil penelitian menunjukkan bahwa semua indeks berkorelasi positif (> r tabel 0,34). Korelasi tertinggi pada estimasi model dari indeks NDVI (0,72). Kondisi ini sejalan dengan koefisien regresi (R2) pada NDVI yang mencapai 0,51 dengan persamaan y = 20,779 * (angka indeks NDVI) + 49,146. Analisis t-paired menunjukkan bahwa t hitung pada model (-1,10) ada pada grafik t-tabel (2,16), dan ini menegaskan bahwa rumus tersebut dapat diandalkan untuk digunakan.
UJI POTENSI JAMUR PENGHASIL IAA SEBAGAI PENGENDALI HAYATI PATOGEN LAYU Fusarium oxysporum PADA TANAMAN CABAI: INVESTIGATING THE POTENTIAL OF IAA-PRODUCING FUNGUS AS A BIOLOGICAL CONTROL OF FUSARIUM WILT ON CHILI Khoirunisa, Fenti Rahma; Sektiono, Antok Wahyu; Djauhari, Syamsuddin; Aini, Luqman Qurata
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2024.012.1.3

Abstract

Pemanfaatan mikroba antagonis sebagai agens pengendali hayati penyakit tumbuhan telah banyak dilakukan. Diketahui beberapa jamur antagonis mampu menghasilkan hormon Indole Acetic Acid (IAA) sehingga memiliki peranan yang lengkap dalam menunjang pertumbuhan tanaman karena dapat bertindak sebagai bioprotectant dan biofertilizer. Saat ini berbagai upaya untuk meningkatkan efektifitas pengendalian dengan agens hayati terus dilakukan guna mendapatkan cara pengendalian hayati yang efektif dan efisien sehingga memiliki daya tarik bagi pelaku budidaya pertanian. Penelitian ini bertujuan untuk mengetahui potensi jamur penghasil IAA dalam meningkatkan pertumbuhan tanaman serta menghambat patogen Fusarium oxysporum pada tanaman cabai. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 5 kali ulangan. Hasil penelitian ini didapatkan kedelapan isolat jamur memiliki daya hambat lebih dari 60% dan persentase daya hambat tertinggi pada hari ketujuh terdapat pada isolat jamur G sebesar 80,89%. Sedangkan hasil uji IAA secara kualitatif menggunakan reagen Salkowski dari kedelapan isolat jamur didapatkan dua isolat jamur yang mengalami perubahan warna menjadi merah muda yaitu isolat jamur AMR dan AR dengan konsentrasi masing-masing sebesar 22,29 ppm dan 11,65 ppm. Penelitian yang dilakukan di Rumah Kaca didapatkan hasil bahwa perlakuan P1 pada 7 MST efektif untuk meningkatkan tinggi tanaman dan perlakuan P2 pada 7 MST efektif menambah jumlah daun tanaman cabai rawit. Selain itu, semua perlakuan jamur antagonis berpotensi sebagai agens hayati dengan perlakuan P3 memiliki intensitas penyakit terendah pada 7 MST sebesar 10%.
Effect of Temperature on the Germination and Spread of the Mycelia of Sporisorium scitamineum Fungus Causes Smut Disease in Sugarcane Tasya, Tasya; Muhibuddin, Anton; Sektiono, Antok Wahyu; Hidayah, Nurul
Research Journal of Life Science Vol 11, No 1 (2024)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2024.011.01.3

Abstract

Sugarcane is presumed to be susceptible to smut disease caused by a basidiomycete fungus, Sporisorium scitamineum. One environmental condition that influences the disease's development is temperature. However, information related to smut disease, especially the influence of the environment (temperature) on the growth of S. scitamineum in sugarcane, is limited. Therefore, studying the effect of different temperature ranges on S. scitamineum growth is necessary. The research, which was arranged using a completely randomized design with 3 (replicates) consisted of two treatments, including temperatures (20, 25, 30, and 35°C) and incubation time (1, 2, 4, and 6 hours). The teliospore suspension was inoculated on a water agar medium and incubated at different times and temperatures. After each allocated time, the plates were taken from the incubator, and then germination of teliospores was observed under a compound microscope. In addition, we also conducted research on the effect of inoculation temperature (25, 30, and 35°C) on the mycelial distribution within sugarcane tissue. The bud setts were immersed in teliospore suspension for 10 minutes at different temperatures, then planted in the polybags and kept for one and two weeks. The results showed that temperature affected the germination of S. scitamineum teliospores, in which the highest germination (92,3%) occurred at 30°C. On the other hand, the lowest germination was at 20°C with 0% germination after 1 hour incubation time. The in vivo study revealed that the distribution of mycelia within sugarcane tissue was similar, either incubated in one or two weeks.
Perbanyakan agens hayati, pembuatan kompos, dan budidaya ikan guna menunjang implementasi sistem pertanian terpadu Sektiono, Antok Wahyu; Widyawati, Yuni; Yanuar, Adi Tiya; Swara, Suluh Elman; Mustofa, Oki
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 4 No. 3 (2023)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v4i3.20947

Abstract

Desa Simo memiliki luas wilayah 268,85 ha dengan 23% wilayahnya berupa lahan pertanian dan sebagian wilayah berupa bentang alam lainnya yang menyimpan potensi besar yang belum termanfaatkan secara optimal terutama guna pembangunan pertanian. Oleh karena itu, dilakukan kegiatan pemberdayaan masyarakat menggunakan metode Asset Based Community Development (ABCD). Praktik dalam kegiatan terfokus pada, 1) pengenalan dan perbanyakan agens hayati, 2) pembuatan kompos dari limbah ternak, 3) pembudidayaan ikan yang baik. Dengan adanya kegiatan tersebut diketahui pemahaman petani di desa Simo mengenai pemanfaatan beberapa potensi desa yang dapat dioptimalkan dalam pertanian mengalami perubahan. Masyarakat petani setempat mulai mengenal dan bahkan memperbanyak agens hayati sebagai pengendali hama dan penyakit tumbuhan, memanfaatkan kotoran ternak sebagai bahan baku kompos, serta mengelola kolam sebagai pertumbuhan dan perkembangan ikan.
Soil Fungus and Bacteria Antagonism Test to Control Phytophthora nicotianae in The In Vitro Istifada, Valia; Syib’li, Muhammad Akhid; Sektiono, Antok Wahyu; Ratdiana, Ratdiana; Limin, Atfritedy
Journal of Tropical Plant Protection Vol. 3 No. 2 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.2.5

Abstract

This study aims to determine the potential of antagonistic fungi and bacteria as controlling agents for Phytophthora nicotianae. The research was conducted at the Laboratory of Biological Control 1, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang. The method used is soil sampling, isolation, purification, identification and in vitro antagonism test. The antagonism test is done by the dual culture method. This study used a completely randomized design for eight treatments, including the control and each treatment was repeated four times. In this study, the antagonism test results were obtained between antagonistic fungi and Phytophthora nicotianae, which Penicillium sp. (PEG01) had the highest percentage of inhibition, and the one with the lowest total diameter was Trichoderma sp. (TRC01). Moreover, the antagonism test results were obtained between antagonistic bacteria and Phytophthora nicotianae, which bacteria C had the highest percentage of inhibition and the lowest total diameter.
Efficacy of Biofungicide with Active Ingredients Trichoderma sp. Against Late Blight Disease (Phytophthora infestans) in Potato Plants Abadi, Abdul Latief; Trianti, Irisa; Choliq, Fery Abdul; Sektiono, Antok Wahyu; Yulianti, Novi Dwi
Agro Bali : Agricultural Journal Vol 7, No 1 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i1.1591

Abstract

The cultivation of potatoes in Indonesia faces challenges, particularly a decline in production attributed to the devastating impact of late blight caused by the fungus P. infestans. Traditional control methods involve the frequent use of chemical pesticides, which pose environmental and health risks. To address this, the research aimed to assess the efficacy of biofungicide containing Trichoderma sp. as a biological agent in controlling late blight on potatoes. The study, conducted in Sumberbrantas Village, Bumiaji District, Batu City, East Java, and the plant disease laboratory at Brawijaya University from January to April 2023, employed both in vivo and in vitro experiments. A Randomized Block Design (RBD) was used for in vivo research, while a Completely Randomized Design (CRD) was employed for in vitro studies, each comprising five concentration treatments with five repetitions. Parameters such as disease intensity, efficacy level, potato production, and biofungicide inhibition were observed. Analysis of variance and the Tukey Honestly Significant Difference (HSD) test were applied to the data and processed using Microsoft Excel. The results indicated that biofungicide with Trichoderma sp. concentrations of 6 ml/l, 4.5 ml/l, 3 ml/l, and 1.5 ml/l effectively reduced the intensity of late blight on potatoes, with efficacy levels exceeding 50%. The highest potato production occurred with a concentration of 6 ml/l, yielding 15.44 tons/ha, followed by 14.67 tons/ha for the 4.5 ml/l concentration. The biofungicide with a concentration of 6 ml/l exhibited the lowest disease intensity at 14.28% and the highest efficacy at 83.08%. Inhibition tests demonstrated that concentrations of 6 ml/l and 4.5 ml were highly effective, inhibiting P. infestans growth by an average of 62.04% and 59.90%, respectively. These findings highlight the potential of biofungicide with Trichoderma sp. in managing late blight on potatoes, providing a sustainable and environmentally friendly alternative to chemical pesticides.
Co-Authors Abdul Latief Abadi Aditya Nugraha Putra, Aditya Nugraha Anton Muhibuddin Fery Abdul Choliq Irisa Trianti Istifada, Valia Istika Nita Khoirunisa, Fenti Rahma Kristiawati, Wanda Kurniawan Sigit Wicaksono, Kurniawan Sigit Limin, Atfritedy Luqman Qurata Aini Melati Julia Rahma Mustofa, Oki NURUL HIDAYAH Ratdiana, Ratdiana Riza, Sativandi Sholihah, Dewi Maratus Suluh Elman Swara Syamsuddin Djauhari Syib’li, Muhammad Akhid Tasya, Tasya Widyawati, Yuni Yanuar, Adi Tiya Yulianti, Novi Dwi