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All Journal Jurnal Penelitian Saintek Jurnal Ilmu Dasar Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi BIOEDUKASI Sciscitatio : Journal for Biological Science Berkala Penelitian Hayati Jurnal Abdi Masyarakat Indonesia COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Journal of Health and Nutrition Research Journal of Comprehensive Science SERVIRISMA: Jurnal Pengabdian kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Bioma : Jurnal Ilmiah Biologi
CHARIS AMARANTINI
Biology Department, Universitas Kristen Duta Wacana, Jalan Dr Wahidin Sudirohusodo 5-19, Yogyakarta 55224, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Identifikasi Cemaran Bakteri Staphylococcus sp. dan Jamur Aspergillus sp. pada Produk Roti untuk Meningkatkan Keamanan Pangan Wattimury, Angelia; Samodra, Eunike Andriani Marganingrum; Ferdian, Evelyn; Budiarso, Tri Yahya; Amarantini, Charis
Journal of Comprehensive Science Vol. 3 No. 8 (2024): Journal of Comprehensive Science (JCS)
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jcs.v3i8.838

Abstract

Roti merupakan salah satu produk makanan cepat saji karena sangat mudah didapatkan dan sebagai salah satu bahan makanan pokok yang dikonsumsi secara global. Namun, roti mudah mengalami kerusakan yang dapat disebabkan oleh bakteri dan jamur yang mempengaruhi kesehatan. Penelitian ini bertujuan unutk mengidentifikasi cemaran atau kontaminan bakteri dan jamur pada produk roti. Sampel roti diperoleh dari beberapa kantin sekolah, warung, dan pasar yang berada di Kota Yogyakarta. Dalam mengidentifikasi bakteri dibutuhkan medium Nutrient Agar, Mannitol Salt Agar, dan Baird Parker Agar, sedangkan jamur ditumbuhkan pada medium Malts Extract Agar pada cawan petri. Seleksi koloni bakteri menjadi koloni tunggal dilakukan dengan uji biokimia. Uji konfirmasi bakteri dilakukan dengan API Staph dan uji molukuler dengan gen penanda nuc dan sea menggunakan PCR. Isolat jamur diidentifikasi secara makromorfologi melalui pengamatan bentuk koloni dan mikromorfologi melalui pengamatan sel dengan teknik slide culture. Jenis bakteri yang ditemukan pada sampel yaitu Staphylococcus aureus (teridentifikasi API 52,8%, 97,7%, dan 97,7%). Isolat terduga S. aureus dengan persentase ID 52,8% terdeteksi memiliki gen sea tetapi tidak memiliki gen nuc. Isolat terduga. Jenis jamur yang ditemukan pada sampel yaitu Aspergillus niger.
Preliminary Selection of Potential Probiotic Lactic Acid Bacteria Isolated from Cocoa Beans (Theobroma cacao) Amarantini, Charis; Prakasita, Vinsa Cantya; Sema, Maria Trivonia
Biosfer: Jurnal Tadris Biologi Vol 12 No 1 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i1.7128

Abstract

This study aims to isolate and identify LABs that spontaneously grow fermented cocoa beans that have the potential as probiotics. The isolation was carried out selectively using an MRS agar medium supplemented with 1% CaCO3. LAB's potential as a probiotic was selected based on the acid resistance test, bile salt resistance test, and antimicrobial activity test. The results showed that three isolates of lactic acid bacteria, namely strains K1B1, K2T2, and K3B4 were tolerant of gastric acid's pH conditions (pH 2.5, pH 3, and pH 4). They also can survive the bile salts (0.3%, 0, 5%, and 1%) and have antibacterial characteristics. The three isolates were identified as Lactobacillus Plantarum species (ID 99.9%).
The Microbiological Hazards of Enterobacter spp. Contamination in Street Food in Developing Countries: A Literature Review Pareakan, Jefyne Mali’; Amarantini, Charis; Budiarso, Tri Yahya
Journal of Health and Nutrition Research Vol. 4 No. 2 (2025)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v4i2.424

Abstract

Street food is widely consumed by people in developing countries and often poses health risks due to bacterial contamination. The consumption of contaminated food has been linked to more than 200 types of diseases, resulting in 600 million cases and 420,000 deaths each year. One commonly found bacterium is Enterobacter, which can be pathogenic and exhibit resistance to various antibiotics. This review aims to assess the dangers of Enterobacter contamination in street food, including the level and source of contamination, pathogenicity, and management strategies. The review followed PRISMA 2020 guidelines, with literature searches performed in scientific databases such as PubMed and Google Scholar using Boolean operators and relevant keywords. Articles were selected based on specific inclusion criteria, analyzed, and grouped for ease of interpretation. The results showed that Enterobacter contamination is prevalent in developing countries such as Nigeria, Ghana, Ethiopia, Pakistan, India, and Indonesia. Nigeria recorded the highest prevalence, with E. aerogenes reaching 100% in ready-to-eat rice. The primary source of contamination is raw materials, particularly wheat flour, which contains E. sakazakii with a prevalence of 100%. Enterobacter can cause various serious infections, including urinary tract infections, meningitis, pneumonia, and septicemia. Efforts to reduce Enterobacter contamination in street food should include the implementation of hygiene and sanitation practices, the use of safe raw materials, proper processing and storage methods, education for vendors, and routine food safety supervision. These findings are crucial for supporting public health interventions and developing effective food safety policies.
COLIFORM CONTAMINATION LEVEL OF WASHING WATER AT STREET VENDORS TENT STALLS IN YOGYAKARTA Amarantini, Charis; Budiarso, Tri Yahya; -, Tri Wahyuni Sekar Sari
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.365

Abstract

Food quality in Indonesia is significantly influenced by street vendors, particularly from a microbiological aspect. The use of unclean washing water in these settings can cause tableware contamination, increasing the risk of food-borne diseases. Therefore, this research aimed to determine the level of coliform contamination in washing water of street vendors. A total of 21 samples were collected from different locations, isolated, and identified morphologically and biochemically. These samples were resuscitated in 1% peptone medium for 12 hours and inoculated on Chromocult Coliform Agar (CCA) medium. Purified coliform colonies were tested morphologically and biochemically to the genus level and confirmed using API 20E KIT reagents. The results showed coliform contamination levels ranging from 5.1 x 105 to 2.7 x 108 CFU/mL, exceeding the quality standard. Confirmation results from testing using API 20E with ID ≥ 95% found bacteria contaminants, including Escherichia coli, Klebsiella pneumoniae subsp. Pneumoniae and Pantoea spp. These results showed the urgent need to recognize the risks associated with tableware contamination at tent stalls. Keywords: API 20E, Coliform, Street Vendors, Washing Water.
Identifikasi Bacillus sp. Pada Makanan Ringan berbasis Umbi-umbian Melalui Analisis Molekuler Gen Toxin Hbl dan Nhe Andriani, Eunike Marganingrum; Wattimury, Angelia; Budiarso, Tri Yahya; Amarantini, Charis
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 5 (2024): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v4i5.1294

Abstract

Foodborne disease atau penyakit yang disebabkan karena konsumsi makanan dapat disebabkan oleh berbagai mikroorganisme. Salah satu mikroorganismenya adalah Bacillus cereus sebagai bakteri gram positif dan fakultatif anaerob yang dapat mencemari dari lingkungan mampu melakukan pemulihan dari makanan dan bahan pangan yang lain. Hal yang membuat Bacillus sp. Dapat bertahan di kondisi ekstrem adalah keberadaan spora terutama saat berada di bahan pangan yang sudah dikeringkan atau dibuat dalam bentuk bubuk. Kasus kontaminasi kelompok Bacillus cereus yang mendatangkan foodborne illness ditemukan dalam produk kentang. Bacillus cereus dan terkonsumsi dapat memberikan dampak terhadap sakit pencernaan berupa sakit perut dan diare tidak berdarah yang terjadi 4 - 16 jam setelah konsumsi. Gejala-gejala tersebut disebabkan oleh produksi diarrheal toxin hemolysin BL, Hbl, enterotoksin nonhemolitik Nhe dan sitotoksin CytK pada usus manusia. Penelitian ini bertujuan untuk melakukan identifikasi Bacillus sp. Pada produk makanan ringan jenis umbi-umbian berdasarkan keberadaan gen pengkode toxin Hbl dan Nhe. Metode yang dilakukan dengan isolasi awal menggunakan media MYP Agar, uji MR, pengecatan gram, endospora, dan analisis hasil PCR gen toxin Hbl dan Nhe. Hasil pada penelitian ini menunjukkan sampel keripik kentang kemasan tercemar Bacillus cereus berdasarkan amplifikasi gen nheA, nheB, nheC pengkode toksin Nhe dan gen hblA, hblD, hblC pengkode gen toksin Hbl sedangkan terduga Bacillus subtilis tidak terdapat gen nheB.
The Effect of Corn (Zea mays) Cob Extract on the Growth of Bifidobacteria Sejati, Ribka Ananda; Ariestanti, Catarina Aprilia; Amarantini, Charis
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 3 (2023): October 2023
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v8i3.6942

Abstract

Corncob is a source of dietary fiber, contains xylan polysaccharides (12.4%–31.94%), and is classified as the highest xylan-producing source compared to other agricultural wastes. Human digestive enzymes cannot degrade xylan but can be enzymatically degraded by lactic acid bacteria (LAB) through fermentation. This research aimed to evaluate the effect of corncob extract on the growth of Bifidobacterium. Corncob extract is obtained through alkaline extraction. The growth of Bifidobacterium bifidum FNCC 0462 and Bifidobacterium longum FNCC 0463 was observed through in vitro fermentation for 48 h using the total plate count (TPC) method. Bifidobacterium bifidum FNCC 0462 showed the ability to ferment corn cob extract for 48 h of fermentation with the highest growth at 8 h of fermentation (4,08 log10 CFU/mL) while B. longum FNCC 0463 was able to grow up to 16 h of fermentation only. The results indicated that corncob extract could support B. bifidum FNCC 0462 growth.  
Co-Authors -, Tri Wahyuni Sekar Sari Andriani, Eunike Marganingrum Ariestanti, Catarina Aprilia Ernawati, Lussy Ferdian, Evelyn Guruh Prihatmo Haripurnomo Kushadiwijaya Ilham, Jessika Langkah Sembiring Lifiana, Aulia Maria Trivonia Sema Pare, Regina Londong Pareakan, Jefyne Mali’ Raden Gunawan Santosa Rangga, Febby Alvanda RATNA SETYANINGSIH Samodra, Eunike Andriani Marganingrum Sejati, Ribka Ananda Sema, Maria Trivonia Suryanto, Regina TRI YAHYA BUDIARSO Vico, Yoel Vinsa Cantya Prakasita Wattimury, Angelia Widya Asmara Yetli Oslan