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Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor Palensina, Gea Ocktiah; Rosita, Leni; Sagala, Zuraida
Bioinformatics and Biomedical Research Journal Vol. 4 No. 1 (2021): Vol 4 No 1 (2021)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/bbrj.04.01.02

Abstract

Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.
PENGARUH HIDROTERAPI TERHADAP PENINGKATAN SIRKULASI DARAH PERIFER PADA PASIEN DM DI WILAYAH PUSKESMAS KELAPA DUA, TANGERANG YUNINGSIH, YUNINGSIH; ROSITA, LENI
Edu Dharma Journal :Jurnal penelitian dan pengabdian masyarakat Vol 6, No 2 (2022): Edu Dharma Journal: Jurnal Penelitian Dan Pengabdian Masyarakat
Publisher : Sekolah Tinggi Ilmu Kesehatan Widya Dharma Husada Tangerang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52031/edj.v6i2.430

Abstract

ABSTRACTIndonesia is one of the countries with a high level of DM alertness. People with Diabetes Mellitus will reach 6.2 percent or 10 million in 2022. Problems that often occur among diabetes mellitus patients are due to high glucose levels in the blood, causing damage to blood vessels, nervous tissue, and other organ structures. One of the complications of diabetes mellitus occurs a lot: foot injuries and foot shape differences. The disruption of blood flow affects the appearance of Stenosis and the number of thrombosis. This study aims to determine whether hydrotherapy's impact on increasing peripheral blood circulation is seen from the Ankle Brachial Index (ABI) in the Kelapa Dua area DM patients. This study is a descriptive quantitative study using paired sample t- test. There were 80 patients, and 20 patients were taken as samples. The results showed that hydrotherapy did not affect changes in the Ankle Brachial Index in DM patients, where p = 0.258 0.05. So the null hypothesis is accepted where this study concludes that hydrotherapy has no effect on the measurement of ABI in DM patients in the Kelapa Dua Health Center area of Tangerang.ABSTRAK Indonesia  termasuk Negara yang memiliki tingkat kewaspadaan DM Cukup tinggi. Pengidap Diabetes Melitus mencapai 6,2 persen atau 10 juta pada 2022. Permasalahan yang banyak terjadi di kalangan pasien diabetes melitus karena  tingginya kadar glukosa dalam darah, sehingga menyebabkan  rusaknya pembuluh darah dan jaringan saraf dan struktur organ lainnya.Salah satu komplikasi  diabetes melitus banyak terjadi luka kaki dan perbedaan bentuk kaki. Adapun gangguan aliran darah  berdampak muncul Stenosis dan  banyaknya thrombosis. Penelitian ini bertujuan untuk mengetahui apakah ada dampak hidroterapi  terhadap peningkatan sirkulasi darah perifer dilihat dari Angka Brachial Index (ABI) pada pasien DM di wilayah Kelapa Dua. Penelitian ini merupakan   penelitian kuantitatif deskriptif, dengan menggunakan uji beda paired sample t test.  Terdapat sejumlah 80 pasien, dan 20 pasien diambil sebagai sampel. Hasil penelitian menunjukkan  bahwa hidroterapi  tidak memiliki pengaruh terhadap perubahan Ankle Brachial Index pada penderita DM dimana nilai p = 0,258 0,05. Maka Hipotesis null diterima dimana penelitian ini kemudian menyimpulkan bahwa  tidak ada pengaruh Hidroterapi  terhadap pengukuran ABI pada pasien DM di wilayah Puskesmas Kelapa Dua Tangerang. 
Implementation of Hypovolemia Management with Hypovolemia Nursing Problems in Open Fracture Patients Rosita, Leni; Hibahtulloh, Arif
AACENDIKIA: Journal of Nursing Vol. 3 No. 2 (2024): AACENDIKIA: Journal of Nursing
Publisher : Althar Cendikia Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59183/aacendikiajon.v3i2.45

Abstract

Fracture is a disruption of bone continuity that causes tissue and blood vessel damage, resulting in bleeding around the fracture and soft tissue, which can cause hypovolemia. The most effective way to overcome this problem is with hypovolemia management. The purpose of this study was to determine the application of hypovolemia management in open fracture patients who experience hypovolemia. The method used in this compilation is a descriptive type of compilation. Qualitative compilation is a compilation of research that is descriptive in nature and tends to use analysis with the study subjects in this case being 2 patients who experienced open fracture health problems with hypovolemia nursing problems with patient criteria: Patients with open fractures, experiencing hypovolemia nursing problems, and willing to undergo hypovolemia management. Data collection according to the assessment format, focused on subjective and objective data to facilitate data collection regarding the application of hypovolemia management in fracture patients. The diagnosis made was hypovolemia related to active fluid loss, with hypovolemia management intervention. The conclusion of the case study showed that the condition of the two patients began to improve after hypovolemia management was carried out.
Penyuluhan Kesehatan Keluarga tentang Penerapan Latihan Nafas Dalam dan Batuk Efektif pada Klien dengan ISPA (Infeksi Saluran Pernafasan Akut) Rosita, Leni; Aprilia, Putri
Mega Buana Journal of Innovation and Community Service Vol. 3 No. 2 (2024): Desember Tahun 2024
Publisher : LPPM Universitas Mega Buana Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59183/vwvjfw72

Abstract

ISPA merupakan salah satu penyakit berbasis lingkungan yang menyebar melalui udara. Penyakit ini dapat menular apabila virus atau bakteri yang terbawa droplet terhirup oleh orang sehat. Peran keluarga dalam merawat anggota keluarga dengan ISPA sangat penting terutama dalam mencegah kegawatan pada ISPA seperti memberi obat, memperhatikan kebersihan rumah atau lingkunga rumah.Tujuan dari penyuluhan ini adalah adanya peningkatan pengetahuan anggota keluarga mendemonstrasikan latihan nafas dalam dan batuk efektif. Metode dalam penyuluhan kesehatan ini meliputi pre-test pada awal kegiatan untuk mengetahu pengetahuan awal peserta sebelum diberikan edukasi kesehatan, kemudian dilakukan edukasi kesehatan berupa penyuluhan tentang pengertian, tujuan, kondisi penyakit yang boleh dan tidak boleh dilakukan latihan nafas dalam dan batuk efektif. Setelah peyuluhan selesai dilanjutkan dengan post-test untuk mengukur pengetahuan peserta setelah diberikan intervensi. Hasil kegiatan diperoleh peningkatan pengetahuan peserta yang sebelumnya memiliki tingkat pengetahuan baik dengan rata-rata nilai pre-test 20 (dari 6 soal hanya 1 yang dijawab dengan benar) setelah dilakukan penyuluhan kesehatan tingkat pengetahuan peserta menjadi baik dengan nilai post-test 100 (keluarga dapat menjawab benar semua). Kesimpulan: Semua peserta dalam kegiatan pengabdian masyarakat dapat memahami tentang penerapan latihan nafas dalam dan batuk efektif. Saran dari kegiatan pengabdian ini adalah diharapkan selalu ada upaya bersama untuk memberikan edukasi kesehatan kepada masyarakat, terkhusus penatalaksanaan ISPA