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Journal : JBIO: jurnal biosains (the journal of biosciences)

ISOLATION AND CHARACTERISATION OF Pb RESISTANT BACTERIA FROM MARINE SEDIMENT AFFECTED BY INCONVENTIONAL TIN MINING AT SAMPUR BEACH, BANGKA TENGAH DISTRICT Ina Miranti; Rahmad Lingga; Verry Andre Fabiani
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 2 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i3.24600

Abstract

Offshore tin mining can causes heavy metal contamination, one of which is lead (Pb) metal. Lead pollution can causes environmental damage and  toxic  to living organisms. One of the methods to reduce lead pollution was by bioremediation techniques. Resistant bacteria were potential to be used as bioremediation agent. This research aimed to determine the lead level in Sampur coastal waters and to obtain bacteria that have resistance to lead (Pb). The research method was purposive sampling to take a sample of water and sediment. The AAS test results indicated that the Pb level in seawater is higher than sediment. A normal sea water pH can caused the Pb solubility in the water to be stable. The Pb level in seawater was passed the established quality standards, so that potentially cause toxicity for the aquatic biota. The highest Pb levels in sediment was found in vicinity of tin mining activities area. The isolation results showed that the number of bacteria ranged from 11,105 – 34,105 CFU/g. The higher Pb levels in sediment causing the number of bacteria will be lower. There were 7 isolates that resistant of Pb 100 ppm and constitute bacteria from the genus Alcaligens, Meniscus, Neisseria, Erythrobacter, and Alteromonas.
ANTIBACTERIAL ACTIVITIES OF RUKAM LEAVES (Flacourtia rukam Zoll. & Moritzi) AGAINST Staphylococcus aureus AND Escherichia coli Henri Henri; Rahmad Lingga
JBIO: jurnal biosains (the journal of biosciences) Vol 7, No 2 (2021): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v7i3.21595

Abstract

Traditional herbal medicines are now attracted significant attention used as the basis for modern medicines, including the plant of rukam (Flacourtia rukam) from the Flacourtiaceae family which is known by the public as medicine. This research aimed to explore the potential antibacterial activity for the plant of rukam (F.rukam) against bacterial Staphylococcus aureus and Escherichia coli. This research method was to first test the secondary metabolite content of F. rukam by using four solvents. The antibacterial activity extract from leaves of F. rukam was examined against S. aureus and E. coli. The antibacterial activity was assessed in the concentration include 20%, 40%, 60%, 80%, and 100% by disc diffusion method. Phytochemical test results such as phenolics, flavonoids, saponins, steroids, and alkaloids are secondary metabolites of the F. rukam plant. The highest zone of inhibition is at a concentration of 60% using methanol solvent, which is an average of 8.95 ± 1.84 in S. aures isolates, while the E. coli bacterial isolates have an average of 9.03 ± 0.95 . This result was different from using ethanol solvent where the highest inhibition zone was at a concentration of 20%, respectively 7.73 ± 2.79 in S. aureus isolates and 6.61 ± 2.18 in E. coli bacteria isolates. Antibacterial efficacy shown by this plant F. rukam provides a scientific basis and thus validates traditional use.
IDENTIFICATION AND PHYTOCHEMICAL TESTS OF FUNGI MUSHROOMS AND HONEY SPECIALLY BANGKA BELITUNG Eva Dewi Rosmawati Purba; Rachmawati Felani Djuria; Rahmad Lingga
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.31428

Abstract

Pelawan Bitter Honey and Pelawan Mushroom are typical Bangka biodiversity found in Central Bangka Regency. The Pelawan mushroom grows in Bangka Belitung Islands Province and generally used as a food ingredient and has the potential to be developed as a source of natural immunomodulator agent. Phenolic compounds are known to have immunomodulatory activities that can improve the immune system. In addition, based on preliminary study, honey was also used by local people to strengthen the immune system. The purpose of this study was to identify the content of active compounds from Pelawan honey and Pelawan mushrooms. This research was an experimental study with several stages of testing including phytochemical screening, Thin Layer Chromatography and Extract Standardization tests. The results showed that based on phytochemical test of Pelawan honey contained flavonoids and alkaloids, while based on the Thin Layer Chromatography (TLC) test contained flavonoids and alkaloids. Phytochemical test of Pelawan mushroom showed the existence of Phenolic and Alkaloids, while based on TLC test containing flavonoids with an average Rf value of 0.56.  The standardization of Pelawan mushroom extract produced a total ash content of 28%, drying shrinkage of 40.88 g /mL, specific gravity 0.67 g/mL and water soluble extract content of 59.01% and ethanol soluble extract content of 41.12%. From these results, it can be assumed that the pelawan honey and pelawan mushroom have the potential to be developed as immunomodulatory agent.