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Antasiswa Windraningtyas Rosetyadewi
Departemen Farmakologi Fakultas Kedokteran Hewan Universitas Gadjah Mada
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Efektivitas Fluoroquinolon Terhadap Isolat Bakteri Saluran Pencernaan Ular Sanca Batik (Python reticulatus) Agustina Dwi Wijayanti; Antasiswa Windraningtyas Rosetyadewi; Tri Untari
Acta VETERINARIA Indonesiana Vol. 1 No. 1 (2013): Januari 2013
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.686 KB) | DOI: 10.29244/avi.1.1.27-31

Abstract

Telah dilakukan penelitian tentang efektivitas antibiotika golongan fluoroquinolon (flumequin dan enrofloksasin) terhadap Salmonella dan E. coli yang diisolasi dari saluran pencernaan ular sanca batik (Python reticulatus). Penelitian ini bertujuan untuk mengetahui efektivitas fluoroquinolon terhadap infeksi saluran pencernaan pada ular dan reptil pada umumnya. Penelitian dilakukan dengan menggunakan 8 ekor sanca batik dewasa yang menderita gangguan pencernaan dengan lesi klinis berupa mouthrot. Sampel ulas kloaka dan mulut serta sampel darah diambil dari semua ular, untuk selanjutnya dilakukan uji mikrobiologis berupa isolasi dan identifikasi bakteri melalui media Brilliant Green Agar (BGA), Mc Conkay Agar (MCA), Triple Sugar Iron (TSI) dan media biakan murni. Isolat murni yang didapatkan adalah Salmonella spp. dan E. coli dan selanjutnya dilakukan uji sensitivitas bakteri terhadap flumequin dan enrofloksasin serta penentuan Minimum Inhibitory Concentration (MIC) untuk enrofloksasin. Hasilnya adalah kedua antibiotika efektif terhadap Salmonella dan intermediet terhadap E. coli. Nilai MIC enrofloksasin terhadap Salmonella adalah 2,5 μg/ml.
Profil Farmakokinetik Amikasin Pemberian Intravena Melalui Vena Sublingualis dan Coccygea pada Ular Sanca Batik (PHARMACOKINETIC PROFILE OF AMIKACIN ADMINISTERED INTRA VENOUSLY VIA SUBLINGUAL AND COCCYGEA VEINS IN BROGHAMMERUS RETICULATUS) Agustina Dwi Wijayanti; Slamet Rahardjo; Antasiswa Windraningtyas Rosetyadewi; Adi Tri Septyanto
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.492 KB)

Abstract

The research was conducted to find out the pharmacokinetic profile of amikacin in sanca batik snake(Broghammerus reticulatus) which is expected to be beneficial in the therapy management of reptiliaes.The eight of adult snakes with averages body weight of 2-14 kg were used and they were devided into twogroups (n=4). Amikacin (5 mg/kg bw) was given by sublingualis (anterior) or coccygea (posterior) venous ingroups, respectively. Blood samples were collected by intracardiac puncture to all snakes at minutes 1,5,10, 30, 60, 120, 240, 480, 960, 1440 (24 hours) and 2880 (48 hours) post administrations. Bloods werecollected using heparinized tubes and sentrifuged at 2500 G to obtain the plasma. The Plasma sampleswere stored at -200c. Plasma were firstly extracted with trichloroacetid acid solution 10% and then injectedinto High Performace Liquid Chromatography Shimadzu 6.1. The results of amikacin levels werestatistically lower on sublingualis vein administration as compared to that of coccygea vein administrationusing Student T-Test (P<0.05). The pharmacokinetic parameters were calculated with non compartementalmethod resulted for anterior application : Vd 3.6 L, clearance 0.066 mL/minute/kg, AUC 75.384 ug/mL.minute and for posterior application : Vd 0.78 L, T1/2 213.09 hours, clearance 0.427 mL/minute/kg,AUC 117.143,7 ug/mL/minute.
Isolasi dan Identifikasi Bakteri dari Tinja Orangutan Penderita Gangguan Gastrointestinal (BACTERIAL ISOLATION AND IDENTIFICATION IN FAECES OF ORANGUTAN WITH GASTROINTESTINAL DISTURBANCE) Michael Haryadi Wibowo; Antasiswa Windraningtyas Rosetyadewi; Agustina Dwi Wijayanti; Claudia Mona Airin
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.448 KB)

Abstract

Orangutans are among protected animals by the law. One of orangutans’ main health problems isgastrointestinal disease due to bacterial infection. Microbiological data of causative agent of illness inorangutan still not much reported scientifically. This research aim was to identify causative agent ofbacterial infection on gastrointestinal disorder in orangutan isolated from stool samples. The sampleswere collected from Yayasan Konservasi Alam Yogyakarta and Borneo Orangutan Survival, Semboja,Kalimantan Timur. Fresh fecal samples were collected using sterile swab and put them into a steriletransport media. To achieve pure cultures, bacterial isolation was performed by using plate streaking onselective media. Gram stain was done to confirm the cell uniformity and morphology. Bacterialidentification was performed according to Bergey’s Manual Determinative Bacteriology on some biochemicalcharacters to determine the isolated bacteria. The result showed that three bacteria were identified fromstool samples orangutan from Yayasan Konservasi Alam Yogyakarta, i.e.: Citrobacter amalonaticus,Providensia rustigianii, and Proteus mirabilis. Meanwhile, three bacteria, which were Klebsiella planticola,Enterobanter agglomerans and Escherichia coli, were also identified in samples taken from Borneo orangutan.
Pengimbuhan Fitobiotik dan Probiotik Meningkatkan Rasio Konversi Pakan dan Menurunkan Persentase Lemak Abdomen Ayam Pedaging Agustina Dwi Wijayanti; Antasiswa Windraningtyas Rosetyadewi; Ida Fitriana; Anggi Muhtar Pratama
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.987 KB) | DOI: 10.19087/jveteriner.2021.22.3.303

Abstract

Phytobiotic is a derivative compound from plants that have the benefits as medicine or growth promoter. The compound had studied on it’s beneficial effect as growth promoter in poultry. This study has deducted to know the effect of Macleaya cordata extract and Lactobacillus acidophilus, Lactobacillus debrueckii susp. bulgaricus, Lactobacillus rhamnosus, Enterococcus faecium, dan Streptococcus salivarius susp. Thermophilus as feed additive products toward the feed convertion rate (FCR) and the percentage of abdominal lipid. The 60 of day old chick were maintained and devided into four groups consist of 15 chicken each (n=5, r=3). Group A treated with phytobiotic (Macleaya extract) 0.125 mg/kg of feed, group B treated with probiotic Lactobacillus acidophilus, Lactobacillus debrueckii susp. bulgaricus, Lactobacillus rhamnosus, Enterococcus faecium, dan Streptococcus salivarius susp. Thermophilus (probiotic product) 0 ,100 mg/kg of feed, group C treated with both phytobiotic and probiotic dose of 0,075 mg/kg dan 0,050 mg/kg of feed, respectively, and group K which no additives in feed. Body weight measurement did in every week. At day- 30 the broilers were euthanized and abdominal lipid weighted. The body weight and abdominal lipid measurements were analizyd with one way ANOVA, FCR values evaluated descriptively. The results showed that FCR of group A,B,C were better than control group (K) (A=1.21;B=1.20;C=1.26;K=1.32). The body weight between groups had no significant difference (P> 0.05), meanwhile the weight of abdominal lipid of group A,B,C, were lower (P<0.05) than control group (K). The percentage of abdominal lipid compare to body weight of group B and C were lower than K. It concluded that supplementation of Macleaya cordata extract and Lactobacillus acidophilus, Lactobacillus debrueckii susp. bulgaricus, Lactobacillus rhamnosus, Enterococcus faecium, dan Streptococcus salivarius susp. Thermophilus had benefits to reduce the abdominal lipid in broilers and gave the better FCR values.
POTENSI ANESTETIKA KET-A-XYL® PADA KUCING JANTAN DOMESTIK DI YOGYAKARTA INDONESIA Maria Angelica Maryatmo; Antasiswa Windraningtyas Rosetyadewi; Aria Ika Septana; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69624

Abstract

Ket-A-Xyl® (Ket-A-Xyl® 20 ml, AgroVet, Peru) merupakan sediaan anastetika jadi yang telah banyak dipasarkan di Indonesia untuk induksi anastesi pada anjing. Studi ini menguji penggunaan obat Ket-A-Xyl® untuk kastrasi pada kucing. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian Ket-A-Xyl® terhadap parameter fisiologis kucing jantan domestik. Sebanyak 53 ekor kucing jantan domestik dengan berat badan berkisar 1,5 – 5,68 kg dipuasakan selama 8 jam kemudian ditimbang untuk menentukan dosis anestesi. Kucing diinjeksikan sediaan Ket-A-Xyl® secara intramuskular, kemudian diamati dan dicatat perubahan fisiologisnya. Demikian juga untuk onset dan durasi obat dicatat saat kucing memasuki stadium anestesi I hingga tahap recovery. Analisis data parameter fisiologis dilakukan menggunakan aplikasi SPSS dengan uji paired sample t-test. Kelompok yang dibandingkan dalam uji paired sample t-test tersebut adalah kelompok data fisiologis kucing pada tahap stadium III dengan tahap recovery. Hasil analisis data menunjukkan bahwa sediaan Ket-A-Xyl® menimbulkan efek yang signifikan terhadap frekuensi napas (p<0,05), tetapi tidak pada suhu dan frekuensi pulsus. Rata-rata onset dan durasi obat Ket-A-Xyl® menunjukkan waktu yang lebih singkat dibanding studi sebelumnya yang menggunakan obat atropin-ketamin-xylazin. Sediaan anastesi Ket-A-Xyl®  berpotensi  sebagai  obat anestetika yang baik  pada  kucing  domestik  karena  lebih  efisien,  onset dan durasi singkat, serta tidak menimbulkan respon fisiologis yang fatal selama penggunaannya.
Co-Authors Adi Tri Septyanto Agustina Dwi Wijayanti Agustina Dwi Wijayanti Agustina Dwi Wijayanti Agustina Dwi Wijayanti Agustina Dwi Wijayanti Anggi Muhtar Pratama Aria Ika Septana Claude Mona Airin Fitriana, Ida Maria Angelica Maryatmo Michael Haryadi Wibowo Slamet Rahardjo Tri Untari