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All Journal Journal of Dentistry Indonesia Jurnal Abdimas Kesehatan Terpadu
Riad, Anastasya Muna
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Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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The Effect of Parabiotic Reuterin on the Expression of Genes Involved in Candida albicans Biofilm Formation: An Ex vivo Study Riad, Anastasya Muna; Widyarman, Armelia Sari
Journal of Dentistry Indonesia Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

Candida albicans has a number of properties, including resistance to various antimicrobial agents, which allow it to survive in the root canals. Lactobacillus reuteri plays a role in maintaining oral health through interactions with the oral microbiome. L. reuteri has potential as a preventive and therapeutic agent against inflammatory diseases. Objective: The aim of this study was to investigate the effect of irrigation with a reuterin-containing solution on BCR1, ACE2, EFG1, and TEC1 gene expression in C. albicans root canal biofilms. Methods: L. reuteri was cultured in MRS broth and incubated anaerobically for 24 hours at 37°C. C. albicans was cultured in Sabouraud dextrose broth at 37°C for 48 hours. A total of 24 single-rooted premolar teeth were standardized and inoculated with C. albicans before irrigation with 50 μg/mL reuterin as a single, independent variable (Indonesian strain), 50 μg/mL reuterin Prodentis (a strain combination of L. reuteri DSM 17938 and L. reuteri ATCC PTA 5289), 2.5% sodium hypochlorite as positive control, and saline as negative control. A real-time quantitative polymerase chain reaction (RT-qPCR) assay was used to detect the expression of BCR1, ACE2, EFG1, and TEC1 in C. albicans root canal biofilms. Results: Reuterin significantly reduced the expression of BCR1 and ACE2 genes, which play a role in C. albicans biofilm formation, at the biofilm maturation stage (P < 0.05). Reuterin also affected the expression of the EFG1 and TEC1 genes, although the effect was not significant. Conclusion: A reuterin isolate of L. reuteri exhibits antibiofilm activity against the expression of C. albicans genes involved in biofilm formation. Reuterin has potential as an irrigation agent in the treatment of root canals. Further research is needed to shed light on the effectiveness of reuterin against the expression of genes that play important roles in the formation of C. albicans biofilms.
Pendidikan dan Pelatihan Mengenali Kelainan Rongga Mulut dan Cara Pencegahannya Dipankara, Jackson; Hadrian, Gary; Susanto, Shannon Winnie; Lidwina, Naomi; Riad, Anastasya Muna; Zen, Yuniar; Pakpahan, Alfred; Laksmiastuti, Sri Ratna; Poedjiastoeti, Wiwiek; Kurniawan, Vanesha Jane
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 2 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i2.21248

Abstract

The oral health promotion program is an effective way to enhance the community's ability to maintain their dental and oral health, provide access to information about dental and oral health, and guide the community toward adopting proper and correct behaviors regarding dental and oral health. The educational and training activities aim to increase the community’s knowledge about oral cavity disorders and their prevention. There were 82 participants from the Paguyuban Umat Senior Wihara Ekaya Arama (PUSA) community who took part in this activity. The training and education were conducted using PowerPoint presentations, videos, and posters as teaching tools. Based on the pre-test and post-test results, there was a noticeable increase in post-test scores, indicating an improvement in participants' knowledge after receiving education and training on oral cavity disorders and their prevention. The conclusion from the education and training activities provided to the PUSA community is that there was an increase in participants' knowledge. It is hoped that these educational and training activities will continue to benefit the community and enhance their ability to maintain dental and oral health.
Co-Authors Alfred Pakpahan, Alfred Dipankara, Jackson Hadrian, Gary Kurniawan, Vanesha Jane Lidwina, Naomi Sri Ratna Laksmiastuti Susanto, Shannon Winnie Widyarman, Armelia Sari Wiwiek Poedjiastoeti, Wiwiek Yuniar Zen