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All Journal Setrum : Sistem Kendali-Tenaga-elektronika-telekomunikasi-komputer Biology, Medicine, & Natural Product Chemistry IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Bakti Masyarakat Indonesia Jurnal ABDIMAS-HIP Pengabdian Kepada Masyarakat Jurnal Abdimas Kesehatan Terpadu AMMA : Jurnal Pengabdian Masyarakat Jurnal RAP (Riset Aktual Psikologi Universitas Negeri Padang)
Alfred Pakpahan
Faculty Of Dentistry, Universitas Trisakti, Jakarta, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Analisis Kinerja Model Pengontrol Ekson DNA Menggunakan Metode Model Hidden Markov Suhartati Agoes; Binti Solihah; Alfred Pakpahan
Setrum : Sistem Kendali-Tenaga-elektronika-telekomunikasi-komputer Vol 3, No 2 (2014): Edisi Desember 2014
Publisher : Fakultas Teknik Elektro - Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36055/setrum.v3i2.500

Abstract

urutan Deoxyribo asam nukleat (DNA) yang memiliki beberapa bagian ekson dalam urutan coding (cd) adalah bagian penting dalam proses biologis untuk menghasilkan protein. Tujuan dari penelitian ini adalah untuk mengontrol ekson DNA yang ada di CD dengan menggunakan Hidden Markov Model (HMM) sehingga protein yang dihasilkan tidak berubah. HMM metode memiliki parameter misalnya; negara, nilai keadaan transisi, negara emisi dasar dan algoritma yang digunakan untuk pelatihan dan proses pengujian. Nilai dari negara transisi secara acak berbagai ditentukan nilai antara 0 ~ 1. Pelaksanaan HMM di ekson kontroler memiliki struktur model 20-negara dan tes simulasi dilakukan dengan menggunakan nilai negara transisi dan jumlah urutan yang berbeda. Proses simulasi dengan struktur model 20-negara adalah menghasilkan nilai kinerja model dengan Koefisien Korelasi (CC) adalah 0,7571 dengan menggunakan 220 urutan. Penelitian ini meningkatkan nilai CC dengan cara mengelompokkan data dan hasilnya adalah 0,8808 untuk sub model dengan 69 urutan dan 0,8183 dengan 157 urutan.
Upaya Pencegahan COVID-19 melalui Peningkatan Pengetahuan Ibu mengenai PHBS di Lingkungan Keluarga pada Masa Pandemi Vidya Nursolihati; Annisaa Putri Ariyani; Lies Zubardiah; Alfred Pakpahan; Iqbal Zein Assyidiqie
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 7 No Special-1 (2022): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v7iSpecial-1.2500

Abstract

Until now, there is still no specific treatment that has proven effective for COVID-19, so prevention of the disease is the best way to break the chain of its transmission. This prevention effort requires participation from the entire community. However, it is not easy for people to participate due to a lack of understanding about the disease and awareness of clean-living behavior to prevent infection. Yayasan Rumah Ibu Indonesia (Rumaisa) is a foundation that focuses on increasing family resilience. One of the targets for clean and healthy living behavior (PHBS) is household/family; therefore, PHBS education and training in homes during the pandemic, especially for mothers, is a strategic way to mobilize and empower family members to practice clean and healthy behavior. This study aims to determine the effect of education and training of PHBS within families during the pandemic on mothers’’ knowledge in Yayasan Rumaisa. Participants’ level of knowledge is measured using a questionnaire given before and after the education and training, in the form of pre-test and post-test. From the results, it can be seen that the post-test scores were increased compared to the pre-test scores. An increase in participants’ knowledge of PHBS within families during the pandemic is expected to increase community participation in preventing disease transmission, especially COVID-19.
PELATIHAN PENGGUNAAN APLIKASI VIRTUAL LEARNING DI TPA ZIYAADATUL HASANAH Nazmia Kurniawati; Henry Candra; Yuli Kurnia Ningsih; Alfred Pakpahan; Tri Swasono Adi
Jurnal Bakti Masyarakat Indonesia Vol 5, No 1 (2022): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i1.17390

Abstract

Since the outbreak of the Corona Virus Disease (Covid-19) case in March 2020, the government issued a policy to conduct distance learning. Conditions that require learning activities to be carried out online caused the children to to use applications to carry out learning activities. One of the obstacles in implementing distance learning activities is the lack of technology mastery by parents and children. This causes children to have difficulty in learning the material provided by the school. In this activity, training was conducted to use virtual learning applications as learning media with the target of active parents and students in TPA Ziyaadatul Hasanah, Duren Sawit. With this training, it is hoped that parents and students can use virtual learning applications. So that parents can guide their sons and daughters in conducting distance learning activity and students are not left behind due to internet connection problems. From the results of the questionnaire given to participants to measure understanding of the training, participants gained increased knowledge about virtual learning applications as proofed by the increase in scores obtained from the pre-test and post-test results. In the pre-test the average score obtained by the participants was 8.3 while in the post-test the average score was 9.45 out of a scale of 10. In terms of satisfaction, the average score given by the participants was in the range of 4.1 to 4.75 and belongs to the very good range. Based on the results of discussions with participants, participants expected that there would be further activities related to their problems to be provided by the community service team.ABSTRAKSejak merebaknya kasus Corona Virus Disease (Covid-19) pada bulan Maret 2020, pemerintah mengeluarkan kebijakan untuk melakukan pembelajaran secara jarak jauh (PJJ). Kondisi yang mengharuskan kegiatan pembelajaran dilakukan secara daring menyebabkan anak-anak harus menggunakan aplikasi untuk melakukan kegiatan pembelajaran. Salah satu kendala dalam pelaksanaan kegiatan PJJ adalah kurangnya penguasaan teknologi oleh orang tua dan anak. Hal tersebut menyebabkan anak-anak kesulitan dalam mempelajari materi yang diberikan. Pada kegiatan ini dilakukan pelatihan penggunaan aplikasi virtual learning sebagai media pembelajaran dengan sasaran orang tua dan siswa yang aktif di TPA Ziyaadatul Hasanah, Duren Sawit. Dengan adanya pelatihan ini diharapkan orang tua dan siswa dapat menggunakan aplikasi virtual learning. Sehingga orang tua  dapat membimbing putra putrinya dalam PJJ dan siswa tidak tertinggal materi karena masalah koneksi internet. Dari hasil kuesioner yang diberikan kepada peserta untuk mengukur pemahaman terhadap pelatihan yang diberikan, peserta mendapatkan peningkatan pengetahuan mengenai aplikasi virtual learning yang dibuktikan dengan peningkatan nilai yang didapatkan dari hasil pre-test dan post-test. Pada pre-test nilai rata-rata yang didapatkan peserta adalah 8.3 sedangkan pada post-test rata-rata nilainya adalah 9.45 dari skala 10. Sementara dari sisi kepuasan, rata-rata skor yang diberikan oleh peserta berada pada rentang 4.1 hingga 4.75 dan termasuk ke dalam rentang sangat baik. Berdasarkan hasil diskusi dengan peserta, warga mengharapkan adanya kegiatan lanjutan dari pelatihan virtual learning yang dilakukan pada periode PkM ini.
PELATIHAN METODE BERBAGI KONEKSI INTERNET DENGAN SATU KUOTA PAKET DATA DALAM SATU KELUARGA UNTUK PEMBELAJARAN JARAK JAUH DI MASA PANDEMI Henry Candra; Suhartati Agoes; R. Deiny Mardian; Alfred Pakpahan
Jurnal Bakti Masyarakat Indonesia Vol 4, No 2 (2021): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i2.11351

Abstract

The outbreak of the Covid-19 virus pandemic forced the government to enforce rules for studying from home. Although many online learning facilities is available, the access requires adequate internet connection and supporting devices. This limited internet connection is a problem often faced by the students. The Internet connection can be made using a data package from a smartphone; however, lack of knowledge in using the features in a smartphone causes difficulties to share access and the solution taken is each smartphone is subscribed to an individual data packet. The lack of knowledge on sharing internet access using smartphones is the issue faced by teachers and parents at TPA Ziyaadatul Hasanah, Duren Sawit, East Jakarta. There is an alternative solution to overcome the problem by using Tethering and Hotspot technology to share the internet access using a single data package. Its application requires correct procedures so that the internet connection can be shared properly. This community service activity provided knowledge and skills training in using internet connection sharing methods using tethering and hotspot technology and how to solve problems that arise for teachers and parents of students at TPA Ziyaadatul Hasanah with the target to increase their knowledge and skills in sharing the internet access using smartphones. Based on the survey, the knowledge of participants increased after the training, with an average increase of 17.6% before training to 41.2% for participants with good scores. In addition, they can also solve problems that arise when implementing the method given in the trainingABSTRAK:Terjadinya pandemi virus Covid-19 memaksa pemerintah untuk memberlakukan aturan belajar dari rumah. Meskipun fasilitas belajar daring (online) begitu banyak tetapi aksesnya memerlukan fasilitas koneksi internet yang cukup dengan perangkat yang memadai. Keterbatasan koneksi internet tersebut menjadi permasalahan yang sering dihadapi oleh para murid sekolah. Koneksi internet dapat dilakukan dengan menggunakan paket data dari telepon pintar akan tetapi keterbatasan pengetahuan dalam penggunaan fitur dari telepon pintar menyebabkan kesulitan untuk dapat berbagi akses dan solusi yang diambil biasanya adalah masing-masing telepon pintar menggunakan paket data individual. Keterbatasan pengetahuan tentang berbagi akses internet menggunakan telepon pintar tersebut merupakan masalah yang dihadapi oleh guru dan orang tua murid di TPA Ziyaadatul Hasanah, Duren Sawit, Jakarta Timur. Ada solusi altenatif yang dapat dilakukan untuk mengatasi masalah tersebut yaitu dengan menggunakan teknologi Hotspot dan Tethering untuk berbagi internet dengan satu kuota paket data. Penerapannya memerlukan prosedur dan tahapan yang benar agar koneksi internet dapat berjalan dengan baik, oleh karena itu, pada program kegiatan pengabdian kepada masyarakat ini diberikan pengetahuan dan pelatihan ketrampilan dalam melakukan metode berbagi koneksi internet menggunakan teknologi tethering dan hotspot serta bagaimana mengatasi permasalahan yang timbul kepada guru dan orang tua murid di TPA Ziyaadatul Hasanah dengan target untuk meningkatkan pengetahuan dan ketrampilan para peserta pelatihan dalam berbagi internet menggunakan telepon pintar. Berdasarkan hasil survei diperoleh bahwa pengetahuan dari peserta meningkat setelah mereka mengikuti pelatihan, dengan peningkatan sebesar rata-rata 17,6% sebelum pelatihan menjadi 41,2% untuk peserta dengan nilai baik. Selain itu mereka juga dapat mengatasi masalah yang timbul ketika menerapkan metode yang diajarkan
PELATIHAN PENGGUNAAN APLIKASI VIRTUAL LEARNING DI TPA ZIYAADATUL HASANAH Nazmia Kurniawati; Henry Candra; Yuli Kurnia Ningsih; Alfred Pakpahan; Tri Swasono Adi
Jurnal Bakti Masyarakat Indonesia Vol. 5 No. 1 (2022): Jurnal Bakti Masyarakat Indonesia
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Tarumanagara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24912/jbmi.v4i1.17390

Abstract

Since the outbreak of the Corona Virus Disease (Covid-19) case in March 2020, the government issued a policy to conduct distance learning. Conditions that require learning activities to be carried out online caused the children to to use applications to carry out learning activities. One of the obstacles in implementing distance learning activities is the lack of technology mastery by parents and children. This causes children to have difficulty in learning the material provided by the school. In this activity, training was conducted to use virtual learning applications as learning media with the target of active parents and students in TPA Ziyaadatul Hasanah, Duren Sawit. With this training, it is hoped that parents and students can use virtual learning applications. So that parents can guide their sons and daughters in conducting distance learning activity and students are not left behind due to internet connection problems. From the results of the questionnaire given to participants to measure understanding of the training, participants gained increased knowledge about virtual learning applications as proofed by the increase in scores obtained from the pre-test and post-test results. In the pre-test the average score obtained by the participants was 8.3 while in the post-test the average score was 9.45 out of a scale of 10. In terms of satisfaction, the average score given by the participants was in the range of 4.1 to 4.75 and belongs to the very good range. Based on the results of discussions with participants, participants expected that there would be further activities related to their problems to be provided by the community service team.ABSTRAKSejak merebaknya kasus Corona Virus Disease (Covid-19) pada bulan Maret 2020, pemerintah mengeluarkan kebijakan untuk melakukan pembelajaran secara jarak jauh (PJJ). Kondisi yang mengharuskan kegiatan pembelajaran dilakukan secara daring menyebabkan anak-anak harus menggunakan aplikasi untuk melakukan kegiatan pembelajaran. Salah satu kendala dalam pelaksanaan kegiatan PJJ adalah kurangnya penguasaan teknologi oleh orang tua dan anak. Hal tersebut menyebabkan anak-anak kesulitan dalam mempelajari materi yang diberikan. Pada kegiatan ini dilakukan pelatihan penggunaan aplikasi virtual learning sebagai media pembelajaran dengan sasaran orang tua dan siswa yang aktif di TPA Ziyaadatul Hasanah, Duren Sawit. Dengan adanya pelatihan ini diharapkan orang tua dan siswa dapat menggunakan aplikasi virtual learning. Sehingga orang tua  dapat membimbing putra putrinya dalam PJJ dan siswa tidak tertinggal materi karena masalah koneksi internet. Dari hasil kuesioner yang diberikan kepada peserta untuk mengukur pemahaman terhadap pelatihan yang diberikan, peserta mendapatkan peningkatan pengetahuan mengenai aplikasi virtual learning yang dibuktikan dengan peningkatan nilai yang didapatkan dari hasil pre-test dan post-test. Pada pre-test nilai rata-rata yang didapatkan peserta adalah 8.3 sedangkan pada post-test rata-rata nilainya adalah 9.45 dari skala 10. Sementara dari sisi kepuasan, rata-rata skor yang diberikan oleh peserta berada pada rentang 4.1 hingga 4.75 dan termasuk ke dalam rentang sangat baik. Berdasarkan hasil diskusi dengan peserta, warga mengharapkan adanya kegiatan lanjutan dari pelatihan virtual learning yang dilakukan pada periode PkM ini.
Cytotoxic test of different solvents of soursop (Annona muricata) leaf extract against HSC-3 cell line Areta Vania Bhanuwati; Alfred Pakpahan
Dental Journal Vol. 55 No. 3 (2022): September 2022
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v55.i3.p130-136

Abstract

Background: Soursop (Annona muricata) leaves have been researched extensively and found to have anticancer properties. The use of soursop as an anticancer treatment is increasingly popular due to its selective cytotoxic activity by acetogenins. The polarity of the extract solvent contributes to the biological activity of the plant, namely cytotoxicity. Purpose: To determine the cytotoxicity of A. muricata leaf extract with ethanol, ethyl acetate and hexane fractions against human oral squamous carcinoma (HSC-3) cell lines. Methods: This experimental laboratory study consisted of twenty four treatment groups tested against the HSC-3 cell line. The ethanol, ethyl acetate and hexane fractions of A. muricata leaves were administered to seven different concentrations, namely 0.3 μg/mL, 3 μg/mL, 25 μg/mL, 50 μg/mL, 100 μg/mL, 150 μg/mL and 300 μg/mL. The control group consisted of three groups: negative control, solvent control and positive control. The percentage of cell viability was calculated by absorbent enzyme-linked immunosorbent assay (ELISA) reader. The cytotoxicity of A. muricata leaf extract against HSC-3 cells was determined by cell counting kit-8 (CCK-8) assay and expressed by IC50 value. The results were analysed using one-way analysis of variance (ANOVA) followed by Tukey’s honestly significant difference (HSD). Results: The results show that the leaf extracts of A. muricata are moderately cytotoxic to HSC-3 cells. The highest cytotoxic activity was found in the ethyl acetate extract with an IC50 value of 76.66 μg/mL – making it the best solvent – then hexane (IC50: 84.14 μg), then ethanol (IC50: 101.32 μg/mL). Statistical analysis using one-way ANOVA and Tukey’s HSD is considered significant p < 0.001. Conclusion: Ethanol, ethyl acetate and hexane fractions of A. muricata leaf extract are moderately cytotoxic, with IC50 values in the range of 21–200 μg/mL.
Pemberdayaan Guru dan Orang Tua tentang Penanganan Kegawatdaruratan Rongga Mulut Anak Pra Sekolah Ricky Anggara Putranto; Tiarma Talenta Theresia; Johan Arief Budiman; Tien Suwartini; Alfred Pakpahan
Jurnal ABDIMAS-HIP Pengabdian Kepada Masyarakat Vol 4 No 2 (2023): Jurnal ABDIMAS-HIP Pengabdian Kepada Masyarakat
Publisher : LPPM Akbid Harapan Ibu Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37402/abdimaship.vol4.iss2.242

Abstract

Oral emergencies are trauma involving the teeth and the supporting structures of the teeth. This trauma can be in the form of open wounds, broken teeth and loose teeth. During the COVID-19 pandemic, mothers had more time to spend with their children, so they were expected to be able to provide quick and appropriate action if their children experienced trauma to their teeth. This empowerment activity aims to increase knowledge and skills in handling oral emergencies. This Community Service activity was carried out by the Trisakti University Faculty of Dentistry Team for Teachers and Parents of Kindergarten Al Hidayah 2 Kelapa Gading on December 1, 2022. Counseling and training were carried out offline using presentations on open wounds, broken teeth loose teeth, and first aid that can be done at home. Training and counseling was carried out to 46 participants for 60 minutes. Participants' knowledge was assessed by pre-test and post-test using Google Form. From the results obtained, it was seen that there was an increase in the knowledge of the counseling participants by 13.5% (mean pre-test score was 28.9 ± 14.6 and mean post-test score was 68 ± 15.3).
Pendidikan dan Pelatihan Mengenali Kelainan Rongga Mulut dan Cara Pencegahannya Dipankara, Jackson; Hadrian, Gary; Susanto, Shannon Winnie; Lidwina, Naomi; Riad, Anastasya Muna; Zen, Yuniar; Pakpahan, Alfred; Laksmiastuti, Sri Ratna; Poedjiastoeti, Wiwiek; Kurniawan, Vanesha Jane
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 2 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i2.21248

Abstract

The oral health promotion program is an effective way to enhance the community's ability to maintain their dental and oral health, provide access to information about dental and oral health, and guide the community toward adopting proper and correct behaviors regarding dental and oral health. The educational and training activities aim to increase the community’s knowledge about oral cavity disorders and their prevention. There were 82 participants from the Paguyuban Umat Senior Wihara Ekaya Arama (PUSA) community who took part in this activity. The training and education were conducted using PowerPoint presentations, videos, and posters as teaching tools. Based on the pre-test and post-test results, there was a noticeable increase in post-test scores, indicating an improvement in participants' knowledge after receiving education and training on oral cavity disorders and their prevention. The conclusion from the education and training activities provided to the PUSA community is that there was an increase in participants' knowledge. It is hoped that these educational and training activities will continue to benefit the community and enhance their ability to maintain dental and oral health.
Effects of Sambiloto (Andrographis paniculata) Extract and Spirulina (Spirulina platensis) Administration on Ki-67 Protein Expression in the Colon Epithelial Cells of Plasmodium berghei-infected Mice Kusmardi, Kusmardi; Ariffandi, Bagas; Lubis, Nadar Sukri; Lestari, Tri Wahyuni; Intan, Putri Reno; Pakpahan, Alfred
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1737

Abstract

BACKGROUND: Malaria remains a global health concern and an endemic disease in Indonesia. Sambiloto (Andrographis paniculata) and spirulina (Spirulina platensis) are two potential antimalarial agents which act as antioxidants and antiinflammatories that can suppress morbidities during chronic inflammation in malaria, such as proliferation in the colon. This study aims to investigate the effects of A. paniculata extract and S. platensis administration on Ki-67 expression in medial colon epithelial cells of Plasmodium berghei-infected mice measured by H-score.METHODS: Thirty P. berghei-infected male Swiss-Webster mice were divided into five groups: negative controls (carboxymethyl cellulose/CMC); positive controls (dihydroartemisinin-piperaquine/DHP); A. paniculata extract alone (AP); A. paniculata extract in combination with S. platensis extract (AP+ES); and with S. platensis powder (AP+PS). All mice were infected with P. berghei on day 0. The treatment for each group were given 3 days before infection (D-3) until the day of infection (D0) for 28 days after infection. Colon tissues were processed with immunohistochemistry to detect Ki-67.RESULTS: A difference in Ki-67 expression was observed among the groups (p<0.01). The mean H-score for the CMC control group is 135.503±6.723. The lowest level of Ki-67 expression was observed in the AP+PS group (H-score= 110.941±7.079). AP group did not show a significant difference from the CMC group (p=0.514) and neither did the AP+ES group (p=0.234).CONCLUSION: In conclusion, administration of A. paniculata extract and S. platensis powder lowers Ki-67 expression in medial colon epithelial cells of P. berghei-infected mice.KEYWORDS: malaria, spirulina, Ki-67, Andrographis paniculata, Spirulina platensis
Inositol Hexakisphosphate (InsP₆) Induces Apoptosis via Caspase-Dependent Pathways: Molecular Docking Insights Ferry Sandra; Dewi Ranggaini; Johni Halim; Alfred Pakpahan; Visi Endah Pratitis; Kyung Hoon Lee
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3810

Abstract

BACKGROUND: Inositol hexakisphosphate (InsP₆) exhibits anticancer activity, especially by inducing intrinsic and extrinsic apoptotic pathways. However, there is still no molecular docking evidence that directly examines InsP₆ interactions with either upstream or downstream apoptotic regulators. Therefore, the current study was conducted to investigate the molecular docking of InsP₆ to caspases as upstream/downstream apoptotic regulators.METHODS: Ligands including InsP₆, InsP₅, InsP₄, histone deacetylase inhibitor, and caspase inhibitors were retrieved from PubChem, while target proteins (histone, caspase-8, caspase-2, and caspase-3) were obtained from the Protein Data Bank. Ligand toxicity was predicted using ProTox-3.0, and physicochemical properties were analyzed with SwissADME. Ligand structures were energy-minimized using PyRx with the Universal Force Field, while proteins were prepared by removing water molecules and non-essential heteroatoms in BIOVIA Discovery Studio. Molecular docking was conducted using CB-Dock 2.0, with binding poses selected based on the lowest Vina score, and ligand–protein interactions were visualized in Discovery Studio.RESULTS: Molecular docking results showed that InsP₆ bound strongly to histone, caspase-8, caspase-2, and caspase-3 with affinities comparable to reference inhibitors, forming multiple hydrogen bonds with key active-site residues. InsP₆, InsP₅, and InsP₄ exhibited several similar binding sites to caspase-3, with only minor differences in binding affinity.CONCLUSION: InsP₆ shows strong binding to histone, caspase-8, caspase-2, and caspase-3 based on in silico results, supporting its role in inducing both extrinsic and intrinsic apoptotic pathways. Taken together, InsP₆ could be a potential inducer of apoptosis in cancer cells.KEYWORDS: cancer, apoptosis, InsP₆, InsP₅, InsP₄, caspase, in silico, molecular docking
Co-Authors Abrijanto Abrijanto Agita Tunjungsari Ananda Shelly Melyda Aprilia Puspita Sulistia Areta Vania Bhanuwati Ariffandi, Bagas Ariyani, Annisaa Putri Arneliza Arneliza Binti Solihah David Tjahyadi Dewi Priandini Dewi Ranggaini Dewi Ranggaini Dipankara, Jackson Edy Parwanto Ferry Sandra Hadrian, Gary Haryo Ganeca Widyatama Henry Candra Iqbal Zein Assyidiqie Johan Arief Budiman Johni Halim Johni Halim Komariah Komariah Kuncono Teguh Yunanto Kurnia Ningsih, Yuli Kurniawan, Vanesha Jane Kurniawati, Nazmia Kusmardi , Kyung Hoon Lee Lestari, Tri Wahyuni Lidwina, Naomi Lies Zubardiah Lubis, Nadar Sukri Nursolihati, Vidya Putri Reno Intan R. Deiny Mardian Rahmi Amtha Reza Aditya Digambiro Rezky Anggraeni Riad, Anastasya Muna Ricky Anggara Putranto Rudy Sutadi Sisca Sisca Sri Ratna Laksmiastuti Suhartati Agoes Suhartati Agoes Susanto, Shannon Winnie Suwartini, Tien Tiarma Talenta Theresia Tri swasono adi Visi Endah Pratitis Wiwiek Poedjiastoeti, Wiwiek Yuniar Zen