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All Journal IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) The Indonesian Biomedical Journal Jurnal Abdimas Kesehatan Terpadu AMMA : Jurnal Pengabdian Masyarakat
Alfred Pakpahan, Alfred
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Tingkat Pengetahuan Kecacingan Di Desa Sukasari Pandeglang Tjahyadi, David; Parwanto, Edy; Ganeca Widyatama, Haryo; Sisca, Sisca; Aditya Digambiro, Reza; Pakpahan, Alfred
AMMA : Jurnal Pengabdian Masyarakat Vol. 3 No. 6 : Juli (2024): AMMA : Jurnal Pengabdian Masyarakat
Publisher : CV. Multi Kreasi Media

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Worm disease still a serious problem and is second health problem in Indonesia come after malnutrition. Cumulative worms in human body can cause loss of substances that are useful for growth. The Filariasis and Worm Worms Sub-Directorate of P2PL stated that the prevalence of worm diseases in Pandeglang Regency was 58.3%. Various worm larvae were found when examining soil samples taken at the homes of residents of Sukasari, Pulosari, Pandeglang villages. The aim of this community service (PKM) is to increase knowledge about worm diseases. This Community Service Method (PKM) uses the counseling or lecture method by the presenter. Before and after counseling, residents were given a questionnaire to measure their level of knowledge about worm diseases. Residents' knowledge increased after being given counseling and it is hoped that this can help the community to prevent the emergence of worm diseases in the future.
Pendidikan dan Pelatihan Mengenali Kelainan Rongga Mulut dan Cara Pencegahannya Dipankara, Jackson; Hadrian, Gary; Susanto, Shannon Winnie; Lidwina, Naomi; Riad, Anastasya Muna; Zen, Yuniar; Pakpahan, Alfred; Laksmiastuti, Sri Ratna; Poedjiastoeti, Wiwiek; Kurniawan, Vanesha Jane
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 3 No. 2 (2024): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v3i2.21248

Abstract

The oral health promotion program is an effective way to enhance the community's ability to maintain their dental and oral health, provide access to information about dental and oral health, and guide the community toward adopting proper and correct behaviors regarding dental and oral health. The educational and training activities aim to increase the community’s knowledge about oral cavity disorders and their prevention. There were 82 participants from the Paguyuban Umat Senior Wihara Ekaya Arama (PUSA) community who took part in this activity. The training and education were conducted using PowerPoint presentations, videos, and posters as teaching tools. Based on the pre-test and post-test results, there was a noticeable increase in post-test scores, indicating an improvement in participants' knowledge after receiving education and training on oral cavity disorders and their prevention. The conclusion from the education and training activities provided to the PUSA community is that there was an increase in participants' knowledge. It is hoped that these educational and training activities will continue to benefit the community and enhance their ability to maintain dental and oral health.
Inositol Hexakisphosphate (InsP₆) Induces Apoptosis via Caspase-Dependent Pathways: Molecular Docking Insights Sandra, Ferry; Ranggaini, Dewi; Halim, Johni; Pakpahan, Alfred; Pratitis, Visi Endah; Lee, Kyung Hoon
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3810

Abstract

BACKGROUND: Inositol hexakisphosphate (InsP₆) exhibits anticancer activity, especially by inducing intrinsic and extrinsic apoptotic pathways. However, there is still no molecular docking evidence that directly examines InsP₆ interactions with either upstream or downstream apoptotic regulators. Therefore, the current study was conducted to investigate the molecular docking of InsP₆ to caspases as upstream/downstream apoptotic regulators.METHODS: Ligands including InsP₆, InsP₅, InsP₄, histone deacetylase inhibitor, and caspase inhibitors were retrieved from PubChem, while target proteins (histone, caspase-8, caspase-2, and caspase-3) were obtained from the Protein Data Bank. Ligand toxicity was predicted using ProTox-3.0, and physicochemical properties were analyzed with SwissADME. Ligand structures were energy-minimized using PyRx with the Universal Force Field, while proteins were prepared by removing water molecules and non-essential heteroatoms in BIOVIA Discovery Studio. Molecular docking was conducted using CB-Dock 2.0, with binding poses selected based on the lowest Vina score, and ligand–protein interactions were visualized in Discovery Studio.RESULTS: Molecular docking results showed that InsP₆ bound strongly to histone, caspase-8, caspase-2, and caspase-3 with affinities comparable to reference inhibitors, forming multiple hydrogen bonds with key active-site residues. InsP₆, InsP₅, and InsP₄ exhibited several similar binding sites to caspase-3, with only minor differences in binding affinity.CONCLUSION: InsP₆ shows strong binding to histone, caspase-8, caspase-2, and caspase-3 based on in silico results, supporting its role in inducing both extrinsic and intrinsic apoptotic pathways. Taken together, InsP₆ could be a potential inducer of apoptosis in cancer cells.KEYWORDS: cancer, apoptosis, InsP₆, InsP₅, InsP₄, caspase, in silico, molecular docking
Effects of Sambiloto (Andrographis paniculata) Extract and Spirulina (Spirulina platensis) Administration on Ki-67 Protein Expression in the Colon Epithelial Cells of Plasmodium berghei-infected Mice Kusmardi, Kusmardi; Ariffandi, Bagas; Lubis, Nadar Sukri; Lestari, Tri Wahyuni; Intan, Putri Reno; Pakpahan, Alfred
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1737

Abstract

BACKGROUND: Malaria remains a global health concern and an endemic disease in Indonesia. Sambiloto (Andrographis paniculata) and spirulina (Spirulina platensis) are two potential antimalarial agents which act as antioxidants and antiinflammatories that can suppress morbidities during chronic inflammation in malaria, such as proliferation in the colon. This study aims to investigate the effects of A. paniculata extract and S. platensis administration on Ki-67 expression in medial colon epithelial cells of Plasmodium berghei-infected mice measured by H-score.METHODS: Thirty P. berghei-infected male Swiss-Webster mice were divided into five groups: negative controls (carboxymethyl cellulose/CMC); positive controls (dihydroartemisinin-piperaquine/DHP); A. paniculata extract alone (AP); A. paniculata extract in combination with S. platensis extract (AP+ES); and with S. platensis powder (AP+PS). All mice were infected with P. berghei on day 0. The treatment for each group were given 3 days before infection (D-3) until the day of infection (D0) for 28 days after infection. Colon tissues were processed with immunohistochemistry to detect Ki-67.RESULTS: A difference in Ki-67 expression was observed among the groups (p<0.01). The mean H-score for the CMC control group is 135.503±6.723. The lowest level of Ki-67 expression was observed in the AP+PS group (H-score= 110.941±7.079). AP group did not show a significant difference from the CMC group (p=0.514) and neither did the AP+ES group (p=0.234).CONCLUSION: In conclusion, administration of A. paniculata extract and S. platensis powder lowers Ki-67 expression in medial colon epithelial cells of P. berghei-infected mice.KEYWORDS: malaria, spirulina, Ki-67, Andrographis paniculata, Spirulina platensis
Antibacterial Activity of Nanocomposite Chitosan-Silver Nanoparticle with Cymbopogon citratus Extract as a Bioreductor Against Staphylococcus aureus Anggraeni, Rezky; Melyda, Ananda Shelly; Pakpahan, Alfred; Ranggaini, Dewi; Halim, Johni; Komariah, Komariah
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 2 (2025): June 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i2.112

Abstract

Nanocomposites are materials formed by combining two components, one or both of which are on the nanometer scale. The nanocomposite in this study is a combination of chitosan and silver nanoparticles produced through the synthesis of silver nitrate using Cymbopogon citratus extract. Silver nanoparticles have antibacterial abilities that can be utilized to overcome various diseases. However, their antibacterial properties may be reduced due to the tendency of silver nanoparticles to agglomerate. This can be overcome by the addition of chitosan as a stabilizing agent to prevent agglomeration and maintain the antibacterial effectiveness of silver nanoparticles. This study aims to evaluate the antibacterial activity of a nanocomposite formed by combining chitosan and silver nanoparticles synthesized using Cymbopogon citratus extract against Staphylococcus aureus through the diffusion method. The samples used included nanocomposites at concentrations of 6.25 mg/mL, 12.5 mg/mL, 15 mg/mL, 25 mg/mL, and 50 mg/mL, amoxicillin as a positive control, Acetic acid, and distilled water as negative controls. The results of antibacterial activity testing showed that all nanocomposite test concentrations had the ability to inhibit the growth of Staphylococcus aureus as evidenced by the formation of an inhibition zone around the disc paper. However, the highest antibacterial activity shown by the nanocomposites was still lower compared to the antibacterial activity of amoxicillin Keywords: Antibacterial activity, Chitosan, Cymbopogon citratus, Silver nanoparticles, Staphylococcus aureus
Co-Authors Anggraeni, Rezky Ariffandi, Bagas David Tjahyadi Dipankara, Jackson Ferry Sandra Ganeca Widyatama, Haryo Hadrian, Gary Halim, Johni Komariah Komariah Kurniawan, Vanesha Jane Kusmardi Kusmardi Lee, Kyung Hoon Lestari, Tri Wahyuni Lidwina, Naomi Lubis, Nadar Sukri Melyda, Ananda Shelly Parwanto, Edy Pratitis, Visi Endah Putri Reno Intan Ranggaini, Dewi Reza Aditya Digambiro Riad, Anastasya Muna Sisca Sisca, Sisca Sri Ratna Laksmiastuti Susanto, Shannon Winnie Wiwiek Poedjiastoeti, Wiwiek Yuniar Zen