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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Hortikultura Jurnal Ilmu Ternak Veteriner Animal Production : Indonesian Journal of Animal Production Societa: Jurnal Ilmu-Ilmu Agribisnis JURNAL PENDIDIKAN TAMBUSAI Applied Engineering and Technology
S Anwar
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Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences Veterinary

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Journal : Jurnal Ilmu Ternak Veteriner

 1 
Genetic Polymorphism of SCD1 Gene of Holstein-Friesian Cows in Indonesia Ari Sulistyo Wulandari; HD Rahayu; SD Volkandari; N Herlina; S Anwar; Y Irnidayanti
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 2 (2019): JUNE 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.768 KB) | DOI: 10.14334/jitv.v24i2.1905

Abstract

Stearoyl-Coenzyme A desaturase 1 (SCD1) belongs to the fatty acid family of desaturases. In lactating ruminants, the SCD1 protein is highly expressed in the mammary gland and is relevant for the fatty acid composition of milk and dairy products. Polymorphism of SCD1 gene in Holstein-Friesian (HF) cows could be used as a basis of molecular selection of cattle in order to increase their productivity. The aim of this study was to investigate the polymorphism of SCD1 gene of Holstein-Friesian cows in Indonesia. A total of 162 blood samples of HF cows were collected from four different locations i.e. Bogor, Sukabumi, Tasikmalaya and Enrekang districts. Genotyping of SCD1 gene used PCR-RFLP method with NcoI restriction enzyme. The result showed that three genotypes (AA, AV and VV) and two alleles (A and V) have successfully found and polymorphic. A allele was dominant in all populations (0.63) and in Hardy Weinberg Equilibrium. The highest A allele was found in Sukabumi (0.78) and the lowest was in Bogor (0.55). Heterozigosity observed and expected reached 0.471 and 0.470, respectively. In conclusion, genetic polymorphism was found in all population with dominant of A allele. This finding can be used as a early genetic information of Holstein-Friesian cattle in Indonesia and to build breeding strategy for improving of productivity especially improving of healthy fat milk. 
Identification of GH|MspI and GHR|AluI Gene Polymorphism and its Association with Calf Birth Weight of Grati-PO Cattle Hartati Hartati; BDP Soewandi; AAR Hapsari; S Anwar; D Pamungkas
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 2 (2019): JUNE 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.616 KB) | DOI: 10.14334/jitv.v24i2.1939

Abstract

Calf birth weight (CBW) is one of the important selection criteria to predict mature body weight and for calving ease in beef cattle. The GH and GHR genes are considered as candidate genes responsible for growth traits in cattle. The objectives of this study were to identify the polymorphism of GH|MspI and GHR|AluI genes and its association with CBW in Grati-PO cattle. A total of 186 Grati-PO cattle raised by BCRS from May to December 2017. Genomic DNA were isolated from whole blood and used in genotyping analysis using the PCR-RFLP method. The result showed that the average of CBW of Grati-PO cattle in present study was 25.58±3.31 kg. There was no statistical difference for CBW between male and female. The genotype frequency of CC, CT, and TT of GH gene were 1.1, 18.8 and 80.1 %, respectively and allele frequency of C and T of the GH gene were 0.105 and 0.895, respectively. While the genotype frequency of AA, AG, and GG of GHR gene were 66.1, 25.3 and 8.6 %, respectively, and allele frequency of A and G of GHR gene were 0.788 and 0.212, respectively. It concluded that both GH|MspI and GHR|AluI gene are polymorphic but not significantly associated with CBW in Grati-PO cattle.
Co-Authors - Martias . Khalil AAR Hapsari Anas D Susila Anas Dinurrohman Susila Ari Sulistyo Wulandari BDP Soewandi CH Prayitno, CH CI Sutrisno, CI D Pamungkas Desi Hernita Dewi, M A ED Purbajanti, ED F Kusmiyati, F Hartati Hartati HD Rahayu Irawan, A Larasati, P D Mulya, M F Munasik Munasik N Herlina Nurfatima, I Pangestu, A R Hidayati R Poerwanto Rhoedy Poerwanto Rih Laksmi Utpalasari S Widyati, S SD Volkandari Setiawan, AB Y Irnidayanti