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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biotropika
Dhike Ardhian
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Kandungan Oksalat Umbi Porang (Amorphophallus muelleri Blume) Hasil Penanaman dengan Perlakuan Pupuk P dan K Ardhian, Dhike; Indriyani, Serafinah
Biotropika: Journal of Tropical Biology Vol 1, No 2 (2013)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.723 KB)

Abstract

Porang (Amorphophallus muelleri Blume) merupakan salah satu tanaman bergizi yang memiliki asam oksalat yang berdampak negatif bagi kesehatan manusia. Pupuk P dan K diduga dapat digunakan untuk mereduksi kandungan oksalat pada porang. Tujuan penelitian ini adalah untuk mengetahui  pengaruh pemberian pupuk P dan K secara tunggal maupun kombinasi pada kandungan oksalat umbi porang dan mengetahui jenis dan dosis pupuk yang dapat mereduksi kandungan oksalat. Perlakuan terdiri dari kombinasi empat dosis pupuk K dan empat dosis pupuk P dengan dosis yang sama yaitu 0;2,16;4,32;dan 6,36 g/12 kg tanah yang diaplikasikan ke tanaman porang yang ditumbuhkan hingga empat bulan. Oksalat terlarut dianalisis dengan volumetri dan oksalat tidak terlarut dianalisis dengan spektrofotometri (AAS). Analisis statistik menggunakan ANOVA dilanjutkan uji Tukey α = 5%. Kombinasi pupuk P dan K serta pupuk P tunggal tidak mempengaruhi kandungan oksalat terlarut, tidak terlarut, dan total pada umbi porang. Pemberian pupuk K tunggal pada dosis 2,16 g/12 kg tanah mampu menurunkan kandungan oksalat total sebanyak 34,3 %. Dengan demikian dosis Pupuk K ini berpotensi untuk menurunkan kandungan oksalat total umbi porang.   Kata kunci : oksalat, pupuk K, pupuk P, spektrofotometri, volumetri
Assessing Exogenous L-Glutamine and PEG Influence on Soybean Adventitious Roots Culture: Lateral Root Morphology and Amino Acid Profiles: L-Glutamine and PEG Influence on Adventitious Roots Culture Ardhian, Dhike; Mastuti, Retno; Nahampun, Hartinio Natalia
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.01

Abstract

Soybean (Glycine max L. Merr.) plays an important role in global agriculture and provides a primary source of plant nutrition, notably amino acids. However, tropical climates often result in lower amino acid levels than subtropical climates. This study explored the effects of L-glutamine and PEG on enhancing the amino acid content in in vitro soybean adventitious root. In this study, in vitro adventitious roots from the Detap1 soybean variety were treated in a liquid medium with various concentrations of L-glutamine (0, 1, 2, 4, 8, and 16 g.L-1) or PEG (0, 10, 20, 40, 80, and 160 g.L-1) for 8 days. The root biomass was quantified, and the amino acid profile was analyzed using HPLC. L-glutamine or PEG treatments significantly reduced root biomass by inhibiting lateral root initiation. Meanwhile, 20 amino acids were detected in the control and L-glutamine or PEG treatments. The application of a 16 g.L-1 L-glutamine significantly increased amino acid content. PEG treatments also had similar results in root growth inhibition and increased amino acid accumulation. Multivariate analysis showed significant separation between the control and treatment groups, with glutamine playing a crucial role in the group separation. This study demonstrates the potential of in vitro technology to improve amino acid production through adventitious root culture, providing insights into stress responses and metabolic regulations
Co-Authors Nahampun, Hartinio Natalia Retno Mastuti Serafinah Indriyani