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S arjito
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APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) S arjito; Nestin E.W. Ningrum; Ocky Karna Radjasa; Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A molecular-based study was conducted to estimate the richness of the Vibrio on  tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates  (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed  that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively.  The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore  the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on  tiger shrimp.
THE WHITE SPOT SYNDROME VIRUS (WSSV) LOAD IN Dendronereis spp. D esrina; S arjito; Alfabetian Harjuno Condro Haditomo; Diana Chilmawati
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.812 KB)

Abstract

The white spot syndrome virus (WSSV), the causative agent of White Spot Syndrome Disease (WSSD), is a major shrimp pathogen in Indonesia. Dendronereis spp. is a ubiquitous Polychaetes and natural food of shrimp raised in brackishwater pond in Indonesia. The objective of this research is to determine the occurrence of WSSV and the viral load in Dendronereis spp. obtained from the shrimp pond. Dendronereis spp. was obtained with PVC (10 cm in diameter) from a traditional shrimp pond in Semarang vicinity. As a comparison, healthy looking Penaeus monodon was also obtained from the same pond. The occurrence of WSSV in Dendronereis spp. was determined with 1-step and nested PCR using primer for WSSV major envelope protein, VP 28. The viral load was counted with 1-step Real Time PCR. The WSSV was detected in Dendronereis spp. with 1-step and nested PCR. The point prevalence of WSSV infection in Dendronereis spp. is 90 %. The viral load ranged from 0 to 1.9 x 104 copy of DNA/µg total DNA. The viral load in Dendronereis is comparable with that of naturally infected and at carrier state P.monodon from the same pond. This is the first report of WSSV load in naturally infected Dendronereis spp.
Co-Authors Alfabetian Harjuno Condro Haditomo D esrina Diana Chilmawati Nestin E.W. Ningrum Ocky Karna Radjasa slamet budi prayitno