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All Journal Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Aquaculture Journal Journal of Aquaculture and Fish Health PENA AKUATIKA : JURNAL ILMIAH PERIKANAN DAN KELAUTAN
slamet budi prayitno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Social Sciences Veterinary

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Aktivitas Antibakteri Ekstrak Jeruju (Acanthus ilicifolius) terhadap Pertumbuhan Vibrio harveyi Secara in vitro (ANTIBACTERIAL ACTIVITY OF JERUJU (ANACTHUS ILICIFOLIUS) EXTRACTS ON THE GROWTH OF VIBRIO HARVEYI IN VITRO) Gina Saptiani; Slamet Budi Prayitno; Sutrisno Anggoro
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to study the potential of jeruju (Acanthus ilicifolius) extract as anantibacterial for alternative therapy and control of bacterial diseases in prawn nurseries. Ethanol extractionwas prepared from jeruju’s leaves, trunks, fruits, and flowers..Each extract was prepared at differentconcentrations (50 ppm, 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm, 800 ppm,900 ppm, and 1000 ppm, respectively) and further tested its antibacterial activity against Vibrio harveyiusing the agar disc diffusion method. The results showed that A. ilicifolius is a potential antibacterial,extract of the leaves seemed to be more effective in inhibit the growth of V. harveyi compared to other partsof the plant.
THE PERFORMANCE OF BI-FILTER ON THE PRODUCTION AND DISEASE INCIDENCE OF PRAWN (PENAEUS MONODON FAB.) Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 4, No 1 (2000): Volume 4, Number 1, 2000
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

It has been long known that the most potent factor that influences prawn production was unfavorable environment due to industrial, agriculture and habitat wastes as well as improper application of good husbandry management. This research was designed to study the effect of biological filter on the growth of tiger prawn, water qualilv, and disease incidence. 7`wo sets of experiments, namely field stddy and laboratory experiment were conducted . Field survey was carrted out to collect field data on actual husbandry practices, production, water and soil quality. Laboratory experiment was aimed at evaluating the efhctiveness of biologicalfilter and disease incidence. Juvenile prawn e (j 5 gr.) at a density of 15 animals/M2; 20 animals/M2, and 25 animals/M2 were used as experimental animals. The observed parameters were field productivity, growth, survival rate, water quolitv, bacterial counts, soil quality and disease incidence. Experiments were carried in 3 replicates. Field survey showed that prawn production decreased steephr at the third production cycle as low as 627.65 kg/Ha compared to the first cycle (1893. 28 kg/lla}. Most prawn were weak, and very vulnerable to diseases. Bacterial count was more than I0Jcfii/ml. Water quality rapidly declined as prawn grew especially during the second and third cycles. Laboratory experiment with biologicalfilter in contrast demonstrated better water quality and healthier prawn,. although water quality declined slightly along with both the age and density. The growth rate was better than during field trials; among three treatments there were signdicant dyferences; the survival rate was best at density of I 5 animals/M2 but, the best estimated production was reached by density of 20 animals/M2 producing 2645 kg/Ha/cycle without any problems for around 4 cycles. Disease incidence and parasites infestation was undetected in the tank during the experiment.
THE EFFECT OF DIFFERENT C:N AND C:P RATIO OF MEDIA ON THE CONTENT OF POLYHYDROXYBUTYRATE IN BIOFLOC INOCULATED WITH BACTERIUM Bacillus cereus Supono .; Johannes Hutabarat; Slamet Budi Prayitno; YS Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 2 (2013): VOLUME 16, NUMBER 2, YEAR 2013
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Biofloc technology  has  added values in aquaculture management, both in water quality  management and feeding  management.  As an optional feed, biofloc is capable to enhance growth due to high protein content.  Bacteria, main component biofloc, can produce polyhydroxybutyrate (PHB) as reserve of energy and growth accelerator for fish. The aim of the research were to study the effect of the different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc and to determine optimum media to produce high polyhydroxybutyrate content in biofloc. The experiment was arranged in factorial  with completely randomized design  in  three replications.  Treatments were C:N ratio of 15, 20, 25 and C:P ratio of 75, 100, and 125. The result showed that C:N ratio and C:P ratio of media and their interaction  affect the content of polyhydroxybutyrate in biofloc.  C:N ratio of 20 and C:P ratio of 125 resulted in most polyhydroxybutyrate (29.25±7.376 mg g-1 biofloc dry weight). Ratio of  C:N  of media gave linier and quadratic responses and C:P ratio of media gave linier one. Optimum polyhydroxybutyrate production was obtained at C:N ratio of 20.9 and C:P ratio of  125 resulting in 29.66 mg  g-1 biofloc dry weight (2,97%)
APPLICATION OF REPETITIVE SEQUENCE-BASED PCR ON THE RICHNESS OF VIBRIO ON THE TIGER SHRIMP (Penaeus monodon Fab.) S arjito; Nestin E.W. Ningrum; Ocky Karna Radjasa; Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A molecular-based study was conducted to estimate the richness of the Vibrio on  tiger shrimp (Penaeus monodon Fab.) from brackish water pond of Mororejo, Kendal. Tiger shrimps were collected from the extensive brackish water pond and a total of 22 isolates were obtained from hepatopancreas and telson of tiger shrimp. Based on the repetitive sequence-based polymerase chain reaction (rep-PCR), it was found that two groups of Vibrio. To investige the efectiveness of rep-PCR in estimating the richness of Vibrio on tiger shrimps, three isolates  (JTW 01, JTW 03 and JTW 06) were chosen for further investigation. On the basis of sequence analysis, the result showed  that the JTW 01, JTW 03 and JTW 06 were closely related to Vibrio sp. Absa7 clone 423.1, Vibrio splendidus and Vibrio splendidus, respectively.  The result proved that two assosiated of Vibrio on tiger shrimp were Vibrio sp. Absa7 clone 423.1 and Vibrio splendidus. Therefore  the present study highlights the effectiveness of rep-PCR in rapid grouping and estimating the richness of Vibrio on  tiger shrimp.
THE EFFECTIVENESS OF Acanthus Ilicifolius IN PROTECTING TIGER PRAWN (Penaeus monodon F.) FROM Vibrio harveyi INFECTION Gina Saptiani; Slamet Budi Prayitno; Sutrisno Anggoro
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 2 (2012): Volume 15, Number 2, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Sea holly (Achantus ilicifolius) is a mangrove plant that often used by coastal society as a traditional medicine.  It is potential to be developed as the sources of pharmaceutical products. This study aims at assessing the effectiveness of sea holly leaves as antibacterial agent and as an agent to enhance the durability of shrimp against Vibrio harveyi.  First, dried sea holly leaves were extracted with methanol, after that, it was fractionated with silica gel column method using solvent n-hexane, ethyl acetate, and n-butanol.  The treatments given to tiger prawn were crude extract, the fraction of ethyl acetate, and n-butanol, and it was given by immersion.   Next, the challenge test was conducted toward Vibrio harveyi.  As the result, the extract and the leaves of A. ilicifolius possess activities of inhibiting the growth of V. harveyi in vivo, reducing the prevalence of attacks and improving survival of prawn. In general, based on clinical symptoms and pathological anatomy, n-butanol fraction of the A.  ilicifolius leaves possess the best protection, along with ethyl acetate fraction and the crude.
DETERMINATION OF MERCURY’S BIOACCUMULATION FACTOR IN MILK FISH (Chanos chanos) OF SEMARANG MUNICIPALITY FISHPONDS USING NEUTRON ACTIVATION ANALYSIS Slamet Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 3, No 3 (2000): Volume 3 Number 3 Year 2000
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Determination of bioaccumulation factor of mercury (200Hg) in chanos chanos of Semarang municipality fishponds has been carried out by applying the neutron activation analysis (NAA) and gamma spectrometry analysis. The gamma spectrometry technique was employed to analyze the gamma radiation exposure from activated samples. The heavy metal 200 Hg can be identified in water and milk fish (Chanos chanos), along with natural radionuclides 40K in water. The concentration of Mercury in the water at the 3 villages was 0.950 10-6 ppm, 284 10-6, and 350 10-6 ppm respectively. Whilst mercury concentration in the milk fish was 186 10-3, 068 10-3, and 098 10-3 ppm for Mangunharjo, Mangkang, dan Karanganyar respectively. Bioaccumulation factor can be estimated from the ratio of these heavy metal element in the sample and the water. Result shows that the bioaccumulation factor for mercury at 3 villages (Mangunharjo, Mangkang dan Karanganyar) is 195.942, 238.961, 279.614 respectively.
THE GROWTH ANALYSIS OF Stichopus vastus (Echinodermata: Stichopodidae) IN KARIMUNJAWA WATERS Bambang Sulardiono; Slamet Budi Prayitno; Ign Boedi Hendrarto
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Stichopus vastus is one of sea cucumber resources utilize which has commercial economic value, locally known  as “gametes”. Because this species is continuously exploited, while the nature of life has not been known, one of them especially its growth.  Aspect of growth is an important parameter which is used as a basis for evaluating these resources, hence this resource is well managed and can be utilized in a sustainable manner. The study aims to: (1) estimates the growth characteristics mortality rate, recruitment and potential use of sea cucumber of  S.  vastus in Karimunjawa. The study is expected to give beneficial to the species of sea cucumber resource management strategy at  Karimunjawa waters, Jepara. The analysis of the growth parameters of S. vastus obtained values ​​of growth coeficient (K) of 0.55 year-1 and length infinity L ∞ value of 315.80 mm. Therefore the obtained values ​​of K tend to be close to zero value, it indicates the nature of growth tends to slow and with a relatively long life.  Life span of  S. vastus is 5.41 years, which can achieve body lengths of 283.06 to 296.91 mm, and the monthly growth rate ranged from 9.0 to 12.37 mm. The results of the analysis of mortality showed that S. vastus has value of total mortality (Z) of 0.98,  natural mortality (M) of 0.298, and catch mortality (F) of 0.682, with the rate of exploitation (E) of 0.6963. This suggests that the rate of exploitation is above the threshold standard set by the Government of 0.5 (BRKP, 2004). The analysis of the S. vastus the recruitment showed that the higest percentage recruit occurred in May-June is 17.16 - 18.33%. This is presumably due to a population increase of the spawning process in those months, although the value of the addition is not very significant.   It is suggested the need for regulations regarding: (a) the catchment season which is based on the tendency of the reproductive patterns of S. vastus, in Karimujawa, (b) restrictions on the size of the catch, (c) catch quotas, (d) the methods that are environmentally friendly catch, (e) permits the ship / boat used to catch sea cucumbers business. To support the success of regulation, it is necessary to strengthen community institutions through the management of sea cucumber resource-based society, with the Co-management approach.
EFFECT OF BIOACTIVE PROTEIN INGREDIENTS (MOTIVTM) ON TOTAL HEMOCYTE AND SURVIVAL RATE OF VANNAMEI SHRIMP, Litopenaeus vannamei Slamet Budi Prayitno; Bagus Rimbayu Ardie; Romi Novriadi; Vivi Endar Herawati; Seto Windarto
Indonesian Aquaculture Journal Vol 17, No 1 (2022): (June, 2022)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.17.1.2022.23-28

Abstract

One of the problematic factors in the cultivation of vannamei shrimp is the outbreak of bacteria and viruses. One way to prevent infection is by strengthening the shrimp’s immune system. MOTIVTM is a fermented corn protein concentrate that has been reported to possess probiotic properties that can positively increase the disease resistance of shrimp. Therefore, this study aimed to determine the effect of bioactive protein (MOTIVTM) on the total hemocyte count and shrimp survival rate. The study used an experimental method with a completely randomized design with five treatments and three replications: A (commercial feed), B (7.5% MOTIV add of 1.5% krill meal instead of 9% fish meal), C (7.5% MOTIVTM add of 1% krill meal to replaces of 2% poultry meal and 6.5% fish meal), D (7.5% MOTIVTM to replaces of 3.5% poultry meal and 4% fish meal), and E (7.5% MOTIVTM to replaces of 7.5% poultry flour). Feeding was done four times/day based on shrimp biomass and weekly feed counts based on daily shrimp survival. The vannamei shrimp used was one gram with a population density of 15 shrimps/aquarium with an aquarium volume of 100 liters.Total shrimp hemocytes, survival, and water quality were all measured. Treatment C had the highest total hemocyte, averaging 4.1 x 107 cells/mL, whereas treatment B had the lowest, averaging 1.4 x 107 cells/mL. During the trial, only treatment C supported 100% survival. 
APLIKASI TEKNIK PROBIOTIK TERHADAP KUALITAS AIR MEDIA BUDIDAYA IKAN LELE SANGKURIANG (Clarias gariepinus) DI DESA TAMBAKSARI, KECAMATAN ROWOSARI, KABUPATEN KENDAL Diana Rachmawati; Istiyanto Samidjan; Slamet Budi Prayitno
Pena Akuatika Jurnal Ilmiah Perikanan Dan Kelautan Vol 14, No 1 (2016): PENA AKUATIKA JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Fakultas Perikanan Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.001 KB) | DOI: 10.31941/penaakuatika.v14i1.501

Abstract

Application of probiotic technology is done to maintain water quality of aquaculture media of Sangkuriang catfish in Tambak Sari Village, Rowosari District, Kendal Regency. The aims of this study was to determine the effect of probiotic on water quality of Sangkuriang catfish cultivation medium in Tambak Sari Village. Sangkuriang catfish with age 15 days is material used in this study. Sangkuriang catfish kept for 45 days. The results showed there was a difference in the increase of body length and weight of Sangkuriang catfish on the culture medium with the application of probiotics and unprobiotics. media aquculture of applying probiotic techniques showed better and more feasible conditions for the cultivation of the Sangkuriang catfish.Key word : Sangkuriang catfish, aquaculture, probiotic
ECOLOGICAL INDICES OF MANGROVE GASTROPODS COMMUNITY IN NICKEL MINING IMPACTED AREA OF POMALAA, SOUTHEAST SULAWESI Purnama, Muhammad; Budi Prayitno, Slamet; Rudolf Muskananfola, Max; Suryanti, Suryanti
BIOTROPIA Vol. 31 No. 3 (2024): BIOTROPIA Vol. 31 No. 3 December 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.3.2267

Abstract

Article Highlights- Gastropods (Invertebrates: Mollusca) have the potential to be developed as biological indicators of the health of coastal aquatic environments- The development of gastropod communities has been proven to provide a significant ecological response in assessing environmental quality in the mangrove in relation to overburden waste input from nickel mining activities.- Overburden waste systemically degrades the ground floor (substrate) of the mangrove ecosystem as an area where the entire life cycle of the gastropod community is carried out.AbstractThis research aimed to determine the structure of gastropod community in the nickel mining impact area in the mangrove ecosystem of Tambea Village, Pomalaa District, Southeast Sulawesi Province. The scope of this research was gastropod community influenced by nickel mining activities, especially the impact of overburden waste input (reddish-orange colored sediment) toward the health status of the aquatic environment based on the ecological indices of the gastropod community in the mangrove ecosystem of Tambea Village. Two sampling methods were adopted in this research: (1) purposive sampling method to determine stations (locus) and (2) simple random sampling method to determine the distribution of substations or sampling points. Gastropod samples were taken using handpicking techniques. The structure of gastropod community in mangrove area affected by overburden waste showed low diversity index values (H’ = 0.81), low species richness (R = 1.75), moderate evenness (E = 0.50), and dominance of certain species (C = 0.54). The results of this research showed the massive impact of overburdened waste, which can systemically degrade the life of the typical fauna that make up the mangrove ecosystem. Three gastropods species were observed to live in the research location, namely Telescopium telescopium, Terebralia sulcata, and Terebralia palustris having low abundance (1-9 ind./m2) which can survive in environment exposed to overburden waste. Many of gastropod species were found dead, indicated by the finding of 2 shells of Ellobium aurisjudae. The input of overburdened waste may imply degradation system of the aquatic environment, especially in the mangrove ecosystem. This research offers outlooks of overburden waste on aquatic biota in mangrove ecosystems and other complimentary ecosystems. In the end, the condition of the gastropod community in a watered area becomes a basis of the health status of the water environment.
Co-Authors Aninditia Sabdaningsih Bagus Rimbayu Ardie Bambang Sulardiono Boedi Hendrarto Diana Rachmawati Fitriadi, Ren Gina Saptiani Gina Saptiani Istiyanto Samidjan Johannes Hutabarat Nestin E.W. Ningrum Ocky Karna Radjasa Purnama, Muhammad Romi Novriadi Rudolf Muskananfola, Max S arjito Sarjito - Seto Windarto Soedibya, Petrus Hary Tjahja Subagiyo Subagiyo Supono . Suryanti Sutrisno Anggoro VIVI ENDAR HERAWATI YS Darmanto