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All Journal HAYATI Journal of Biosciences Jurnal Sumberdaya Hayati Adi Widya : Jurnal Pengabdian Masyarakat Bioscientist : Jurnal Ilmiah Biologi Jurnal Nurse Jurnal Laboratorium Khatulistiwa Jurnal Medika Nusantara
Inayah, Mazidah Noer
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Community of Soil Actinobacteria in PTPN VI Oil Palm Plantation Jambi (Sumatra, Indonesia) Based on Amplicon Sequencing of 16S rRNA Gene Mazidah Noer Inayah; Yulin Lestari; Anja Meryandini
HAYATI Journal of Biosciences Vol. 29 No. 3 (2022): May 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.3.389-398

Abstract

In Sumatra, Indonesia, increased oil palm production encourages land expansion for oil palm plantations. And soil Actinobacteria have a potential role in agriculture and plantations ecosystems. The use of fertilizer and herbicide affects soil microbial diversity, including Actinobacteria. This research analyzed and investigated the community composition and diversity of Actinobacteria in soils of oil palm plantations in Jambi Sumatra. Amplicon-based analysis of the 16S rRNA gene (V3-V4 hypervariable region) was used to amplify actinobacterial full-length 16S sequences. The V3-V4 actinobacterial specific 16S rRNA gene sequencing was done using Next-Generation Sequencing. This study confirmed that actinobacterial specific 16S rRNA gene primer could amplify the actinobacterial 16S rRNA gene. Frankiales dominated the community composition of soilborne Actinobacteria. The diversity and community composition of soilborne Actinobacteria were not significantly affected by the interaction between fertilization and weed treatments. Furthermore, the use of NPK fertilizer significantly affected the abundance of Kineosporiales, whose abundance increased with the increasing concentration of NPK fertilizer. The interaction between fertilization and weeding treatments in the oil palm plantations has no impact on soil Actinobacteria's community composition and diversity.
Karakterisasi Xilanase dari Bakteri Xilanolitik XJ20 asal Tanah Hutan Taman Nasional Bukit Duabelas Jambi Indonesia INAYAH NOER MAZIDAH; LAKSMI AMBARSARI; ANJA MERYANDINI
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.25-30

Abstract

Xylanase is an extracellular enzymes that can be produced by microbes which capable to degrade xylan into xilo-oligosaccharides (XOS) and xylose. Application of xylanase is still not optimal due to the lack of availability of superior microbial cultures producing xylanase. The purpose of this study was to determine the characteristics of xylanase produced by bacteria isolated from Forest land Bukit Duabelas National Park, Jambi, Indonesia. Xylanase produced by bacterial isolate XJ20 was selected for further enzymatic activity and characteritics assays. Xylanolytic activity was measured by using the 3.5-dinitrosalicylic acid (DNS) assay. High activity of xylanase was recorded at three times of incubation, which were 6 (0.015 U/mL), 14 (0.012 U/mL) and 20 (0.007 U/mL). The first peak and the second peak reached the highest activity at a temperature of 70 °C, however, the first peak reached the highest activity at pH 4.0 and a second peak at pH 7.0. pH 4.0 xylanase were incubated at room temperature and 70 °C has a half time consecutive 9 hours and 6 hours, whereas at pH 7.0, has a half time consecutive 4 hours and 3 hours. Stability of enzyme better at pH 4.0 and at room temperature. 
Potensi Bakteri Tanah Penghasil Senyawa Antimikrob Untuk Menghambat Pertumbuhan Bakteri Escherichia Coli dan Staphylococcus Aureus Mazidah Noer Inayah; Suci Indah Budiarti
Jurnal Nurse Vol. 4 No. 1 (2021): Januari : Jurnal Nurse
Publisher : STIKES KESETIAKAWANAN SOSIAL INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/nurse.v4i1.234

Abstract

Antimicrobials are molecules that inhibit the growth of microbes. Some bacterial species, consisting of actinomycetes and fungi, are capable of producing antimicrobial substances. Streptomyces, commonly referred to as soil bacteria, are a group of actinomycetes that produce multiple antimicrobial agents. Other soil bacteria that may produce antibiotics include Pseudomonas, Bacillus, Nocardia, Kibdelosporangium, and Roseobacter. Thereby, soil is one of the potential sources that is capable of being used as a research sample for discovering and collecting antibiotic-producing bacteria, especially new types of antibiotics that weren't previously discovered. The latest research about antimicrobials (antibiotics) has been driven by the high number of cases of bacterial resistance. The study aims to investigate the potential of antimicrobial-producing bacteria from the soil to inhibit the pathogenic bacteria Staphylococcus aureus and Escherichia coli. The research procedure involves soil sampling, isolating the antimicrob-producing bacteria, describing the morphology of the bacterial colony and bacterial cells, purifying and regenerating the bacterial culture, and testing the activity of antimicrobial substances against E. coli and S. aureus. The study revealed four bacterial isolates: E1, E2, S1, and S2, that could possibly suppress the growth of S. aureus and E. coli. However, the antimicrobial activity of the four bacteria remained considerably smaller compared with 100 mg of chloramphenicol.
Gambaran Hasil Pemeriksaan Sifilis pada Ibu Hamil di Rumah Sakit Wijayakusuma Purwokerto Oksita Asri Widyayanti; Mazidah Noer Inayah; Esa Dhiandani; Nunuk Herowati
Jurnal Medika Nusantara Vol. 1 No. 1 (2023): Februari : Jurnal Medika Nusantara
Publisher : Stikes Kesdam IV/Diponegoro Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59680/medika.v1i1.1332

Abstract

Syphilis is a sexually transmitted disease caused by the bacteria Treponema pallidum. Syphilis is a resilient and systemic disease. Syphilis is passed on sexually by vaginal, anogenital, and orogenital contact. Non-sexual interactions, such as physical contact and blood transfusions, also remain possible. The disease can also spread vertically from mother to fetus (transplacental), resulting in congenital syphilis in the fetus. According to the Ministry of Health of the Republic Indonesia, there were 7055 new cases of syphilis in Indonesia during 2018. According to the latest data, 6% of pregnant mothers in Pekalongan possessed syphilis. Other studies found that 13% of pregnant mothers in Northern Halmahera were infected with T. pallidum or proved positive for syphilis. The number of cases of pregnant mothers suffering from syphilis encouraged the conduct of this study to figure out the features of pregnant mothers suffering from syphilis recognized at Wijayakusuma Hospital, Purwokerto, Banyumas. In this study, pregnant mothers were examined using the rapid test for syphilis. The findings revealed that 20 pregnant mothers were negative for T. pallidum or syphilis.
PENINGKATAN KOMPETENSI MENULIS BAGI MAHASISWA POLITEKNIK YAKPERMAS BANYUMAS MELALUI PELATIHAN PENULISAN ARTIKEL ILMIAH Inayah, Mazidah Noer; Budiarti, Suci Indah; Widyayanti, Oksita Asri; Nurcahyanti, Khusnul Khotimah Arum; Rahmansyah, Ilham; Alifah, Assha Luthfianie
Adi Widya : Jurnal Pengabdian Masyarakat Vol 9 No 2 (2025): Adi Widya: Jurnal Pengabdian Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/awpm.v9i2.13081

Abstract

Menulis artikel ilmiah seringkali menjadi tantangan tersendiri bagi mahasiswa. Beberapa kesulitan yang dihadapi mahasiswa dalam menulis diantaranya membuat parafrase kalimat, menyajikan dan menjelaskan data hasil penelitian, terbatasnya referensi, hingga rendahnya motivasi dalam menulis. Oleh karena itu, diperlukan kegiatan pelatihan penulisan artikel ilmiah bagi mahasiswa dengan tujuan memberikan pengetahuan dan pemahaman (edukasi) serta melatih keterampilan  menulis artikel ilmiah dengan baik agar menghasilkan publikasi ilmiah yang berkualitas serta bermanfaat. Kegiatan pelatihan penulisan artikel ilmiah bagi mahasiswa tingkat akhir di Politeknik Yakpermas Banyumas diselenggarakan dalam 2 hari. Hari pertama yaitu penyampaian materi tentang artikel ilmiah, kemudian dilanjutkan hari kedua yaitu asistensi penulisan artikel ilmiah. Pelatihan ini diikuti oleh 85 mahasiswa tingkat akhir yang berasal dari tiga program studi yaitu D-III Keperawatan, D-III Rekam Medis dan Informasi Kesehatan, dan D-IV Teknologi Laboratorium Medis. Sebanyak 5 orang peserta dengan artikel ilmiah terbaik diberikan apresiasi berupa penerbitan artikel pada Journal of Nursing and Health.
Isolasi dan Identifikasi Molekuler Bakteri Salmonella Asal Tempe Dage Berdasarkan Gen 16S rRNA Inayah, Mazidah Noer; Budiarti, Suci Indah; Wulan, Rahayu
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18200

Abstract

This study aimed to isolate Salmonella from empe dage, particularly products sold in traditional markets in the Banyumas region, Central Java, and to identify the isolates through molecular analysis of the 16S rRNA gene. Isolation of Salmonella from dage samples was conducted using culture-based methods on several types of selective media specific for Salmonella. Identification was performed through biochemical characterization using the API 20E system and molecular analysis based on PCR amplification of the 16S rRNA gene. The resulting 16S rRNA sequences were subsequently analyzed using the BLAST tool available on the NCBI platform, and phylogenetic relationships were reconstructed with MEGA X. The study successfully obtained one bacterial isolate (TD.1), exhibiting transparent colonies with black centers on SSA medium. Biochemical tests including catalase activity, sugar fermentation, H₂S production, and citrate utilization showed positive results. Further identification using the API 20E system confirmed that isolate TD.1 belonged to the genus Salmonella. Molecular analysis of the 16S rRNA gene identified isolate TD.1 as Salmonella enterica. In conclusion, isolate TD.1, recovered from tempe dage was identified as Salmonella enterica. The presence of Salmonella enterica in dage highlights a potential health risk and underscores the need for improved food hygiene in traditional markets.
Kandungan Formalin dan Jumlah Total Bakteri pada Produk Sosis di Pasar Tradisional Moga Kabupaten Pemalang Jawa Tengah Alimi, Ramadan Nur; Ambarwanty Mohtar, Cici Farhana; Inayah, Mazidah Noer
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2091

Abstract

One sort of frozen food that is made from processed meat is sausage. Formalin is one of preservative that is frequently abused, which can found in market-sold sausages. Health issues can arise from consuming foods containing formalin for an extended period of time. The filthy market environment, dust, and touch between customers' and sellers' hands can all lead to microbial contamination of food that sold in traditional markets. There has never been any prior research on the formalin content and bacterial contamination of sausage products sold in Pemalang Central Java's traditional markets.  Therefore, the purpose of this study was to figure out the amount of formalin and the quantity of bacteria contaminating sausage products that are sold at Moga traditional market in Pemalang, Central Java.  There were 6 samples of sausage were chosen randomly from several suppliers. A formalin test kit was used to qualitatively analyze the formalin, and the TPC method was employed for counting the bacteria. The result showed that formalin was absent from every sausage products. However, the TPC indicated that 4 of 6 sausages were contaminated with bacteria, S01, A02, S03, and S06, with counts of 1,2 × 10⁴ CFU/mL, 8.6 × 10⁴ CFU/mL, 8.7 × 10⁴ CFU/mL, and 3 × 10⁴ CFU/mL, respectively. Fortunately, the overall bacterial count remained below the acceptable level for human consumption.
Co-Authors Alifah, Assha Luthfianie Alimi, Ramadan Nur Ambarwanty Mohtar, Cici Farhana Anja Meryandini Budiarti, Suci Indah Epsi Marwati Esa Dhiandani Khusnul Khotimah Arum Nurcahyanti LAKSMI AMBARSARI Martha Intan Nagari Pratiwi Nunuk Herowati Oksita Asri Widyayanti Rahmansyah, Ilham Suci Indah Budiarti Widyayanti, Oksita Asri WULAN, RAHAYU YULIN LESTARI