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All Journal Indonesian Journal of Biotechnology Jurnal Biodjati
I Nyoman Pugeg Aryantha
School of Life Sciences and Technology, Institut Teknologi Bandung
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology

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Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum Sari, Indah Juwita; Wahyuni, Indria; Khastini, Rida Oktorida; Awaliyati, Ewi; Susilowati, Andriana; Utari, Enggar; Aryantha, I Nyoman Pugeg
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13191

Abstract

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.
Antibacterial activity of mycelial extract from a local fungus, Sclerotium rolfsii Zahra, Muhandinni; Aryantha, I Nyoman Pugeg; Sukrasno, Sukrasno; Suhardi, Veronica Sri Harjati
Indonesian Journal of Biotechnology Vol 30, No 3 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.108408

Abstract

Mycelium‐to‐sclerotium differentiation in fungi involves not only morphological but also biochemical changes throughout the process, which may contribute to their persistence and be a possible source of bioactive compounds. This study aims to evaluate the antibacterial activity and identify the bioactive compound in the local isolate Sclerotium rolfsii. Fungal culture was grown in media containing potato extract (20 g/L), dextrose (20 g/L), and peptone (5 g/L) for 27 days under static conditions at room temperature. Mycelium, sclerotium and filtrate were collected every three days and extracted with methanol, followed by evaporation and antibacterial screening. Significant activity was observed in day three of mycelial extract, which showed morphology of initial sclerotium formation (MIC 0.39 mg/mL) against B. subtilis and E. coli. An improved extraction method (sequential extraction) was employed for mycelial sample on the third day. N‐hexane and ethyl acetate extracts exhibited stronger activities (0.20 mg/mL). Ergosterol was identified after TLC‐bioautography, radial chromatography, and NMR elucidation analysis. S. rolfsii mycelium (third day‐sclerotial initiation) was found to contain ergosterol, demonstrating strong defense against bacteria, and possibly related to sclerotium‐differentiation metabolites. These findings may pave the way for more extensive studies of sclerotium differentiation as an interesting phenomenon of fungal development and bioactive compound origins.
Co-Authors Awaliyati, Ewi Fachry Abda El Rahman Indah Juwita Sari Rida Oktorida Khastini Suhardi, Veronica Sri Harjati Sukrasno Sukrasno Susilowati, Andriana Utari, Enggar Zahra, Muhandinni