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All Journal Indonesian Journal of Biotechnology Jurnal Biodjati 3BIO: Journal of Biological Science, Technology and Management
I Nyoman Pugeg Aryantha
School of Life Sciences and Technology, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Neuroscience

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Characterization of Plant Growth Promoting Rhizobacteria (PGPR) on Capcissum annum Sari, Indah Juwita; Wahyuni, Indria; Khastini, Rida Oktorida; Awaliyati, Ewi; Susilowati, Andriana; Utari, Enggar; Aryantha, I Nyoman Pugeg
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.13191

Abstract

Plant Growth Promoting Bacteria Rhizobacteria (PGPR) is one of the potential bacteria to enhance of Capsicum annuum through inhabitation the growth of pathogenic fungi. This study aimed to characterize PGPR in chili plants (Capsicum annuum). PGPR was isolated from the soil habitat of the red chili plant in Cilegon, Indonesia. Screening was then carried out with the dual culture method on Petri dishes and tested through in vivo method on the red chili plant. The selected bacteria were characterized morphologically, biochemically, and physiologically. The results revealed that there were 14 single isolates of bacteria from the roots of the red chili plants. The five single bacterial isolates, namely Azostobacter, Azospirillum, Pseudomonas, Serratia, and Beijerinckia have good potential as PGPR based on multiple culture screening by producing clear zones and positively effect the growth of chili plants.
Antibacterial activity of mycelial extract from a local fungus, Sclerotium rolfsii Zahra, Muhandinni; Aryantha, I Nyoman Pugeg; Sukrasno, Sukrasno; Suhardi, Veronica Sri Harjati
Indonesian Journal of Biotechnology Vol 30, No 3 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.108408

Abstract

Mycelium‐to‐sclerotium differentiation in fungi involves not only morphological but also biochemical changes throughout the process, which may contribute to their persistence and be a possible source of bioactive compounds. This study aims to evaluate the antibacterial activity and identify the bioactive compound in the local isolate Sclerotium rolfsii. Fungal culture was grown in media containing potato extract (20 g/L), dextrose (20 g/L), and peptone (5 g/L) for 27 days under static conditions at room temperature. Mycelium, sclerotium and filtrate were collected every three days and extracted with methanol, followed by evaporation and antibacterial screening. Significant activity was observed in day three of mycelial extract, which showed morphology of initial sclerotium formation (MIC 0.39 mg/mL) against B. subtilis and E. coli. An improved extraction method (sequential extraction) was employed for mycelial sample on the third day. N‐hexane and ethyl acetate extracts exhibited stronger activities (0.20 mg/mL). Ergosterol was identified after TLC‐bioautography, radial chromatography, and NMR elucidation analysis. S. rolfsii mycelium (third day‐sclerotial initiation) was found to contain ergosterol, demonstrating strong defense against bacteria, and possibly related to sclerotium‐differentiation metabolites. These findings may pave the way for more extensive studies of sclerotium differentiation as an interesting phenomenon of fungal development and bioactive compound origins.
Optimization of Ganoderma lucidum Mycelium Production for Biomaterial Production on Solid Medium with Bamboo and Empty Palm Oil Bunch Variation Dewi, Mustika; Susanto, Rakha Pradipa Bhagaskara; Aryantha, I Nyoman Pugeg
3BIO: Journal of Biological Science, Technology and Management Vol. 8 No. 2 (2026)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2026.8.2.5

Abstract

The chemical composition of sengon wood is ideal for the nutritional needs of fungi, particularly wood fungi. Fungi obtain their energy by degrading the lignocellulosic components (cellulose, hemicellulose, and lignin) found in wood. Although sengon is an excellent substrate, its availability is limited, and its price continues to rise. Potential alternative cultivation media include bamboo and oil palm, which are the natural habitats of Ganoderma lucidum. This study investigates the potential of bamboo sawdust and shredded empty palm oil fruit bunches (EFB) as alternative substrates for growing G.lucidum. Currently, G. lucidum is cultivated using a cereal-based medium with sengon sawdust waste. This study examines the growth of G. lucidum in media containing bamboo sawdust and EFB as an alternative media derived from organic waste. The experiment involves three stages: inoculating G. lucidum into media with varying concentrations of bamboo sawdust and EFB, adapting the fungus to complex media with different concentrations of these materials, and finally substituting sengon sawdust with bamboo sawdust or EFB in a control medium to observe growth parameters. The results showed that most media supplemented with bamboo sawdust and EFB, except for the 2% and 4% bamboo sawdust concentrations in the first stage, significantly increased growth (p < 0.05). However, in the second stage, only certain concentrations (25% bamboo sawdust and 50% EFB) achieved the same growth rate as the control. In the third stage, G. lucidum in media modified with bamboo showed significantly faster growth, although the loss of organic matter was not significantly different. Although bamboo and EFB did not increase the biomass production of G. lucidum mycelium both showed potential as alternatives. These findings indicate that these materials are suitable for use as a growth medium for G. lucidum.
Co-Authors Awaliyati, Ewi Fachry Abda El Rahman Indah Juwita Sari Mustika Dewi Rida Oktorida Khastini Suhardi, Veronica Sri Harjati Sukrasno Sukrasno Susanto, Rakha Pradipa Bhagaskara Susilowati, Andriana Utari, Enggar Zahra, Muhandinni