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Larvicide Effects of Serratia marcescens strain MBC1 Extract on Instar III Larvae of Aedes aegyti Lestari, Mutia Dinda; Nukmal, Nismah; Setyaningrum, Endah; Farisi, Salman; Arifiyanto, Achmad
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.219

Abstract

Bakteri merupakan kandidat yang berpotensi sebagai larvasida Aedes aegypti. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak Serratia marcecsens strain MBC1 terhadap kematian larva instar III Aedes aegypti. Penelitian ini merupakan penelitian eksperimental dengan dua macam bentuk pengujian, yaitu uji larvasida untuk menentukan mortalitas dan uji lanjut untuk melihat perkembangan larva setelah perlakuan larvasida. Larva instar III diberi perlakuan dengan konsentrasi 125, 250, 500, dan 1.000 ppm. Uji larvasida diamati selama 24-72 jam. Uji lanjut larva instar III  diberi perlakuan dengan konsentrasi 500 dan 1.000 ppm serta diamati sampai semua larva mati. Kontrol positif menggunakan Abate® 1% dan kontrol negatif menggunakan air sumur. Hasil analisis probit didapatkan bahwa nilai LC50 dan LC90 ekstrak S. marcescens adalah 66.426,02 dan 749.001,41ppm. Larva instar III mati pada 18 hari setelah perlakuan ekstrak S. marcescens strain MBC1. Hasil dari penelitian ini menunjukkan bahwa larvasida ekstrak S. marcescens mempengaruhi lamanya siklus hidup dan mati sebelum mencapai stadium dewasa.     Bacteria have potencial as larvicide of Aedes aegypti. The purpose of this study is to determine the effect of Serratia marcescens strain MBC1 against larval third instars of Aedes aegypti. This research was an experimental study with two kinds of testing. The larvicide test to determine the mortality of the larval and the continuous test to observe the development of the larval after treatment with the larvacide. The third instars of mosquito larval were given bacterial extract in four concentrations (125, 250, 500, and 1000 ppm), and observed mortality during 72 hours. The continuous larvicide test was observed at two concentrations (500 and 1,000 ppm). The observation is completed when all of the larvae died. The positive control used Abate® 1% and the negative control used natural water. The treatment was repeated 4 times. The results show that LC50 and LC90 of S. marcescens extract are 66,426.02 dan 749,001.41 ppm.  All of the larvae in the continuous test die after 18 days have been givenan extract of S. marcescens strain MBC1. The experiment shows that the larvicide of extract of S. marcescens influences the length of the life cycle and larvae of Ae. aegypti die before reaching the adult.    
Laju Pertumbuhan, Biomassa, dan Produksi Fikosianin Arthrospira platensis Perairan Pulau Jawa Pada Variasi Salinitas Lestari, Mutia Dinda; Sajidan; Pangastuti, Artini
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18387

Abstract

This study aims to identify and determine the optimal salinity that can produce specific growth rates, maximum dry biomass production, and the highest phycocyanin content and productivity in local strains of A. platensis in the waters of Java Island. This study was conducted using three strains of Arthrospira platensis originating from Jepara, Bogor, and Yogyakarta with salinity variations of 10, 20, and 30 ppt and three replicates using walne fertilizer culture media. The cultures were cultivated for eight days to calculate the growth rate and measure the biomass and phycocyanin concentration. The results of this study showed that the biomass of A. platensis in all strains was maximum at 30 ppt. The specific growth rate of A. platensis strains JPR and BGR was fastest at 30 ppt, and phycocyanin production was highest at 20 ppt. Meanwhile, the MRP strain showed a fast growth rate at 10 ppt and the highest phycocyanin production at 30 ppt. Based on the results of this study, it can be concluded that each strain has a different strategy in responding to salinity in terms of growth rate, biomass, and phycocyanin production.