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Karakterisasi Senyawa Flavonoid Ekstrak Polar Daun Gamal Kultivar Lampung Utara Dan Uji Aktivitasnya Terhadap Kutu Putih Kakao (Planococcus minor, Hemiptera: Pseudococcidae) Nukmal, Nismah; Pasutri, Agata Yelin; Pratami, Gina Dania
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.705 KB) | DOI: 10.14710/bioma.21.1.25-34

Abstract

Cocoa is one of the export commodities that have great prospects. The quality of cocoa in Indonesia are not satisfactory, this is caused by cocoa mealybug (Planococcus minor). Gamal (Gliricidia maculata) contains flavonoid compounds which can be used as bioinsecticides. The aims of research to determine the characteristic of flavonoid compounds in gamal leaf powder which is effective for kill of cocoa mealybug P. minor. Extraction and spectroscopy analysis (UV-Vis and FTIR) were done in the Integrated Laboratory of Technology Innovation Center (ILTIC) and the bioassay was done at Zoology Laboratory of Unila. The results shows that the crude water extract of gamal leaf powder North Lampung Cultivar (NLC) more effective killed lower than purified water extract. It was indicated by value of LC50.72hours the crude water extract than purified water extract (0.11%: 0.27%). The flavonoid compounds of gamal leaf powder NLC have the characteristics of blue fluorescence in UV light λ 254 and 366 nm. The flavonoid with the maximum peak of wavelength 310 could be grouped to flavonone with structural frame O-H, C=O carbonyl, aromatic C=C, and C-O.
Keberadaan Serangga Malam Berdasarkan Efek Warna Lampu Di Kebun Raya Liwa Faradila, Annisa; Nukmal, Nismah; Pratami, Gina Dania; Tugiyono, T
Bioma : Berkala Ilmiah Biologi Vol. 22, No 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.22.2.130-135

Abstract

Insects are invertebrate animals with diversity and have a high adaptability. Insects have properties that are very sensitive to light, especially night insects. Night insect species in the Liwa Botanical Garden (KRL) have not been identified. This research was conducted from October to November 2019 in the Liwa Botanical Garden, West Lampung Regency. The purpose of this study was to determine the effect of the color of the lights (red, yellow, blue and white) on the presence of night insects on KRL. This research was conducted using the Purposive Sampling method using red, yellow, blue and white light. The insects that were caught were identified in the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences Unila and analyzed descriptively based on morphological similarities with the book Borror et al., (1992). The data obtained were analyzed using the ANOVA test using Minitab 19. The results of this study showed that in the Araceae area of the Taman Liwa Botanical Garden the blue light traps caught a maximum of 457 night insects while the red light traps caught the night insects with the least number 19 tail. This study also showed that light intensity correlated with the number of night insects obtained.
Moth Variation using 3 Kinds of Pineapple, Banana, and Tapai as Bait in Liwa Botanical Garden Kinanti, Astrid Febi; Nukmal, Nismah; Pratami, Gania Dania; Kanedi, M.
Jurnal Natur Indonesia Vol 18, No 2 (2020): oktober
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.18.2.92-100

Abstract

Moths are insects that belong to the Lepidoptera Ordo. Moths are attracted to volatile compounds. The examples of volatile compounds include alcohol, urea, and acetic acid. Pineapple, banana and tapai are three types of bait that will be used in this study, because all three types of bait contain volatile compounds in the form of scents that strong enough to attract moths. Liwa botanical garden is a recreational and educational place, located in West Lampung district, which was only approved by the Regional Government in 2007. Because there is onlya view data on the presence of moths, so it needs to conduct the research to know the variation types of moths that exist in Liwa botanical garden, West Lampung. This research is a Quasy Experiment with 3 kinds of baits, there are pineapple, banana, and tapai as a treatment with 5 replications in the form of sampling time. The result obtained that in AraceaeLiwa Botanical Garden were obtained by six families, nine genera, and nine species of moth. Nine species of moths are Nemophora sp, Nematopogon sp, Brahmaeawallichii, Rhodogastriaamasis, Creatonosgungis, Sundasceliaepelys, Rheumaptera sp, Noctua sp, and Epicnopterix sp
Effectiveness of Ovitrap Against Aedes aegypti Mosquito In Kemiling Raya Sub-District Bandar Lampung City And The Vulnerability Of Its Larvals To Temephos Indriyani, Iin; Rosa, Emantis; Dania Pratami, Gina; Nukmal, Nismah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.205

Abstract

Demam Berdarah Dengue (DBD) adalah salah satu masalah kesehatan masyarakat, yang disebabkan oleh Virus Dengue. Di Indonesia kasus DBD masih tinggi, khususnya di Lampung, salah satu Kota dengan angka kasus DBD yang masih tinggi adalah Bandar Lampung. Dari berbagai hasil penelitian diketahui ovitrap memiliki fungsi monitoring serta pengendalian Aedes sp.  Upaya pengendalian vektor nyamuk Ae. aegypti banyak dilakukan baik secara alami ataupun dengan cara kimia, cara kimia yang paling umum digunakan adalah penggunaan temephos. Penggunaan insektisida kimia seperti temephos secara terus menerus dapat menyebabkan resistensi. Tujuan penelitian untuk mengetahui efektivitas ovitrap di dalam dan luar rumah, mengetahui ovitrap index, serta status kerentanan larva nyamuk Ae. aegypti terhadap temephos di Kelurahan Kemiling Raya Kota Bandar Lampung. Penelitian dilakukan bulan Februari-April 2021. Ovitrap diletakkan di 142 rumah di dalam dan luar rumah, hasil telur dihitung, dipelihara dan diuji kerentananya. Bahan yang digunakan larva instar III hasil pemeliharaan hingga F1. Penelitian dilakukan dengan 4 kali ulangan, menggunakan 25 larva pada tiap gelas dengan larutan temephos 0,02 mg/L dalam 250 mL air dibiarkan kontak uji selama 1 jam dan masa pemulihan selama 24 jam. Hasil yang di dapat 83,10% rumah terdapat telur pada ovitrap dan 16,90% tidak ada telur, jumlah telur pada ovitrap di luar rumah (57%) lebih banyak dari dalam rumah (43%) serta ovitrap index yang didapatkan adalah 72,5%, persentase kematian 99% dan dikategorikan rentan.     Dengue Hemorrhagic Fever is one of the public health problems, which is caused by the Dengue Virus. In Indonesia, dengue cases are still high, especially in Lampung, one of the cities with a high number of dengue cases is Bandar Lampung. From various research results, it is known thatovitrap has the function of monitoring and controlling Aedes sp. Efforts to control the mosquito vector of Ae. aegypti is mostly done either naturally or chemically, the most commonly used chemical method is the use of temephos. The continuous use of chemical insecticides such as temephos can cause resistance. The purpose of the study was to determine the effectiveness of ovitrapinside and outside the home, to determine the ovitrap index, and the susceptibility status of Ae. aegypti against temephos in Kemiling Raya Village, Bandar Lampung City. The study was conducted in February-April 2021. Ovitrapswere placed in 142 houses inside and outside the house, egg yields were counted, maintained, and tested for susceptibility. Materials used for larvae instar III reared up to F1. The study was carried out with 4 replications, using 25 larvae in each glass with a solution of temephos 0.02 mg/L in 250 mL of water and allowed to contact the test for 1 hour and the recovery period for 24 hours. The results obtained were 83.10% of the house contained eggs in the ovitrap and 16.90% had no eggs, the number of eggs in the ovitrap outside the home (57%) was more than inside the house (43%) and the ovitrap index obtained was 72, 5%, the percentage of deaths is 99% and is categorized as vulnerable.    
Larvicide Effects of Serratia marcescens strain MBC1 Extract on Instar III Larvae of Aedes aegyti Lestari, Mutia Dinda; Nukmal, Nismah; Setyaningrum, Endah; Farisi, Salman; Arifiyanto, Achmad
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.219

Abstract

Bakteri merupakan kandidat yang berpotensi sebagai larvasida Aedes aegypti. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak Serratia marcecsens strain MBC1 terhadap kematian larva instar III Aedes aegypti. Penelitian ini merupakan penelitian eksperimental dengan dua macam bentuk pengujian, yaitu uji larvasida untuk menentukan mortalitas dan uji lanjut untuk melihat perkembangan larva setelah perlakuan larvasida. Larva instar III diberi perlakuan dengan konsentrasi 125, 250, 500, dan 1.000 ppm. Uji larvasida diamati selama 24-72 jam. Uji lanjut larva instar III  diberi perlakuan dengan konsentrasi 500 dan 1.000 ppm serta diamati sampai semua larva mati. Kontrol positif menggunakan Abate® 1% dan kontrol negatif menggunakan air sumur. Hasil analisis probit didapatkan bahwa nilai LC50 dan LC90 ekstrak S. marcescens adalah 66.426,02 dan 749.001,41ppm. Larva instar III mati pada 18 hari setelah perlakuan ekstrak S. marcescens strain MBC1. Hasil dari penelitian ini menunjukkan bahwa larvasida ekstrak S. marcescens mempengaruhi lamanya siklus hidup dan mati sebelum mencapai stadium dewasa.     Bacteria have potencial as larvicide of Aedes aegypti. The purpose of this study is to determine the effect of Serratia marcescens strain MBC1 against larval third instars of Aedes aegypti. This research was an experimental study with two kinds of testing. The larvicide test to determine the mortality of the larval and the continuous test to observe the development of the larval after treatment with the larvacide. The third instars of mosquito larval were given bacterial extract in four concentrations (125, 250, 500, and 1000 ppm), and observed mortality during 72 hours. The continuous larvicide test was observed at two concentrations (500 and 1,000 ppm). The observation is completed when all of the larvae died. The positive control used Abate® 1% and the negative control used natural water. The treatment was repeated 4 times. The results show that LC50 and LC90 of S. marcescens extract are 66,426.02 dan 749,001.41 ppm.  All of the larvae in the continuous test die after 18 days have been givenan extract of S. marcescens strain MBC1. The experiment shows that the larvicide of extract of S. marcescens influences the length of the life cycle and larvae of Ae. aegypti die before reaching the adult.