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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology
Siti-Azizah, Mohd Nor
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education

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Effect of Sperm Extender and Dilution Ratio on The Sperm Motility, Fertility and Hatchability of Depik fish Rasbora Tawarensis (Pisces: Cyprinidae) Eggs Muchlisin, Zainal Abidin; Aldila, Dhea Febby; Sarah, Putri Inten; Eriani, Kartini; Hasri, Iwan; Fadli, Nur; Muhammadar, Abdullah Abbas; Siti-Azizah, Mohd Nor; Kocabas, Filiz Kutluyer
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 20, No 2 (2024): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.20.2.%p

Abstract

The artificial breeding method for depik fish Rasbora tawarensis was not available, and the extenders and its dilution ratio are the crucial factors in artificial breeding of fishes. Herein, we tested several common sperm extenders and its dilution ratio  in the artificial breeding for depik fish. Two  series of experiments were performed to find the best extender and its dilution ratio. In the first experiment, five types of extenders, namely: Ringer’s solution, physiological solution, coconut water, sugarcane water, and roomie water. The second experiment studied four dilution levels of sperm and extender (v/v), namely: 1: 20, 1: 30, 1: 40, and 1: 50. Every treatment was conducted in four replicates and experiments were conducted in series so that the best results in the first experiment were used in the second.  The first experiment showed that Ringer’s is the best extender for depik sperm, and the second experiment revealed that Ringer’s at dilution ratio 1: 40 gave higher sperm motility, fertilization and hatching rates. Hence,  the best formula for the dilution solution of depik sperm is Ringer’s at the dilution ratio of 1: 40, sperm: extender (v/v).
DNA Barcoding of Six Commercially Important Groupers (Epinephelidae) from Langsa, Aceh, Indonesia Fadli, Nur; Jumiati, Sri; Razi, Nanda Muhammad; Damora, Adrian; Muchlisin, Zainal A.; Dewiyanti, Irma; Ramadhaniaty, Mutia; Harnelly, Essy; Habib, Ahasan; Siti-Azizah, Mohd Nor
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.328-335

Abstract

Groupers are among the fish groups that are difficult to recognize due to their high morphological similarities. Therefore, molecular techniques, particularly DNA barcoding, are extensively utilized to differentiate this fish group. This study aimed to analyze and validate six grouper species belonging to the Epinephelidae family that were harvested from Langsa district waters in Aceh province, Indonesia, based on DNA barcode data. It was conducted from June to December 2021, with the fish specimens collected from fishers at fish landing sites and the fish market in Langsa City, Aceh province. A total of 22 grouper sequences belonging to six species were generated, namely Epinephelus coioides, E. bleekeri, E. malabaricus, E. erythrurus, E. sexfasciatus and Mycteroperca poecilonotus (formerly Epinephelus poecilonotus). Genetic distance within these species ranged from 0.10 to 0.73% (average: 0.40%). Notably, E. malabaricus and E. coioides exhibited the closest genetic kinship (4.07%), while E. sexfasciatus and M. poecilonotus displayed the greatest genetic distance (19.33%). This study provides the first DNA reference for grouper in Langsa district, Indonesia, with significant implications for future sustainable grouper management.
DNA Barcoding of Commercially Important Groupers (Epinephelidae) in Simeulue and Banyak Islands, Aceh, Indonesia Fadli, Nur; Razi, Nanda Muhammad; Damora, Adrian; Muchlisin, Zainal A.; Ramadhaniaty, Mutia; Rahayu, Sri Riska; Harnelly, Essy; Habib, Ahasan; Siti-Azizah, Mohd Nor
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.1080-1091

Abstract

The groupers are a group of demersal fish that live in tropical and subtropical areas and are mostly linked to coral reefs. The fish are highly valued in international markets and are subjected to overfishing in the wild. Accurate fish resource identification knowledge is essential for sustainable fisheries management. This research is aimed to generate a reference COI sequence library of grouper species caught in Simeulue and Banyak Islands, Aceh. The study was conducted from April-September 2021 at sixteen fish landing sites in Simeulue and Banyak Islands. In total, this study generated 70 COI sequences representing 20 grouper species. Epinephelus (54%) was the most prevalent grouper genus at the study site, followed by Cephalopholis (19%), Plectropomus (13%), Variola (12%), Hyporthodus (1%), and Anyperodon (1%). According to the IUCN classification, 17 species (85%) fall into the category of Least Concern, two (10%) fall into the category of Vulnerable, and one (5%) falls into the category of Data Deficient. The average genetic distance based on the Kimura-2-Parameter (K2P) between specimens was 0.51% at the species level and 8.34% at the genus level. Overall, this study has provided the COI sequence database of grouper for the Simeulue and Banyak Islands.
Co-Authors Adrian Damora Aldila, Dhea Febby Essy Harnelly Habib, Ahasan Irma Dewiyanti Iwan Hasri KARTINI ERIANI Kocabas, Filiz Kutluyer Muhammadar, Abdullah Abbas Nanda Muhammad Razi Nur Fadli Rahayu, Sri Riska Ramadhaniaty, Mutia Sarah, Putri Inten Sri Jumiati, Sri ZAINAL ABIDIN MUCHLISIN