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Utilization of Probiotic and Organic-Cn on Ruminal Ecosystem In Vitro Astuti, Wulansih Dwi; Ridwan, Roni; Tappa, Baharuddin
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

Improvement of nutrient intake was needed to increase cattle productivity. Probiotics and organic Cr, as feed additives, might have beneficially improve ruminant microbial ecosystem, and optimize digestion process by rumen microbes. The objective of this study was to examine the effects of combination between probiotic or mixed probiotcs and organic Cr on rumen fermentation activity. An in vitro study was held based on randomized block design with 5 treatments and 3 replications. The treatments were (A) Control ration, (B) Control ration + probiotic TSD 10, (C) Control ration + probiotic TSD 10 + organic Cr, (D) Control ration + mixed probiotics, and (E) Control ration + mixed probiotics + organic Cr. Organic Cr concentration was supplemented 2 ppm, whereas probiotics supplement contained 109 cfu/g. Supplementation of probiotic and organic Cr increased dry matter and organic matter digestibility but decreased total VFA and NH3 concentration. Treatment E (combination between mixed probiotics and organic Cr) gave the highest dry matter (52.45 %) and organic matter (51.96 %) digestibilities. It also tended to increase NH3 and total VFA production. Supplementation of single or mixed probiotics showed no difference for dry matter and organic matter digestibilities, and proportion of individual VFA. Mixed probiotics gave higher VFA and NH3 concentration compared to single probiotic. Probiotics supplementation resulted in a tendency for higher acetate proportion, while organic Cr supplementation resulted in a tendency of higher propionate proportion. Key Words: Probiotic, Organic Cr, Ruminal Microbes, VFA

Performa Pertumbuhan Kukang Jawa (Nycticebus javanicus E. Geoffroy,1812) yang Diberi Tambahan Biskuit dan Monkey Chow dalam Pakan Farida, Wartika Rosa; Astuti, Wulansih Dwi; Sari, Andri Permata
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2109

This study aims to determine the effect of the addition of biscuits and monkey chow in the feed to the growthperformance of java slow loris (Nycticebus javanicus E. Geoffroy, 1812). Research has been conducted in CaptivitySmall Mammals, Zoology Division, Research Center for Biology - LIPI for 52 days consisted of a 10 days period offeed adaptation and 42 days (6 weeks) period of data collection. The material used is six java slow loris aged 1 - 1,5years consisted of three males and three females. Alternative feed given during the study were banana (Musa sp.),papaya (Carica papaya), fuji apple (Malus domestica), pear (Pyrus amygdaliformis), guava (Psidium guajava),cucumber (Cucumis sativus), sweet potato (Ipomea batatas), boiled quail eggs, biscuits sprinkled with sugar, monkeychow, crickets, and mealworm. Parameters measured were feed intake, body weight gain, feed efficiency, and totaldigestible nutrients (TDN). The data were analyzed using analysis of variance, followed by Duncans multiplerange test. The results showed the average of dry matter intake in PII (45.89 g / head / day) was significantly higher(P <0.05) than that in PI (43.19 g / head / day) and P0 (42,17g / head / day. The average of body weight gain ofslow loris in PI> PII> P0, namely respectively 3.49 g / head / day, 3.41 g / head / day, and 3.15 g / head / day,while the feed efficiency in the treatment PI> P0> PII, respectively 8.20%, 7.47%, and 7.44%. The average oftotal digestible nutrients (TDN) of slow loris in PI (96.33%) was significantly higher (P <0.05) than that in PII(92.91%) and P0 (85.50%). The data of nutrient consumption can be used as a basis for the preparation of rationsand feed for slow loris in captivity.Key words: growth, feed efficiency, Nycticebus javanicus, captive breeding

The Addition of Potato Biscuit in the Feed of Sumatran Slow Loris (Nycticebus coucang Boddaert, 1785) and Javan Slow Loris (Nycticebus javanicus Geoffroy, 1812): The effects on Digestibility and Feed Efficiency Farida, Wartika Rosa; Sari, Andri Permata; Astuti, Wulansih Dwi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThis experiment aims to determine the effect of the addition of potato biscuit on digestibility and feed efficiency in Sumatran slow loris (Nycticebus coucang). Research has been conducted on Small Mammals Captivity Breeding of Zoology Division, Research Center for Biology - LIPI for 66 days consisting of a 10 days adaptation period of feed and 56 days (8 weeks) data collection period. The material used is four Sumatran slow lorises (N. coucang) and two Javan slow lorises (N. javanicus). Feed given during the study are banana, papaya, dragon fruit, guava, passion fruit, boiled sweet potato, boiled egg potato biscuits, crickets, and meal worm. Feed treatment to Sumatran slow loris consisting of feed control (T0) and T0 plus potato biscuits (T1), while Javan slow loris was only fed T1 as a comparison. Parameters measured were consumption, digestibility, and feed efficiency. The most palatable feed types for Sumatran slow loris and Javan slow loris are banana, cricket, and meal worm. Mean of feed intake at T0 and T1 treatment was 38.63 and 37.42 g / head / day, and that of Javan slow loris is 42.51 g / head / day. Mean of dry matter digestibility of Javan slow loris> T1> T0, namely 92.02%, 91.21%, and 88.95% respectively; whereas the highest average feed efficiency (EPP) is at 12.06% for Sumatran Slow loris and 9.10% in Javan slow loris. The average of total digestible nutrients (TDN) of Javan slow loris> T1> T0, namely 87.04%, 85.34%, and 83.54% respectively.Keywords: consumption, digestibility, feed utilization, Nycticebus coucang, Nycticebus javanicus

PERFORMA PERTUMBUHAN KUKANG JAWA (NYCTICEBUS JAVANICUS E. GEOFFROY,1812) YANG DIBERI TAMBAHAN BISKUIT DAN MONKEY CHOW DALAM PAKAN Farida, Wartika Rosa; Astuti, Wulansih Dwi; Sari, Andri Permata
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2109

This study aims to determine the effect of the addition of biscuits and monkey chow in the feed to the growthperformance of java slow loris (Nycticebus javanicus E. Geoffroy, 1812). Research has been conducted in CaptivitySmall Mammals, Zoology Division, Research Center for Biology - LIPI for 52 days consisted of a 10 days period offeed adaptation and 42 days (6 weeks) period of data collection. The material used is six java slow loris aged 1 - 1,5years consisted of three males and three females. Alternative feed given during the study were banana (Musa sp.),papaya (Carica papaya), fuji apple (Malus domestica), pear (Pyrus amygdaliformis), guava (Psidium guajava),cucumber (Cucumis sativus), sweet potato (Ipomea batatas), boiled quail eggs, biscuits sprinkled with sugar, monkeychow, crickets, and mealworm. Parameters measured were feed intake, body weight gain, feed efficiency, and totaldigestible nutrients (TDN). The data were analyzed using analysis of variance, followed by Duncan's multiplerange test. The results showed the average of dry matter intake in PII (45.89 g / head / day) was significantly higher(P <0.05) than that in PI (43.19 g / head / day) and P0 (42,17g / head / day. The average of body weight gain ofslow loris in PI> PII> P0, namely respectively 3.49 g / head / day, 3.41 g / head / day, and 3.15 g / head / day,while the feed efficiency in the treatment PI> P0> PII, respectively 8.20%, 7.47%, and 7.44%. The average oftotal digestible nutrients (TDN) of slow loris in PI (96.33%) was significantly higher (P <0.05) than that in PII(92.91%) and P0 (85.50%). The data of nutrient consumption can be used as a basis for the preparation of rationsand feed for slow loris in captivity.Key words: growth, feed efficiency, Nycticebus javanicus, captive breeding

THE ADDITION OF POTATO BISCUIT IN THE FEED OF SUMATRAN SLOW LORIS (NYCTICEBUS COUCANG BODDAERT, 1785) AND JAVAN SLOW LORIS (NYCTICEBUS JAVANICUS GEOFFROY, 1812): THE EFFECTS ON DIGESTIBILITY AND FEED EFFICIENCY Farida, Wartika Rosa; Sari, Andri Permata; Astuti, Wulansih Dwi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2884

ABSTRACTThis experiment aims to determine the effect of the addition of potato biscuit on digestibility and feed efficiency in Sumatran slow loris (Nycticebus coucang). Research has been conducted on Small Mammals Captivity Breeding of Zoology Division, Research Center for Biology - LIPI for 66 days consisting of a 10 days adaptation period of feed and 56 days (8 weeks) data collection period. The material used is four Sumatran slow lorises (N. coucang) and two Javan slow lorises (N. javanicus). Feed given during the study are banana, papaya, dragon fruit, guava, passion fruit, boiled sweet potato, boiled egg potato biscuits, crickets, and meal worm. Feed treatment to Sumatran slow loris consisting of feed control (T0) and T0 plus potato biscuits (T1), while Javan slow loris was only fed T1 as a comparison. Parameters measured were consumption, digestibility, and feed efficiency. The most palatable feed types for Sumatran slow loris and Javan slow loris are banana, cricket, and meal worm. Mean of feed intake at T0 and T1 treatment was 38.63 and 37.42 g / head / day, and that of Javan slow loris is 42.51 g / head / day. Mean of dry matter digestibility of Javan slow loris> T1> T0, namely 92.02%, 91.21%, and 88.95% respectively; whereas the highest average feed efficiency (EPP) is at 12.06% for Sumatran Slow loris and 9.10% in Javan slow loris. The average of total digestible nutrients (TDN) of Javan slow loris> T1> T0, namely 87.04%, 85.34%, and 83.54% respectively.Keywords: consumption, digestibility, feed utilization, Nycticebus coucang, Nycticebus javanicus

Utilization of Probiotic and Organic-Cn on Ruminal Ecosystem In Vitro Wulansih Dwi Astuti; Roni Ridwan; Baharuddin Tappa
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Improvement of nutrient intake was needed to increase cattle productivity. Probiotics and organic Cr, as feed additives, might have beneficially improve ruminant microbial ecosystem, and optimize digestion process by rumen microbes. The objective of this study was to examine the effects of combination between probiotic or mixed probiotcs and organic Cr on rumen fermentation activity. An in vitro study was held based on randomized block design with 5 treatments and 3 replications. The treatments were (A) Control ration, (B) Control ration + probiotic TSD 10, (C) Control ration + probiotic TSD 10 + organic Cr, (D) Control ration + mixed probiotics, and (E) Control ration + mixed probiotics + organic Cr. Organic Cr concentration was supplemented 2 ppm, whereas probiotics supplement contained 109 cfu/g. Supplementation of probiotic and organic Cr increased dry matter and organic matter digestibility but decreased total VFA and NH3 concentration. Treatment E (combination between mixed probiotics and organic Cr) gave the highest dry matter (52.45 %) and organic matter (51.96 %) digestibilities. It also tended to increase NH3 and total VFA production. Supplementation of single or mixed probiotics showed no difference for dry matter and organic matter digestibilities, and proportion of individual VFA. Mixed probiotics gave higher VFA and NH3 concentration compared to single probiotic. Probiotics supplementation resulted in a tendency for higher acetate proportion, while organic Cr supplementation resulted in a tendency of higher propionate proportion. Key Words: Probiotic, Organic Cr, Ruminal Microbes, VFA

PENGARUH SUBSTITUSI RUMPUT GAJAH DENGAN LIMBAH SERAI PADA PAKAN RUMINANSIA TINGGI HIJAUAN TERHADAPKECERNAAN DAN PRODUKSI METAN SECARA IN VITRO Rusli Fidriyanto; Gunawan Priadi; Yashanti Berlinda Paradisa; Wulansih Dwi Astuti; Roni Ridwan; Rohmatussolihat Rohmatussolihat; Ki Ageng Sarwono; Muh Whatman; Yantyati Widyastuti
Agric Vol. 33 No. 2 (2021)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2021.v33.i2.p103-114

Serai merupakan bahan baku untuk produksi minyak serai. Indonesia masuk dalam sepuluh negara terbesar produsen minyak serai di dunia. Industri pengolahan minyak serai akan menghasilkan limbah serai. Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi rumput gajah (Pennisetum purpureum) dengan limbah serai terhadap fermentasi rumen secara in vitro. Penelitian ini menggunakan rancangan acak kelompok dengan 5 perlakuan (100R: limbah serai 0%, 75R: limbah serai 25%, 50R: limbah serai 50%, 25R: limbah serai 75%, 0R: limbah serai 100%) dan 4 waktu pengambilan rumen yang berbeda sebagai kelompok. Hasil penelitian menunjukkan bahwa substitusi rumput dengan limbah serai tidak memberikan perbedaan yang signifikan terhadap kadar bahan kering, namun dapat menurunkan kadar protein dan meningkatkan serat kasar serta lemak kasar secara signifikan (P<0,05). Hasil analisis keceranaan in vitro terhadap aktivitas fermentasi rumen, menunjukkan bahwa substitusi serai tidak mempengaruhi pH, kecepatan produksi gas, asam butirat, asam valerat, asam isobutirat, dan asam isovalerat baik dari segi jumlah maupun proporsi. Substitusi rumput dengan limbah serai sebesar 25% (75R) tidak menghasilkan penurunan kadar bahan organik, produksi gas potensial dan produksi metan (jam ke-24) namun terjadi penurunan kadar protein dan meningkatkan lag time secara signifikan (P<0,05). Penggunaan serai sebesar 50% dapat menurunkan produksi gas metan secara signifikan (P<0,05). Peningkatan konsentrasi limbah serai yang digunakan menyebabkan terjadinya perubahan profil VFA parsial yang disebabkan oleh adanya perubahan komposisi asam asetat. Penurunan rasio asam asetat/ propionat (A/P) secara signifikan terjadi pada penggunaan serai sebesar 100%. Penggunaan limbah serai dapat mensubtitusi rumput gajah hingga 25% pada pakan tinggi hijauan tanpa terjadi penurunan pada produksi gas potensial, kecernaan dan total VFA.

Evaluation of Enzyme Activity and Nutrient Content of Fermented Coconut Meal as a Feed Using Indigenous Microorganisms Dwi Triani, Hera; Gusri Yanti; Wulansih Dwi Astuti; Sri Suharti; Yetti Marlida
ANIMAL PRODUCTION Vol. 27 No. 2 (2025)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2025.27.2.365

Coconut pulp has potential as animal feed but is limited in poultry diets due to its high fiber content and low protein levels. Fermentation using indigenous microorganisms offers a practical approach to improving its nutritional value. This study aimed to evaluate the nutritional quality of coconut meal using indigenous microorganisms (IMOs). The initial phase of the study involved producing IMOs, followed by measuring pH, total colony, and cellulase and mannanase enzyme activities. IMOs were applied to ferment coconut meal using a completely randomized design with four treatments (0, 7, 14, and 21 days) and four replications. Observed parameters included cellulase and mannanase enzyme activities, pH, crude fiber, crude protein, crude fat, and nitrogen-free extract. The characteristics of IMOs were analyzed descriptively, while enzyme activities and the nutritional content of coconut meal were analyzed using ANOVA and Duncan’s Multiple Range Test. The results showed that IMOs had a pH of 3.3, were dominated by lactic acid bacteria with a total colony of 5.4 × 10¹² CFU/mL, had cellulase activity of 2.1 U/mL, and had mannanase activity of 7.73 U/mL. Cellulase activity at 7, 14, and 21 days was significantly higher (P<0.01), while mannanase activity was highest at 21 days (P<0.01), with the lowest pH observed at 14 and 21 days. The lowest crude fiber and crude fat content, as well as the highest crude protein content (P<0.01), were observed at 14 days of fermentation. Fourteen days of fermentation were identified as the optimal duration for enhancing the nutritional quality of coconut pulp.

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