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All Journal Microbiology Indonesia Jurnal Ilmiah Ilmu-Ilmu Peternakan AGRIC
Yantyati Widyastuti
Research Centre for Biology-LIPI. Jl. Juanda 18, Bogor 16002, Indonesia.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Social Sciences

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Ecological and Taxonomical Perspective of Yeasts in Indonesia WELLYZAR SJAMSURIDZAL; ARIYANTI OETARI; ATIT KANTI; RASTI SARASWATI; CHIHARU NAKASHIMA; YANTYATI WIDYASTUTI; ANDO KATSUHIKO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8632.042 KB) | DOI: 10.5454/mi.4.2.3

Abstract

In the course of ecological and taxonomical study of yeasts in Indonesia, a total of 2147 yeast isolates from 315 samples in the year 2003, 2005, 2006, and 2008 had been obtained from eight locations e.g. Liwa (Sumatera), Cibinong (Java), Cibodas (Java), Kutai (Kalimantan), Enrekang (Sulawesi), Pucak (Sulawesi), Gili and Kuta (Lombok), and Kupang (Timor). Leaves, flowers, litters, soils, epiphytic soils, insects and insect's nests were collected for yeasts isolation. Our molecular identification based on D1/D2 region of nuclear large-subunit rDNA and the internal transcribed spacer (ITS) regions sequence data on 525 representative isolates revealed that 306 isolates belong to 48 described species (18 genera) and 209 strains belong to 19 undescribed species (19 genera), and 10 isolates were discarded because of contamination. Based on their substrates, litter had the highest yeasts genera (19) followed by soils (18), flowers (10), leaves (6), epiphytic soils (4), and insects and insect's nests (4). Genera found on soils were also common on litters. Yeasts genera found on flowers and epiphytic soils were common on leaves and litters. The genera Aureobasidium, Cryptococcus, Pseudozyma, Rhodotorula and Sporidiobolus were found in all substrates. Based on their locations, Kutai had the highest number of genera (15) followed by Cibodas (10), Cibinong (10), Enrekang (10), Kupang (10), Pucak (9), Liwa (7), and Lombok (7). The genus Cryptococcus was found in all locations. Our study shed a light to detection of many new taxa of yeasts, 41% of yeasts found in this study represented novel taxa.
Karakteristik Endapan Cairan Rumen Sapi asal Rumah Potong Hewan sebagai Feed Supplement Agus Budiansyah; Resmi Resmi; Nahrowi Nahrowi; Komang G Wiryawan; Maggy T Suhartono; Yantyati Widyastuti
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol. 14 No. 1 (2011): Mei 2011
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.854 KB) | DOI: 10.22437/jiiip.v0i0.829

Abstract

The aims of this experiment were to  identify and characterize of sediment product from cattle rumen liquor as a source of amino acids, minerals and vitamins. The sediment were obtained as pellets upon centrifugation of rumen liquor at 10,000 g for 10 minutes for collection of supernatant. The sediment were evaluated for amino acids, minerals and vitamins composition and chararacterized for the pH,  solubility of dry matter, specific density, bulk and compacted bulk densities and angle of response. Result of the experiment showed that sediment contained higher minerals:  Na, K and Fe compared with the commercial premix, but lower in B-vitamins and amino acids. The composition of mineral Na, K, and Fe from rumen liquor of local cattle was 13.47%, 7.73 % and 14.52 %,  while Na, K, and Fe from  rumen liquor of  imported cattle was 18.40%, 10.25%, and 14.52% respectively. The sediment had pH range from 10.01-10.03, the dry matter solubility  was 35.5% up to 39.1%. The sediment from imported cattle had higher specific density, bulk and compacted bulk densities and angle of response than that of local cattle. It is concluded that sediment from cattle rumen liquor contained high Na, K and Fe, low amino acids and B-vitamin, high pH and low solubility.
PENGARUH SUBSTITUSI RUMPUT GAJAH DENGAN LIMBAH SERAI PADA PAKAN RUMINANSIA TINGGI HIJAUAN TERHADAPKECERNAAN DAN PRODUKSI METAN SECARA IN VITRO Rusli Fidriyanto; Gunawan Priadi; Yashanti Berlinda Paradisa; Wulansih Dwi Astuti; Roni Ridwan; Rohmatussolihat Rohmatussolihat; Ki Ageng Sarwono; Muh Whatman; Yantyati Widyastuti
Agric Vol. 33 No. 2 (2021)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2021.v33.i2.p103-114

Abstract

Serai merupakan bahan baku untuk produksi minyak serai. Indonesia masuk dalam sepuluh negara terbesar produsen minyak serai di dunia. Industri pengolahan minyak serai akan menghasilkan limbah serai. Penelitian ini bertujuan untuk mengetahui pengaruh subtitusi rumput gajah (Pennisetum purpureum) dengan limbah serai terhadap fermentasi rumen secara in vitro. Penelitian ini menggunakan rancangan acak kelompok dengan 5 perlakuan (100R: limbah serai 0%, 75R: limbah serai 25%, 50R: limbah serai 50%, 25R: limbah serai 75%, 0R: limbah serai 100%) dan 4 waktu pengambilan rumen yang berbeda sebagai kelompok. Hasil penelitian menunjukkan bahwa substitusi rumput dengan limbah serai tidak memberikan perbedaan yang signifikan terhadap kadar bahan kering, namun dapat menurunkan kadar protein dan meningkatkan serat kasar serta lemak kasar secara signifikan (P<0,05). Hasil analisis keceranaan in vitro terhadap aktivitas fermentasi rumen, menunjukkan bahwa substitusi serai tidak mempengaruhi pH, kecepatan produksi gas, asam butirat, asam valerat, asam isobutirat, dan asam isovalerat baik dari segi jumlah maupun proporsi. Substitusi rumput dengan limbah serai sebesar 25% (75R) tidak menghasilkan penurunan kadar bahan organik, produksi gas potensial dan produksi metan (jam ke-24) namun terjadi penurunan kadar protein dan meningkatkan lag time secara signifikan (P<0,05). Penggunaan serai sebesar 50% dapat menurunkan produksi gas metan secara signifikan (P<0,05). Peningkatan konsentrasi limbah serai yang digunakan menyebabkan terjadinya perubahan profil VFA parsial yang disebabkan oleh adanya perubahan komposisi asam asetat. Penurunan rasio asam asetat/ propionat (A/P) secara signifikan terjadi pada penggunaan serai sebesar 100%. Penggunaan limbah serai dapat mensubtitusi rumput gajah hingga 25% pada pakan tinggi hijauan tanpa terjadi penurunan pada produksi gas potensial, kecernaan dan total VFA.
Co-Authors A Budiansyah ANDO KATSUHIKO Ariyanti Oetari Atit Kanti CHIHARU NAKASHIMA Gunawan Priadi Ki Ageng Sarwono Komang G Wiryawan Maggy T Suhartono Muh Whatman Nahrowi Nahrowi RASTI SARASWATI Resmi Resmi Rohmatussolihat Rohmatussolihat Roni Ridwan Rusli Fidriyanto Wellyzar Sjamsuridzal Wulansih Dwi Astuti Yashanti Berlinda Paradisa