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DISTRIBUSI DAN KELIMPAHAN SEMAIAN Rhizophora PADA ZONA INTERTIDAL DI EKOSISTEM MANGROVE DESA JAGO-JAGO KABUPATEN TAPANULI TENGAH Reynhard '; Khairijon '; Mayta Novaliza Isda
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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This research was conducted from April until May 2013, and aimed to identify and analyze the distribution and abundance of Rhizophora seedlings in the intertidal zone of mangrove ecosystem in Jago-jago Village. The observed parameters were distribution, abundance, and water and soil quality. The observation of seedling sample at front, middle, and back of the intertidal zone were systematically conducted using a transect method, where each transect consisted of four plots (4m x 4m in size). The result found two Rhizophora species, namely R. apiculata and R. mucronata that have grown in environments with ideal conditions of temperature, salinity, and pH. The highest abundance of Rhizophora seedlings (11406,25 ind/ha) was found in the middle of intertidal zone. The distribution of Rhizophora seedlings at Jago Jago Village according to morisita index is uniform (Ip< 0).

PERBANYAKAN ANGGREK Grammatophyllum scriptum (Lindl.) BL. MELALUI INDUKSI TUNAS SECARA IN VITRO DENGAN PENAMBAHAN BAP dan NAA Angriawan Markal; Mayta Novaliza Isda; Siti Fatonah
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 2, No 1 (2015): Wisuda Februari 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Grammatophyllum scriptum (Lindl.) BL. is one of orchid species that pottentially to be extinct in the nature. In order to maintain the existence of this orchid, a propagation is needed, such as using in vitro culture techniques. This study aimed to determine the influence and the best concentration of BAP in single treatment, as well as the combination with NAA in inducing in vitro bud explant of G. scriptum. This study used a completely randomized design (CRD) with various concentrations of BAP only or in combination with NAA. The results of this study showed that the treatment of 1 mg /l BAP gave the best results with 100% orchid bud formation, 13.67 of formation of buds after planting day, with number of bud and and leave were 3,33 and 5,33, respectively. While the best combination of BAP and NAA for shoot growth was 1 mg / l BAP + 0.5 mg / l NAA, with the formation of shoot was 13,33 after planting day, 2.33 buds, and 5.67 leaves.

Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 76, No 2: Desember 2008
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet. Hasil analisis BlastX dari sekuen klon cDNA menunjukkan bahwa gen berukuran ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.

Inovasi teknologi hidroponik melalui pemanfaatan barang bekas dan nutrisi buatan sendiri sebagai usaha peningkatan pendapatan petani di Desa Kualu Kecamatan Tambang Kabupaten Kampar Fitmawati Fitmawati; Mayta Novaliza Isda; Isnaini Isnaini; Nery Sofiyanti; Rodesia Mustika Roza
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.499-505

The hydroponic technology of the wick system is one of the innovations in vegetable cultivation without soil with the innovation of utilizing used goods and household waste such as styrofoam and used cans as a container for planting. The purpose of this activity is to reduce waste in the community, improve the family's economic capacity, and people's interest in consuming clean and hygienic vegetables in Kualu Village, Tambang District, Kampar Regency. This hydroponic technique is made of relatively inexpensive and affordable materials so that the limitations of people's financial capabilities can be overcome. This system is a hydroponic system that uses axes to absorb organic nutrients. To minimize the cost of purchasing nutrients, it can be overcome by innovating nutrient solutions from household waste into liquid fertilizer. The implementation of community service uses counselling, discussion and making hydroponic technology. The community is very enthusiastic in joining this program, especially housewives who want to know the application of Wick's hydroponic technology so that they can play a large role in meeting food needs and increasing people's income.

PEMANFAATAN MULSA ORGANIK Imperata cylindrica (L.), Mucuna bracteata DC. DAN KOMPOS PELEPAH KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP PENGHAMBATAN PERKECAMBAHAN DAN PERTUMBUHAN GULMA Mikania micrantha H.B.K. Siti Fatonah; Herman Herman; Sari Malinda; Mayta Novaliza Isda
DINAMIKA PERTANIAN Vol. 30 No. 3 (2015): Jurnal Dinamika Pertanian Edisi Desember 2015
Publisher : UIR Press

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The aim of this study was to determine effect of the thickness and type of organic mulch (Imperata cylindrica, Mucuna bracteata and compost of palm oil midrib) on germination and growth of Mikania micrantha weeds in polybag at garden. The experiment was designed in Randomized Block Design, with different treatment of three types of mulch, alone or in combination at 2.5 and 5 cm thickness, which contains 11 treatments. Each treatment was repeated seven replications. The results showed that mulch of I. cylindrica, M. bracteata, palm oil compost and their combinations inhibited the germination and growth of M. micrantha weeds. Almost all treatments mulch inhibited germination and growth of M. micrantha to decrease to 100 percent, except for M. bracteata mulch treatment with the reduction reached 97 percent.

Feasibility Test of Papaya Microgreens (Carica papaya L. var. Callina) As Functional Foods sujarwati, Sujarwati; Mayta Novaliza Isda; Retno Ayu Wulandari; Anisa Sholihatul Muawanah
JURNAL SAINS NATURAL Vol. 15 No. 1 (2025): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v15i1.729

Papaya seeds have the potential to be developed as microgreens. Microgreens are classified as functional foods because they contain at least ten times more phytochemicals and vitamins than mature plants of the same species. This study aims to analyze the feasibility of Callina papaya microgreens as functional food based on proximate analysis, antioxidant activity, and antioxidant compound content. Proximate analysis was conducted by testing the moisture content (thermogravimetric), ash (dry soaking), protein (Kjeldahl method), fat (soxhlet method), and carbohydrate (by difference). The antioxidant activity test used the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. Analysis of antioxidant compounds included testing vitamin C levels (spectrophotometry) and chlorophyll levels (portable chlorophyll meter). Flavonoid, alkaloid, and tannin compounds were tested qualitatively. Proximate analysis of papaya microgreens on dry weight contained 57.35% water, 0.48% ash, 3.46% carbohydrate, 13.45% fat, and 25.28% protein. Papaya microgreens in wet weight percent contained 94.35% water, 0.03% ash, 3.43% carbohydrate, 0.76% fat, and 1.43% protein. Papaya microgreens have very strong antioxidant activity with an IC50 value of 2.058 ppm. Antioxidant compounds in papaya microgreens are vitamin C (1.152%), chlorophyll (32.56 μmol/m²), flavonoids, and alkaloids, but tannins are not present. Based on the results of proximate analysis, antioxidant activity test, and antioxidant compounds, it can be concluded that papaya microgreens are feasible as functional food because it meet the requirements of functional foods, which contain physiologically active compounds and can provide health benefits.

Kloning dan karakterisasi gen penyandi inhibitor proteinase dari kulit buah kakao Cloning and characterization of gene encoding proteinase inhibitor of cacao pod wall Mayta Novaliza ISDA; Musliar KASIM; . MANSYURDIN; Tetty CHAIDAMSARI; Djoko SANTOSO
Menara Perkebunan Vol. 76 No. 2: 76 (2), 2008
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v76i2.84

Summary Attempts to increase cocoa production in Indonesia have been hinderred by attack of CPB (Conopomorpha cramerella). There has been no effective measures to control this pest leading to development of cacao planting materials which resistant to the pod borer. One of genes functioning in plant defense system against insect pests such as catepilar is Proteinase Inhibitor (PIN). This research aimed to isolate and characterize TcPIN gene of cacao pod wall. A clone of TcPIN was isolated with RT-PCR technique using total RNA of cacao pod wall and DNA primer designed based on the sequence Trypsin Inhibitor of cocoa bean accessible online. BlastX analysis of the sequence of the cDNA clone demonstrated that the ± 600 bp gene cloned with pGEM-T was PIN gene as indicated by highly homologous to Trypsin Inhibitor of Theobroma microcarpum resulted in 248 Score bits and E value 1 e-64. Two sequence alligment with the putative 21 kDa PIN of cacao seed indicated a moderately high homology. Contrasting these two sequences however found some non identical amino acids implying some variations. Ringkasan Usaha peningkatan produksi kakao di Indonesia terkendala antara lain oleh adanya serangan hama PBK (Conopomorpha cramerella). Untuk menanggulangi serangan PBK tersebut perlu adanya satu cara pengendalian yang efektif dan efisien, sehingga dapat mendorong usaha pengembangan bahan tanam yang tahan PBK. Salah satu gen membawa sifat ketahanan tanaman terhadap hama ulat adalah Proteinase Inhibitor (PIN). Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi gen TcPIN dari kulit buah kakao. Klon cDNA TcPIN diisolasi dari kulit buah kakao dengan teknik RT-PCR meng-gunakan RNA total kulit buah kakao dan primer DNA yang dirancang atas dasar sekuen Inhibitor Tripsin biji kakao yang diakses lewat internet. Hasil analisis BlastX dari sekuen klon cDNA menunjukkan bahwa gen berukuran ± 600 pb yang telah diklon dengan pGEM-T tersebut adalah PIN karena memiliki homologi yang tinggi terhadap 21 kDa trypsin inhibitor dari Theobroma microcarpum yang meng-hasilkan Skor 248 bits dengan Nilai E 1e-64. Penjajaran dua sekuen dengan PIN putatif 21 kDa yang berasal dari biji kakao menunjuk-kan tingkat homologi yang tinggi, dengan perbedaan nyata sehingga dapat terlihat bahwa keduanya tidak identik.

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