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THE DNA SEQUENCE ENCODING GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPC) ENZYME ON TUNTUN ANGIN PLANT (ELAEOCARPUS FLORIBUNDUS BI) Dewi Indriyani Roslim; Hestia Hairima; Herman Herman; Wahyu Lestari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Glyceraldehyde 3-phosphate dehydrogenase (GapC) is an enzyme involved in glycolysis. The expression of this gene tends to abundant in eukaryotic cells, so this gene is frequently used as an internal control in gene expression analysis. This research aims to isolate the DNA sequence of the GapC gene from tuntun angin (Elaeocarpus floribundus BI). Methods included a collection of the leaves from Kajuik Lake, Riau Province then the DNA extraction, electrophoresis, amplification of partial DNA sequence of GapC gene, cloning and sequencing. The DNA sequence was analyzed using the BLASTn program and MEGA6 software. The GapC sequence obtained in this study was 933 bp in size, consisting of four introns and five exons, and encoding 137 deduced amino acids. The BLASTn analysis showed that the sequence has 89.84%-90.16% similarity to other species of Cunoniaceae family, such as species from the genus of Spiraeanthemum and Codia. The parsial sequence of E. floribundus GapC gene was more resemble the one of Spiraeanthemum than Codia genus. The GapC sequence obtained in this study was the first reported from the Elaeocarpaceae family. This sequence has the opportunity to serve as an internal control after validation.

ANALISA CITRA HIPERSPEKTRAL DAUN DARI TANAMAN KELAPA SAWIT YANG MENGALAMI KEKURANGAN AIR MENGGUNAKAN PROGRAM MATLAB JR Lessy Eka Putri; Minarni Minarni; Feri Candra; Herman Herman
Komunikasi Fisika Indonesia Vol 16, No 2 (2019)
Publisher : Universitas Riau

The hyperspectral imaging method has been widely and intensively used in agriculture to find out various problems that occur in plants. Image processing is very important step in an imaging method. This research aims to create Matlab based program to be used to analyze the leaf image of oil palm plants that has experienced water deficiency. Reflectance intensity values were extracted from the process. The hyperspectral imaging system consisted of a 650 nm diode laser, a spectrograph, monochrome CMOS camera, and Matlab image processing program. The samplesused were 8 month old Tenera variety of oil palm seedlings which were treated to simulate water deficiency in the form of variations in the volume of water, namely 0 mL (without watering), 1000 mL, 2000 mL, and 3000 mL (normal), 3 duplicates for each volume. The samples were given water volume of 1000 mL and 2000 mL for every 7 days in 21 days, while the sampleswith 3000 mL of water were watered every day. Image recording was done on the 21st day for detached leaves at the the bottom part.The results showed that the Matlab program was able to separate each row from 15 images, each of which had a pixel size of 1280 × 1024 and merge each of the same lines into 1024 images with a pixel size of 1280 × 15. The reflectance intensity values were then obtained. The results showed that higher levels of water deficiency in plants produce increasing reflectance intensity values.

APLIKASI PROGRAM MATLAB UNTUK ANALISA CITRA HYPERSPECTRAL PADA AKAR DARI TANAMAN KELAPA SAWIT YANG MENGALAMI KEKURANGAN AIR Mailestari Wina Yance; Minarni Minarni; Feri Candra; Herman Herman
Komunikasi Fisika Indonesia Vol 16, No 2 (2019)
Publisher : Universitas Riau

Hyperspectral images are three dimensional images which have two dimension spatial information and one dimension spectral information. Hyperspectral image processing using Matlab program is preferable because it is more adaptive for many analysis purposes. This research was aimed to construct Matlab to process and analyze the hyperspectral images of the roots of oil palm plants that have experienced water deficiency. The program was designed and constructed using a GUI . The use of a GUI aims to combine each pixel of the same line from each sample to produce a new image. The samples were roots of oil palm plants that experienced simulated water deficiency by giving different water volumes of 0 mL, 1000 mL, 2000 mL and 3000 mL (normal). The optical method used in this study is a hyperspectral imaging method which has 650 nm diode laser as the light source , spectrograph Specim Imspector V10 , and a monochrome CMOS as a detector. Reflectance intensity versus wavelength was extracted from each images and analyzed. The results showed that the Matlab GUI program that had been constructed was able to produce 1024 new images that had a pixel size of 15× 1280 from each sample. The results also show that the reflectance intensity values are higher at higher water deficiency of the oil palm roots.

Penguatan Iptek dan kearifan lokal dalam pengelolaan perikanan di Desa Pangkalan Jambi Kecamatan Bukit Batu Kabupaten Bengkalis Dadang Mashur; Ridwan Manda Putra; Herman Herman; Mayarni Mayarni; Mimin Sundari Nasution; Eka Hariyani; Musadad Musadad; Risky Arya Putri
Unri Conference Series: Community Engagement Vol 1 (2019): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.1.290-296

The coastal area has many potential resources whose utilization is not optimal. One potential that must be developed is the potential of human resources, with ability and creativity, humans can create and develop everything that is initially not considered and has a low selling value into something that has high economic value. The strengthening of science and technology aims to motivate rural communities, especially fishermen, to develop creativity so that they can increase the income and welfare of fishing communities. The science and technology strengthening activity was carried out in Pangkalan Jambi Village with two models, namely the application of the Center for Environment and Society Model, namely utilizing the productive use of social capital and environmentally friendly management of fisheries resources. The second application is the Entrepreneurship Capacity Building (ECB) model related to the ability of the entrepreneurial community. Science and technology strengthening activities and local wisdom in the form of fostering fishing communities in the management of fishery products such as chips, nuggets, meatballs, oil, as medicines and fish jelly as cosmetics. Then institutional development and assistance for marketing. So far, fishermen are only able to sell their catches directly without technology-based processing. After the dedication, there were three programs carried out including the formation of individual skills in fish processing, group formation, and marketing management assistance.

Pemberdayaan kelompok masyarakat Kecamatan Tampan Kota Pekanbaru dalam program inovasi teknologi pengering berbasis biomassa yang ramah lingkungan untuk meningkatan kualitas dan kuantitas produk makanan berbahan baku ubi Juandi Muhammad; Herman Herman; Gimin Gimin; Joko Risanto; Syahril Syahril
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.541-549

The potential of the agricultural sector in terms of cassava in Tampan District, Pekanbaru City with a total potential of 11,210 tonnes of cassava (based on BPS data, 2019). This enormous potential has led to the growth of SMEs for community groups to take advantage of these opportunities in the business of producing cassava-based food processing. Furthermore, this potential is an opportunity for local governments to create a market or regional superior product as well as an attraction for citizens from outside the region to come to Tampan District because it can form an agro-industry in the field of cassava chips. The attraction for visitors to stop by at Tampan District is also supported by geographical aspects, transportation that is easily accessible to everyone. Through this community service activity, the fostered village scheme is very appropriate to be applied in Tampan District because it can guide UKM groups so that at the end of the service activities of this guided village scheme will form superior regional products that are competitive through the application of environmentally friendly biomass energy-based drying technology, and the application of IT-based management system so that at the end of the activity it will produce an Agro-Industry target village with regional superior products that are competitive.

Analisis Empat Sekuen Barkode DNA Pada Pandan (Benstonea sp.) Asal Danau Kajuik, Riau Dewi Indriyani Roslim; Intan Sari Nuraini; Siti Nurhayati; Ciska Vivian Sianturi; At-Thahirah At-Thahirah; Herman Herman
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21697

Barkode DNA merupakan sekuen DNA berukuran pendek yang digunakan untuk identifikasi organisme secara molekuler. Penelitian bertujuan menganalisis empat barkode DNA pada tumbuhan pandan (Benstonea sp.) asal Danau Kajuik, Riau. Metode meliputi isolasi DNA, PCR, elektroforesis, purifikasi, sekuesing, serta analisis bioinformatika. Pada penelitian ini telah diperoleh sekuen DNA untuk atpB-rbcL IGS, trnV-ndhC IGS, ndhF-rpl32 IGS, dan trnQ-5’rps16 IGS sepanjang 812 pb, 924 pb, 952 pb, dan 886 pb, secara berturut-turut. Aksesi yang muncul paling atas pada analisis BLASTn pada keempat sekuen tersebut tidak ada yang memiliki kemiripan 100% dengan Benstonea sp. asal Danau Kajuik, Riau. Walaupun nilai query cover tinggi (93–100%) dan E-value sebesar 0,00. Pada keempat barkode DNA yang diteliti, terdapat beberapa perbedaan nukleotida yang disebabkan oleh mutasi insersi-delesi (indel) (6,99%) maupun subtitusi (4,96%). Mutasi indel paling banyak dijumpai pada sekuen trnV-ndhC IGS dan mutasi subtitusi paling banyak terjadi pada sekuen ndhF-rpl32 IGS. Nukleotida kritis yang menjadi penciri bagi Benstonea sp. asal Danau Kajuik, Riau, dijumpai pada sekuen ndhF-rpl32 IGS dan trnQ-5’rps16 IGS. Simpulan, dua sekuen DNA yaitu ndhF-rpl32 IGS dan trnQ-5’rps16 IGS berpotensi menjadi barkode DNA untuk identifikasi tumbuhan ini secara molekuler. Ketersediaan barkode DNA pada database publik sangat diperlukan untuk menunjang identifikasi organisme secara molekuler.AbstractDNA barcode is a piece of short DNA that is developed for molecular identification of organisms. This study aims to analyze four DNA barcodes in pandan plant (Benstonea sp.) from Kajuik Lake, Riau. Methods included DNA extraction, PCR, electrophoresis, purification, sequencing, and bioinformatics analysis. The DNA sequences of atpB-rbcL IGS, trnV-ndhC IGS, ndhF-rpl32 IGS, and trnQ-5’rps16 IGS have been obtained with the length of 812 pb, 924 pb, 952 pb, and 886 pb, respectively. The top accession in BLASTn analysis results showed that there was no accession that had 100% similarity to Benstonea sp. from Kajuik Lake, Riau even though the query cover high (93–100%) and E-value of 0,00. There were some nucleotide variations caused by insertion-deletion (indel) mutation (6,99%) and subtitution (4,96%). Indel was most occur in trnV-ndhC IGS and subtitution in ndhF-rpl32 IGS. Critical nucleotides that were be a characteristic for Benstonea sp. from Kajuik Lake, Riau were seen in ndhF-rpl32 IGS and trnQ-5’rps16 IGS. Conclusion, both of ndhF-rpl32 IGS and trnQ-5’rps16 IGS are potentially as DNA barcodes for molecular identification of this plant. The avaibility of the DNA barcodes is very important to support of organisms molecular identifications.

Analisis Empat Sekuen Barkode DNA Pada Pandan (Benstonea sp.) Asal Danau Kajuik, Riau Dewi Indriyani Roslim; Intan Sari Nuraini; Siti Nurhayati; Ciska Vivian Sianturi; At-Thahirah At-Thahirah; Herman Herman
Al-Kauniyah: Jurnal Biologi Vol 16, No 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.21697

Barkode DNA merupakan sekuen DNA berukuran pendek yang digunakan untuk identifikasi organisme secara molekuler. Penelitian bertujuan menganalisis empat barkode DNA pada tumbuhan pandan (Benstonea sp.) asal Danau Kajuik, Riau. Metode meliputi isolasi DNA, PCR, elektroforesis, purifikasi, sekuesing, serta analisis bioinformatika. Pada penelitian ini telah diperoleh sekuen DNA untuk atpB-rbcL IGS, trnV-ndhC IGS, ndhF-rpl32 IGS, dan trnQ-5’rps16 IGS sepanjang 812 pb, 924 pb, 952 pb, dan 886 pb, secara berturut-turut. Aksesi yang muncul paling atas pada analisis BLASTn pada keempat sekuen tersebut tidak ada yang memiliki kemiripan 100% dengan Benstonea sp. asal Danau Kajuik, Riau. Walaupun nilai query cover tinggi (93–100%) dan E-value sebesar 0,00. Pada keempat barkode DNA yang diteliti, terdapat beberapa perbedaan nukleotida yang disebabkan oleh mutasi insersi-delesi (indel) (6,99%) maupun subtitusi (4,96%). Mutasi indel paling banyak dijumpai pada sekuen trnV-ndhC IGS dan mutasi subtitusi paling banyak terjadi pada sekuen ndhF-rpl32 IGS. Nukleotida kritis yang menjadi penciri bagi Benstonea sp. asal Danau Kajuik, Riau, dijumpai pada sekuen ndhF-rpl32 IGS dan trnQ-5’rps16 IGS. Simpulan, dua sekuen DNA yaitu ndhF-rpl32 IGS dan trnQ-5’rps16 IGS berpotensi menjadi barkode DNA untuk identifikasi tumbuhan ini secara molekuler. Ketersediaan barkode DNA pada database publik sangat diperlukan untuk menunjang identifikasi organisme secara molekuler.AbstractDNA barcode is a piece of short DNA that is developed for molecular identification of organisms. This study aims to analyze four DNA barcodes in pandan plant (Benstonea sp.) from Kajuik Lake, Riau. Methods included DNA extraction, PCR, electrophoresis, purification, sequencing, and bioinformatics analysis. The DNA sequences of atpB-rbcL IGS, trnV-ndhC IGS, ndhF-rpl32 IGS, and trnQ-5’rps16 IGS have been obtained with the length of 812 pb, 924 pb, 952 pb, and 886 pb, respectively. The top accession in BLASTn analysis results showed that there was no accession that had 100% similarity to Benstonea sp. from Kajuik Lake, Riau even though the query cover high (93–100%) and E-value of 0,00. There were some nucleotide variations caused by insertion-deletion (indel) mutation (6,99%) and subtitution (4,96%). Indel was most occur in trnV-ndhC IGS and subtitution in ndhF-rpl32 IGS. Critical nucleotides that were be a characteristic for Benstonea sp. from Kajuik Lake, Riau were seen in ndhF-rpl32 IGS and trnQ-5’rps16 IGS. Conclusion, both of ndhF-rpl32 IGS and trnQ-5’rps16 IGS are potentially as DNA barcodes for molecular identification of this plant. The avaibility of the DNA barcodes is very important to support of organisms molecular identifications.

Pelatihan Pengelolaan Limbah Pelepah Sawit Menjadi Pupuk Kompos dan Pakan Ternak untuk Pemberdayaan Ekonomi Kepada Masyarakat Wilda Susanti; Ramalia Noratama Putri; Fadrul Fadrul; Gusrio Tendra; Herman Herman; Suandi Daulay; Jean Riko Kurniawan Putra; Rinaldy Rinaldy
GERVASI: Jurnal Pengabdian kepada Masyarakat Vol. 7 No. 3 (2023): GERVASI: Jurnal Pengabdian kepada Masyarakat
Publisher : LPPM IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31571/gervasi.v7i3.6714

Tujuan pengabdian pada masyarakat (PkM) ini adalah untuk meningkatkan pengetahuan dan keterampilan mitra dalam mengolah limbah pelepah sawit menjadi pupuk kompos dan pakan ternak. Metode pengabdian yaitu workshop dan pelatihan. Mitra kegiatan pada pengabdian kepada masyarakat kosabangsa tahun 2023 ini adalah Kelompok Tani Bangkit dan BUMKep Maju Bersama Kepenghuluan Teluk Bano 1. Langkah kegiatan meliputi observasi, sosialisasi, implementasi dan evaluasi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan mitra sebesar 66% dan peningkatan keterampilan sebesar 64,9 setelah dilakukan kegiatan. Keberlanjutan program kosabangsa perlu dilakukan dengan baik agar mitra dapat merasakan manfaat dari pelatihan pengelolaan limbah pelepah sawit dan menjadi unit usaha bagi desa Teluk Bano 1.

RESPON GENOTIPE UBI KAYU (Manohot Esculenta Crantz) TERHADAP DOSIS PUPUK KANDANG KOTORAN SAPI TALUK KUANTAN Herman Herman; Dewi Indriyani Roslim; Ingga Yurisna Fitriani
DINAMIKA PERTANIAN Vol. 32 No. 2 (2016): Jurnal Dinamika Pertanian Edisi Agustus 2016
Publisher : UIR Press

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Cassava (Manihot esculenta crantz) is one of the agricultural commoditythat produce tuber for food source in Indonesia. The research objective was to determine the effect of manure on tuber yield and genetic diversity of the five genotypes of cassava Taluk Kuantan. The research was arranged in the randomized block design (RBD) with two factors. The first factor was five cassava genotypes namely: (IY1) cassava pucuk hitam, (IY 2) cassavabangka, (IY3) cassava pulut, (IY4) cassava roti; and (IY5) cassava pucuk hijau. The second factor was the fertilizer of cow manure consists of five doses, namely: P0 (0 kg/plant), P1 (2 kg/plant), P2 (4 kg/plant), P3 (6 kg/plant); and P4 (8 kg/plant). The parameters observed were morphologicaland agronomiccharacters of cassava, such as plant height,number of tubers/plant, tuber diameter, tuber length, and tuber weight/plant. Pulut cassava produced heaviest tuber in the fertilizer with a dose of 4 kg, bangka and pucuk hijau cassavas produced the highest tuber weight in the fertilizer with 6 kg dose.

PEMANFAATAN MULSA ORGANIK Imperata cylindrica (L.), Mucuna bracteata DC. DAN KOMPOS PELEPAH KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP PENGHAMBATAN PERKECAMBAHAN DAN PERTUMBUHAN GULMA Mikania micrantha H.B.K. Siti Fatonah; Herman Herman; Sari Malinda; Mayta Novaliza Isda
DINAMIKA PERTANIAN Vol. 30 No. 3 (2015): Jurnal Dinamika Pertanian Edisi Desember 2015
Publisher : UIR Press

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The aim of this study was to determine effect of the thickness and type of organic mulch (Imperata cylindrica, Mucuna bracteata and compost of palm oil midrib) on germination and growth of Mikania micrantha weeds in polybag at garden. The experiment was designed in Randomized Block Design, with different treatment of three types of mulch, alone or in combination at 2.5 and 5 cm thickness, which contains 11 treatments. Each treatment was repeated seven replications. The results showed that mulch of I. cylindrica, M. bracteata, palm oil compost and their combinations inhibited the germination and growth of M. micrantha weeds. Almost all treatments mulch inhibited germination and growth of M. micrantha to decrease to 100 percent, except for M. bracteata mulch treatment with the reduction reached 97 percent.

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