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All Journal Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi Jurnal Pendidikan, Sains dan Teknologi
Uno, Wirnangsi D.
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Automotive Engineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Environmental Science Languange, Linguistic, Communication & Media Mathematics Social Sciences

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Pelatihan Pembuatan VCO Dengan Metode Fermentasi Untuk Mendukung Kompetensi P5 Siswa Kumaji, Syam S.; Uno, Wirnangsi D.; Aydalin, Regina Valentina; Febriyanti, Febriyanti; Yusuf, Daud; Kobi, Wiwin; Pratama, M.Iqbal Liayong
Lamahu: Jurnal Pengabdian Masyarakat Terintegrasi Vol 4, No 1: February 2025
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/ljpmt.v4i1.31695

Abstract

This activity aims to enhance students' competencies in the field of entrepreneurship through training in the production of Virgin Coconut Oil (VCO) using the fermentation method, as part of the implementation of the Pancasila Student Profile Strengthening Project (P5). The method employed involved educational sessions and hands-on training based on an experiential learning approach, involving 30 eleventh-grade students. The activity included theoretical introduction, practical VCO production, packaging, and marketing planning. Evaluation results indicated a significant increase in students' knowledge from 42% to 85%. Students demonstrated strong practical skills throughout the VCO production process, along with the development of entrepreneurial attitudes such as responsibility, collaboration, and creativity. This training proved effective as a contextual learning medium that reinforces Pancasila values and 21st-century skills.
Isolasi, Identifikasi, dan Uji Efektivitas Metabolit Sekunder Bakteri Endofit Sembung (Blumea balsamifera) Sebagai Biopestisida Terhadap Kutu Kebul (Bemisia tabaci) Timumun, Radina I.; Agustina, Harista; Hasan, Ani M.; Uno, Wirnangsi D.; Retnowati, Yuliana
Jurnal Pendidikan, Sains Dan Teknologi Vol. 5 No. 1 (2026): Januari - Maret
Publisher : CV. ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpst.v5i1.4223

Abstract

Blumea balsamifera contains bioactive metabolites that are presumed to support the presence of endophytic bacteria capable of producing secondary metabolites with pesticidal potential, making this plant a promising source of eco-friendly biopesticides. This study aimed to isolate and identify endophytic bacteria associated with B. balsamifera and to evaluate the insecticidal activity of their secondary metabolites against Bemisia tabaci. Endophytic bacteria were isolated using the Direct Planting and Grinding methods, followed by purification, Gram staining, and metabolite production through liquid culture in Luria-Bertani (LB) medium. The isolated strain was identified as a Gram-negative, short rod-shaped bacterium producing a yellowish supernatant, which was successfully extracted using ethyl acetate to obtain an extracellular metabolite fraction. Toxicity bioassays revealed a clear dose–response pattern, with 24-hour mortality of B. tabaci increasing from 55.00% at 35% concentration to 80.00% at 75% concentration. Probit analysis yielded an LC₅₀ value of 1500.95, while LT₅₀ decreased significantly from 42.26 hours to 15.87 hours as metabolite concentration increased. These findings confirm that endophytic bacteria isolated from B. balsamifera are capable of producing secondary metabolites effective in suppressing B. tabaci populations, highlighting their strong potential for development as environmentally friendly plant-based biopesticides.
Co-Authors Agustina, Harista Aydalin, Regina Valentina Daud Yusuf febriyanti febriyanti HASAN, ANI M. Pratama, M.Iqbal Liayong Syam S. Kumaji Timumun, Radina I. Wiwin Kobi Yuliana Retnowati