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All Journal The Indonesian Biomedical Journal Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat)
Christianto, Antonius
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Biochemistry, Genetics & Molecular Biology Education Health Professions Public Health Social Sciences

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Sosialisasi Pemanfaatan Susu Kambing di SD Islam As-Salam Malang Fatchiyah, Fatchiyah; Wihastuti, Titin Andri; Nurdiana, Nurdiana; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Christianto, Antonius; Hasibuan, Akbar Farid
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 1 No 1 (2021)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2021.001.01.11

Abstract

Sebagian besar masyarakat masih belum mengetahui manfaat yang terkandung pada susu kambing, masyarakat masih menganggap bahwa susu sapi merupakan satu-satunya sumber nutrisi hewani yang memiliki kandungan protein, gizi, dan manfaat yang sangat besar bagi kesehatan. Untuk itu perlu dilakukan sosialisasi tentang pemanfaatan susu kambing karena kurangnya pengetahuan masyarakat sehingga menyebabkan rendahnya daya beli masyarakat. Tujuan dari kegiatan ini adalah untuk menjalin rintisan kerjasama dengan sekolah sebagai salah satu tempat pemasaran produk susu kambing serta orang tua siswa-siswi dalam meningkatkan daya konsumen masyarakat terhadap susu kambing dan memberikan informasi yang berlandaskan data ilmiah tentang manfaat susu kambing. Sosialisasi dilaksanakan melalui pemaparan pemateri tentang manfaat dan pentingnya mengonsumsi susu kambing dalam kehidupan sehari-hari serta pemanfaatan susu kambing menjadi produk nutrisi sehat (susu kambing pasteurisasi). Hasil dari kegiatan sosialisasi adalah para guru dan siswa-siswi bertambah pengetahuannya tentang pentingnya mengonsumsi susu kambing sebagai pangan sehat bernutrisi dan antusias dalam membuat produk-produk sehat berbahan dasar susu kambing.
In silico–guided Design and Endonuclease-Based Functional Validation of sgRNAs Targeting ERBB2 Transmembrane and Kinase Domains Gunsi, Via Susana; Dirgahyu, Anugrah Prima; Meitha, Karlia; Christianto, Antonius; Tan, Marselina Irasonia
The Indonesian Biomedical Journal Vol 18, No 2 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i2.4048

Abstract

BACKGROUND: Erythroblastic oncogene B2 (ERBB2) overexpression promotes breast cancer progression through activation of phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) and mitogen-activated protein kinase (MAPK) signaling pathways. Although CRISPR–Cas9 enables precise gene disruption, sgRNA efficiency is strongly influenced by target sequence and structural features, necessitating careful in silico design and validation. This study aimed to design and evaluate sgRNAs targeting the transmembrane- and tyrosine kinase–encoding regions of ERBB2.METHODS: Candidate sgRNAs were selected using CHOPCHOP, CRISPOR, and CRISPR RGEN, followed by secondary structure analysis with RNAfold to assess folding stability and scaffold integrity. sgRNAs were synthesized via in vitro transcription and assembled with Cas9 protein to form ribonucleoprotein (RNP) complexes. In vitro endonuclease assays were performed using PCR-amplified ERBB2 fragments derived from SK-OV3 genomic DNA, corresponding to exon 22 (523 bp) and exon 25 (933 bp). sgRNAs targeting eIF4E1 served as positive controls.RESULTS: Two sgRNAs meeting all in silico criteria were selected. Secondary structure prediction confirmed that both sgRNAs possessed essential structural elements required for Cas9 interaction including the repeat–anti-repeat region and three stem–loop structures. In vitro endonuclease assays demonstrated that the sgRNA targeting exon 22 of ERBB2 successfully cleaved its target DNA, producing fragments of 266 bp and 257 bp. Similar cleavage activity was observed in the two control sgRNAs, which generated fragments of 384 bp and 303 bp, and 486 bp and 201 bp, respectively. In contrast, the sgRNA targeting exon 25 of ERBB2 exhibited no detectable cleavage activity.CONCLUSION: The sgRNA targeting exon 22 of ERBB2 demonstrated effective DNA cleavage activity in vitro, whereas the sgRNA targeting exon 25 showed no endonuclease activity.KEYWORDS: CRISPR–Cas9, endonuclease, ERBB2, in vitro, breast cancer, RNP, sgRNA
Co-Authors Dirgahyu, Anugrah Prima fatchiyah . Gunsi, Via Susana Hasibuan, Akbar Farid Lidwina Faraline Triprisila Meitha, Karlia Nurdiana Nurdiana Rista Nikmatu Rohmah, Rista Nikmatu Tan, Marselina Irasonia Titin Andri Wihastuti