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All Journal The Indonesian Biomedical Journal Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat)
Christianto, Antonius
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Biochemistry, Genetics & Molecular Biology Education Health Professions Public Health Social Sciences

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Sosialisasi Pemanfaatan Susu Kambing di SD Islam As-Salam Malang Fatchiyah, Fatchiyah; Wihastuti, Titin Andri; Nurdiana, Nurdiana; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Christianto, Antonius; Hasibuan, Akbar Farid
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 1 No 1 (2021)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2021.001.01.11

Abstract

The use of milk is very popular, both in the form of the milk itself and other processed forms such as yogurt, cheese, kefir, and others. Most people still think that cow's milk is the only source of animal nutrition that contains protein, nutrition and enormous health benefits. Not many people know that goat's milk has the same, even better, benefits than cow's milk. Community service through the dissemination of pasteurized goat's milk is carried out. Lack of public knowledge about the health benefits and nutritional content of goat's milk causes people's purchasing power is still very low. In addition, considering the importance of the benefits of goat's milk, we need an activity that is effective and informative, educative and persuasive in the context of introducing its products. The purpose of this activity is to establish a pioneering collaboration with schools as one of the marketing sites for goat's milk products and parents of students in increasing the consumer's power of the community towards goat's milk and providing information based on scientific data about the benefits of goat's milk. The socialization was carried out starting with the presentation of the presenters about the benefits and importance of consuming goat's milk in daily life, the utilization of goat's milk into healthy nutritional products (pasteurized goat's milk). The results that can be conveyed from the socialization activities are that teachers and students increase their knowledge of the importance of consuming goat's milk as healthy food and are enthusiastic in making healthy products made from goat's milk.
In silico–guided Design and Endonuclease-Based Functional Validation of sgRNAs Targeting ERBB2 Transmembrane and Kinase Domains Gunsi, Via Susana; Dirgahyu, Anugrah Prima; Meitha, Karlia; Christianto, Antonius; Tan, Marselina Irasonia
The Indonesian Biomedical Journal Vol 18, No 2 (2026)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v18i2.4048

Abstract

BACKGROUND: Erythroblastic oncogene B2 (ERBB2) overexpression promotes breast cancer progression through activation of phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) and mitogen-activated protein kinase (MAPK) signaling pathways. Although CRISPR–Cas9 enables precise gene disruption, sgRNA efficiency is strongly influenced by target sequence and structural features, necessitating careful in silico design and validation. This study aimed to design and evaluate sgRNAs targeting the transmembrane- and tyrosine kinase–encoding regions of ERBB2.METHODS: Candidate sgRNAs were selected using CHOPCHOP, CRISPOR, and CRISPR RGEN, followed by secondary structure analysis with RNAfold to assess folding stability and scaffold integrity. sgRNAs were synthesized via in vitro transcription and assembled with Cas9 protein to form ribonucleoprotein (RNP) complexes. In vitro endonuclease assays were performed using PCR-amplified ERBB2 fragments derived from SK-OV3 genomic DNA, corresponding to exon 22 (523 bp) and exon 25 (933 bp). sgRNAs targeting eIF4E1 served as positive controls.RESULTS: Two sgRNAs meeting all in silico criteria were selected. Secondary structure prediction confirmed that both sgRNAs possessed essential structural elements required for Cas9 interaction including the repeat–anti-repeat region and three stem–loop structures. In vitro endonuclease assays demonstrated that the sgRNA targeting exon 22 of ERBB2 successfully cleaved its target DNA, producing fragments of 266 bp and 257 bp. Similar cleavage activity was observed in the two control sgRNAs, which generated fragments of 384 bp and 303 bp, and 486 bp and 201 bp, respectively. In contrast, the sgRNA targeting exon 25 of ERBB2 exhibited no detectable cleavage activity.CONCLUSION: The sgRNA targeting exon 22 of ERBB2 demonstrated effective DNA cleavage activity in vitro, whereas the sgRNA targeting exon 25 showed no endonuclease activity.KEYWORDS: CRISPR–Cas9, endonuclease, ERBB2, in vitro, breast cancer, RNP, sgRNA
Co-Authors Dirgahyu, Anugrah Prima fatchiyah . Gunsi, Via Susana Hasibuan, Akbar Farid Lidwina Faraline Triprisila Meitha, Karlia Nurdiana Nurdiana Rista Nikmatu Rohmah, Rista Nikmatu Tan, Marselina Irasonia Titin Andri Wihastuti