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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Indonesian Journal of Biotechnology Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat)
Rista Nikmatu Rohmah, Rista Nikmatu
Departement of Biology, Faculty of Mathematic and Natural Science, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Public Health Social Sciences

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Cloning and Expression of hGAD65 Gene in E. Coli BL21 Rohmah, Rista Nikmatu; Widyasari, Soraya; ., Aulanni’am; ., Fatchiyah
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.75 KB)

Abstract

The aim of this study is to construct the hGAD65 gene and to identify the hGAD65 clone by using PCR & RFLP. The samples were derived from normal person & DM patient’s blood. Blood DNA was isolated by salting out method and then amplified by PCR with a pair of specific primer, GAD65-F-BamH1-807 & GAD65-R-Xho1-945. The PCR-product was cloned into vector pET-28a and the pET28a-hGAD65-clone was transformed into E.coli BL21 competent cells. The pET28a-hGAD65-clone was confirmed by PCR and RFLP by BamH1 & XhoI. The PCR product of pET28a-hGAD65-clone was one band of 159bp and has two bands 5.3 kb and 159 bp by RFLPwith both restriction enzymes. The GAD65 protein is expressed in 65kD of pET28a-hGAD65-clone. PET28a-hGAD65-clone was able to recognize by gold standard monoclonal antibody specifically. These results indicated that the hGAD65 gene inserted into pET28a properly and provided the GAD65 protein expression.Key words: hGAD65, PCR, pET-28a, RFLP
Level of mRNA Insulin Gene and Blood Glucose STZ-Induced Diabetic Rat are Improved by Glucomannan of Amorphophallus muelleri Blume from East Java Forest Indonesia Fatchiyah, Fatchiyah; Nurmasari, Damai Aulia; Masruro, Nuri; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Mulyati, Mulyati; Yamada, Takahisa; Ohta, Takashi
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.089 KB) | DOI: 10.11594/jtls.09.02.05

Abstract

Diabetes mellitus is one of metabolic disorder with high level of blood sugar glucose and reduces pancreatic-insulin levels that are altered by unhealthy food and lifestyle. To manage the blood glucose and insulin level, we need the diabetes dietary management related with insulin gene cascade. The purpose of this study was to investigate the influence glucomannan fiber to mRNA level of the insulin gene and genes cascade expression of target cells of type 1 diabetic rat. Three-month-old male (Rattus norvegicus strain Wistar) were fed with fiber of glucomannan of Amorphophallus muelleri Blume with dosage 0,06g/kg BW and 0.12g/kg BW every day during one month. RNA dot blotting with specific cDNA probe was performed mRNA level of insulin gene. The result of this study showed that glucomannan could reduce of body weight and blood glucose level significantly. The mRNA level of insulin gene of diabetic rats-treated 0.12g/kg BW glucomannan fiber dosage was the highest significance level among of all groups. In histopathological analysis, glucomannan was increasing significantly in IRS-1 level expression proliferate properly and consistently increasing of the PI3-K expression level in treated diabetic rats. This study indicates that the 0.12g/kg BW glucomannan was optimum dosage that effectively as role for alternative high-fiber therapy of type 1 diabetic through insulin receptor tyrosine kinase pathway. 
Sosialisasi Pemanfaatan Susu Kambing di SD Islam As-Salam Malang Fatchiyah, Fatchiyah; Wihastuti, Titin Andri; Nurdiana, Nurdiana; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Christianto, Antonius; Hasibuan, Akbar Farid
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 1 No 1 (2021)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2021.001.01.11

Abstract

The use of milk is very popular, both in the form of the milk itself and other processed forms such as yogurt, cheese, kefir, and others. Most people still think that cow's milk is the only source of animal nutrition that contains protein, nutrition and enormous health benefits. Not many people know that goat's milk has the same, even better, benefits than cow's milk. Community service through the dissemination of pasteurized goat's milk is carried out. Lack of public knowledge about the health benefits and nutritional content of goat's milk causes people's purchasing power is still very low. In addition, considering the importance of the benefits of goat's milk, we need an activity that is effective and informative, educative and persuasive in the context of introducing its products. The purpose of this activity is to establish a pioneering collaboration with schools as one of the marketing sites for goat's milk products and parents of students in increasing the consumer's power of the community towards goat's milk and providing information based on scientific data about the benefits of goat's milk. The socialization was carried out starting with the presentation of the presenters about the benefits and importance of consuming goat's milk in daily life, the utilization of goat's milk into healthy nutritional products (pasteurized goat's milk). The results that can be conveyed from the socialization activities are that teachers and students increase their knowledge of the importance of consuming goat's milk as healthy food and are enthusiastic in making healthy products made from goat's milk.
Co-Authors Aulanni'am, Aulanni'am Christianto, Antonius fatchiyah . Hasibuan, Akbar Farid Lidwina Faraline Triprisila Masruro, Nuri Mulyati Mulyati Nurdiana Nurdiana Nurmasari, Damai Aulia Ohta, Takashi Soraya Widyasari, Soraya Titin Andri Wihastuti Yamada, Takahisa