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Studi Toksisitas Bacillus thuringiensis Isolat Lokal Jawa Timur Berdasarkan Ketinggian Tempat Terhadap Larva Aedes aegypti Triprisila, Lidwina Faraline; Suharjono, Suharjono; Gama, Zulfaidah Penata
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.984 KB)

Demam Berdarah Dengue (DBD) masih menjadi masalah bagi kesehatan masyarakat dan merupakan penyakit endemis yang menyebabkan angka kematiannya tinggi hampir di seluruh provinsi di Indonesia.Â Pada kurun empat tahun terakhir Jawa Timur memiliki kasus DBD paling tinggi. Ketinggian tempat merupakan faktor yang membatasi penyebaran Aedes aegypti sebagai vektor epidemik penyakit demam berdarah. Salah satu cara yang aman untuk memberantas nyamuk tersebut adalah dengan Bacillus thuringiensis. Tujuan penelitian ini untuk mendapatkan isolat dan menguji toksisitas Bacillus thuringiensis yang efektif dalam membunuh larva Aedes aegypti dari beberapa ketinggian tempat di Jawa Timur. Sampel sedimen dan air diambil dari dataran tinggi Blitar dan Bondowoso, dataran sedang di Bangkalan, Ponorogo, Madiun dan Tulungagung serta dataran rendah di Lamongan dan Surabaya. Bacillus thuringiensis diisolasi dengan media selektif. Isolat yang didapatkan diamati karakteristik fenotip (Profil Matching Method), kemudian dilakukan pengujian toksisitas pada larva nyamuk Aedes aegypti instar III. Persentase mortalitas larva dianalisis probit (LC50), ragam (ANOVA) dan uji t (t-test). Dua dari 28 isolat Bacillus thuringiensis yang didapatkan yaitu SK.T dan Mdn I TK2 mampu membunuh larva Aedes aegypti lebih dari 50 %. Isolat Mdn I TK2 pada umur biakan 48 jam dan isolat SK.T pada umur biakan 24 jam pada waktu pendedahan 72 jam secara berturut-turut mampu membunuh larva Aedes aegypti sebesar 100 % dan 68,3 %. Isolat Mdn I TK2 lebih efektif membunuh larva Aedes aegypti dibandingkan isolat SK.T dengan nilai LC50-72jam sebesar 2,17Ã107 sel/ml.

Level of mRNA Insulin Gene and Blood Glucose STZ-Induced Diabetic Rat are Improved by Glucomannan of Amorphophallus muelleri Blume from East Java Forest Indonesia Fatchiyah, Fatchiyah; Nurmasari, Damai Aulia; Masruro, Nuri; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Mulyati, Mulyati; Yamada, Takahisa; Ohta, Takashi
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Diabetes mellitus is one of metabolic disorder with high level of blood sugar glucose and reduces pancreatic-insulin levels that are altered by unhealthy food and lifestyle. To manage the blood glucose and insulin level, we need the diabetes dietary management related with insulin gene cascade. The purpose of this study was to investigate the influence glucomannan fiber to mRNA level of the insulin gene and genes cascade expression of target cells of type 1 diabetic rat. Three-month-old male (Rattus norvegicus strain Wistar) were fed with fiber of glucomannan of Amorphophallus muelleri Blume with dosage 0,06g/kg BW and 0.12g/kg BW every day during one month. RNA dot blotting with specific cDNA probe was performed mRNA level of insulin gene. The result of this study showed that glucomannan could reduce of body weight and blood glucose level significantly. The mRNA level of insulin gene of diabetic rats-treated 0.12g/kg BW glucomannan fiber dosage was the highest significance level among of all groups. In histopathological analysis, glucomannan was increasing significantly in IRS-1 level expression proliferate properly and consistently increasing of the PI3-K expression level in treated diabetic rats. This study indicates that the 0.12g/kg BW glucomannan was optimum dosage that effectively as role for alternative high-fiber therapy of type 1 diabetic through insulin receptor tyrosine kinase pathway.Â

Studi Toksisitas Bacillus thuringiensis Isolat Lokal Jawa Timur Berdasarkan Ketinggian Tempat Terhadap Larva Aedes aegypti Lidwina Faraline Triprisila; Suharjono Suharjono; Zulfaidah Penata Gama
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Demam Berdarah Dengue (DBD) masih menjadi masalah bagi kesehatan masyarakat dan merupakan penyakit endemis yang menyebabkan angka kematiannya tinggi hampir di seluruh provinsi di Indonesia. Pada kurun empat tahun terakhir Jawa Timur memiliki kasus DBD paling tinggi. Ketinggian tempat merupakan faktor yang membatasi penyebaran Aedes aegypti sebagai vektor epidemik penyakit demam berdarah. Salah satu cara yang aman untuk memberantas nyamuk tersebut adalah dengan Bacillus thuringiensis. Tujuan penelitian ini untuk mendapatkan isolat dan menguji toksisitas Bacillus thuringiensis yang efektif dalam membunuh larva Aedes aegypti dari beberapa ketinggian tempat di Jawa Timur. Sampel sedimen dan air diambil dari dataran tinggi Blitar dan Bondowoso, dataran sedang di Bangkalan, Ponorogo, Madiun dan Tulungagung serta dataran rendah di Lamongan dan Surabaya. Bacillus thuringiensis diisolasi dengan media selektif. Isolat yang didapatkan diamati karakteristik fenotip (Profil Matching Method), kemudian dilakukan pengujian toksisitas pada larva nyamuk Aedes aegypti instar III. Persentase mortalitas larva dianalisis probit (LC50), ragam (ANOVA) dan uji t (t-test). Dua dari 28 isolat Bacillus thuringiensis yang didapatkan yaitu SK.T dan Mdn I TK2 mampu membunuh larva Aedes aegypti lebih dari 50 %. Isolat Mdn I TK2 pada umur biakan 48 jam dan isolat SK.T pada umur biakan 24 jam pada waktu pendedahan 72 jam secara berturut-turut mampu membunuh larva Aedes aegypti sebesar 100 % dan 68,3 %. Isolat Mdn I TK2 lebih efektif membunuh larva Aedes aegypti dibandingkan isolat SK.T dengan nilai LC50-72jam sebesar 2,17×107 sel/ml.

Oral Administration of The Hypercholesterol Rat Feed Formula to Making The Animal Dyslipidemia Model on Sprague Dawley Rats Fatchiyah Fatchiyah; Eko Suyanto; Rista Nikmatu Rohmah; Lidwina Faraline Triprisila; Hazna Noor Meidinna; Dewi Ratih Tirto Sari; Iva Himmatul Aliyah
Biotropika: Journal of Tropical Biology Vol 9, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2021.009.02.08

The aim of this study was to make animal dyslipidemia models in Sprague Dawley strains induced by a high fat goat diet formula as hypercholesterol feed for two months. The experimental animal used in this study was 30 male rats (Rattus norvegicus strain Sprague Dawley) with an age of 2-3 months with an average body weight of 150g. Animal models are divided into two groups consisting of a control group without additional diet and dyslipidemia group given food consumption goat hypercholesterolemia with high-fat diet formula orally every day for two months. Physiological characteristics of dyslipidemia SD rats had higher body weight, increased food consumption and fecal weight, and decreased water intake and urine volume than the control group. Total cholesterol, triglyceride, and LDL-cholesterol levels increased, while HDL-cholesterol levels did not change in the dyslipidemia rats group compared to the control group. The conclusion of this study indicated that the hypercholesterol diet formula with a high composition of goat fat was successfully induced the SD rats to become dyslipidemia model rat with specific hypercholerol characteristics.

Antimicrobial Activity of Ferulic Acid in Indonesian Purple Rice through Toll-like Receptor Signaling Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Triprisila, Lidwina Faraline; Fatchiyah, Fatchiyah
Makara Journal of Science Vol. 25, No. 4
Publisher : UI Scholars Hub

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Purple rice is a potential source of ferulic acid, which has antimicrobial properties. However, the inhibitory mechanism of ferulic acid on the growth of bacteria, particularly Salmonella and Listeria, has not been elucidated. This study aimed to determine the bioactivity of ferulic acid from purple rice as an antimicrobial agent against Salmonella typhimurium and Listeria monocytogenes using in vitro and in silico analyses. The antimicrobial activity of a purple rice ferulic acid extract was tested using the agar well diffusion method. Its effect on bacterial cells was observed using scanning electron microscopy. Ferulic acid was confirmed to have antimicrobial properties using in silico software to attenuate the binding of bacterial virulence factors (lipoproteins, lipopolysaccharides, and flagellins) to Toll-like receptors (TLRs) and to prevent interactions with peptidoglycans. The purple rice ferulic acid extract inhibited bacterial growth. The inhibitory effects included induction of a biofilm and shrinkage of S. Typhimurium, as well as osmotic lysis of L. monocytogenes. This activity was supported by the ability of ferulic acid to inhibit the binding of bacterial virulence factors with TLRs and block bacterial peptidoglycans. These findings indicate that the purple rice ferulic acid extract acts as antimicrobial, both directly to bacterial cells and indirectly through TLRs. We conclude that ferulic acid from Indonesian purple rice has a biological function as an antimicrobial agent.

Level of mRNA Insulin Gene and Blood Glucose STZ-Induced Diabetic Rat are Improved by Glucomannan of Amorphophallus muelleri Blume from East Java Forest Indonesia Fatchiyah Fatchiyah; Damai Aulia Nurmasari; Nuri Masruro; Rista Nikmatu Rohmah; Lidwina Faraline Triprisila; Mulyati Mulyati; Takahisa Yamada; Takashi Ohta
Journal of Tropical Life Science Vol. 9 No. 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.02.05

Diabetes mellitus is one of metabolic disorder with high level of blood sugar glucose and reduces pancreatic-insulin levels that are altered by unhealthy food and lifestyle. To manage the blood glucose and insulin level, we need the diabetes dietary management related with insulin gene cascade. The purpose of this study was to investigate the influence glucomannan fiber to mRNA level of the insulin gene and genes cascade expression of target cells of type 1 diabetic rat. Three-month-old male (Rattus norvegicus strain Wistar) were fed with fiber of glucomannan of Amorphophallus muelleri Blume with dosage 0,06g/kg BW and 0.12g/kg BW every day during one month. RNA dot blotting with specific cDNA probe was performed mRNA level of insulin gene. The result of this study showed that glucomannan could reduce of body weight and blood glucose level significantly. The mRNA level of insulin gene of diabetic rats-treated 0.12g/kg BW glucomannan fiber dosage was the highest significance level among of all groups. In histopathological analysis, glucomannan was increasing significantly in IRS-1 level expression proliferate properly and consistently increasing of the PI3-K expression level in treated diabetic rats. This study indicates that the 0.12g/kg BW glucomannan was optimum dosage that effectively as role for alternative high-fiber therapy of type 1 diabetic through insulin receptor tyrosine kinase pathway.Â

Sosialisasi Pemanfaatan Susu Kambing di SD Islam As-Salam Malang Fatchiyah, Fatchiyah; Wihastuti, Titin Andri; Nurdiana, Nurdiana; Rohmah, Rista Nikmatu; Triprisila, Lidwina Faraline; Christianto, Antonius; Hasibuan, Akbar Farid
TRI DHARMA MANDIRI: Dissemination and Downstreaming of Research to the Community (Journal of Community Engagement) Vol 1 No 1 (2021)
Publisher : SMONAGENES Research Center, Univeritas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtridharma.2021.001.01.11

Sebagian besar masyarakat masih belum mengetahui manfaat yang terkandung pada susu kambing, masyarakat masih menganggap bahwa susu sapi merupakan satu-satunya sumber nutrisi hewani yang memiliki kandungan protein, gizi, dan manfaat yang sangat besar bagi kesehatan. Untuk itu perlu dilakukan sosialisasi tentang pemanfaatan susu kambing karena kurangnya pengetahuan masyarakat sehingga menyebabkan rendahnya daya beli masyarakat. Tujuan dari kegiatan ini adalah untuk menjalin rintisan kerjasama dengan sekolah sebagai salah satu tempat pemasaran produk susu kambing serta orang tua siswa-siswi dalam meningkatkan daya konsumen masyarakat terhadap susu kambing dan memberikan informasi yang berlandaskan data ilmiah tentang manfaat susu kambing. Sosialisasi dilaksanakan melalui pemaparan pemateri tentang manfaat dan pentingnya mengonsumsi susu kambing dalam kehidupan sehari-hari serta pemanfaatan susu kambing menjadi produk nutrisi sehat (susu kambing pasteurisasi). Hasil dari kegiatan sosialisasi adalah para guru dan siswa-siswi bertambah pengetahuannya tentang pentingnya mengonsumsi susu kambing sebagai pangan sehat bernutrisi dan antusias dalam membuat produk-produk sehat berbahan dasar susu kambing.

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