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Identifikasi Eschericia coli pada Minuman Es Kelapa Muda dengan Metode Most Probable Number (MPN) di Kecamatan Banjarmasin Utara Hajrah Hidriya; Siti Saidah; Faridah Ariyani
Jurnal Kesehatan Indonesia Vol 12 No 1 (2021): November 2021
Publisher : HB PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33657/jurkessia.v12i1.563

Abstract

Young coconut ice drink is a type of traditional drink containing good nutrition for the growth of microorganisms such as bacteria. One of the causes of young coconut ice contamination is Escherichia coli. Escherichia coli is a gram-negative bacterium and is a factor causing disease, especially diarrhea. This study aimed to determine the identification of young coconut ice drink with the most probable number (MPN) method. The examination used the MPN method in three stages. The initial test used lactose broth as a fertilizer. If the result was positive, it continued to EC Broth media as a confirmation test. If the result in EC Broth media was positive, it continued to EMB media for the perfection test. The results of the study using nine samples of young coconut ice showed that six samples were positively contaminated by E.coli (67%) and three samples were not contaminated by E.coli (33%). Based on the results of the study, it could be concluded that young coconut ice drinks sold in the North Banjarmasin Subdistrict were mostly contaminated with E.coli bacteria that were declared not to meet the standard requirements for food/beverage sales according to the Food and Drug Supervisory Agency (BPOM ISBN 978-602-3665-11-26) in 2012.
PERBANDINGAN EFEKTIVITAS EKSTRAK DAUN KERSEN (Mutingia calabura) DAN DAUN AKASIA (Acacia mangium) SEBAGAI LARVASIDA TERHADAP JENTIK NYAMUK Aedes aegypti (L.) DI WILAYAH BANJARMASIN Hajrah Hidriya; Wulan Pertiwi; Yuliana Salman
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 2 (2022): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i2.2893

Abstract

Dengue fever (dengue) is a dangerous and even deadly disease caused by the Aedes aegypti mosquito as the vector of the disease. One way to prevent dengue fever (dengue) is the use of larvicides to inhibit and kill Aedes aegypti larvae. In this case, it is necessary to use one part of the plant, namely Kersen leaves (Mutingia calabura) and Acacia leaves (Acacia mangium). This study aims to compare the effectiveness of the effect of the bioactive compounds contained in extracts of Kersen leaves (Mutingia calabura) and Acacia leaves (Acacia mangium) as larvicides. The plant extraction method is simplicia. The prepared leaf samples were washed thoroughly and dried for ± 2 weeks in a closed state from the sun. The dried leaf samples were then mashed with a dry blender to obtain powder (dry weight), then macerated with ethanol solvent with a concentration of 0.1%, 0.5% and 1%. Based on the results of the research conducted, there was a significant effect of giving cherry leaf extract and acacia leaves from each concentration tested after 24 hours of treatment, with the highest number of larvae deaths at a concentration of 1%. However, in this case there was no significant difference between the administration of the two extracts as Aedes aegypti larvicide
Identifikasi Eschericia coli pada Minuman Es Kelapa Muda dengan Metode Most Probable Number (MPN) di Kecamatan Banjarmasin Utara Hajrah Hidriya; Siti Saidah; Faridah Ariyani
Jurnal Kesehatan Indonesia Vol 12 No 1 (2021): November 2021
Publisher : HB PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Young coconut ice drink is a type of traditional drink containing good nutrition for the growth of microorganisms such as bacteria. One of the causes of young coconut ice contamination is Escherichia coli. Escherichia coli is a gram-negative bacterium and is a factor causing disease, especially diarrhea. This study aimed to determine the identification of young coconut ice drink with the most probable number (MPN) method. The examination used the MPN method in three stages. The initial test used lactose broth as a fertilizer. If the result was positive, it continued to EC Broth media as a confirmation test. If the result in EC Broth media was positive, it continued to EMB media for the perfection test. The results of the study using nine samples of young coconut ice showed that six samples were positively contaminated by E.coli (67%) and three samples were not contaminated by E.coli (33%). Based on the results of the study, it could be concluded that young coconut ice drinks sold in the North Banjarmasin Subdistrict were mostly contaminated with E.coli bacteria that were declared not to meet the standard requirements for food/beverage sales according to the Food and Drug Supervisory Agency (BPOM ISBN 978-602-3665-11-26) in 2012.
ANALISIS GC-MS EKSTRAK DAUN KERSEN (Muntingia calabura) TERHADAP MORTALITAS LARVA AEDES AEGYPTII Aprilda Fatma Dewi; Muhammad Raihan Fadil; Restiana Aprilita; Rosintan Enggar Wahyuningdiah; Siti Raudhah Salsabila; Hajrah Hidriya
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 15 No 2 (2023): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v15i2.2357

Abstract

Demam berdarah dengue merupakan penyakit yang diakibatkan oleh virus Dengue dan ditularkan melewati gigitan nyamuk Aedes aegyptii. Secara epidemologi, penyakit demam berdarah di Indonesia memang cenderung tinggi. Beberapa upaya telah dilakukan dalam mengatasi penyebaran penyakit DBD namun sampai sekarang masih belum optimal dalam melaksanakan pemberantasan penyakit tersebut. Salah satu metode penghindaran penyakit demam berdarah (dengue) yaitu larvasida alami. Dalam perihal itu pentingnya pendayagunaan suatu bagian dari tumbuhan seperti daun kersen (Mutingia calabura). Riset ini bertujuan untuk pengaruh dan potensi kandungan ekstrak daun Kersen (Mutingia calabura) sebagai larvasida alami. Jenis penelitian menggunakan rancangan acak lengkap (RAL) pada pola post test only control group design. Tahapan pengerjaan mulai dari pengambilan daun kersen, pembuatan ekstrak, perkembangbiakkan larva, uji fitokimia, dilanjutkan dengan uji efektivitas ekstrak daun kersen, kemudian analisis/ uji GCMS untuk mengetahui kandungan pada ekstrak daun kersen (Muntingia calabura). Hasil penelitian didapatkan bahwa kematian larva yang sangat signifikan pada konsentrasi 1,5% dan 2% sedangkan konsentrasi 0,5% menunjukan bahwa berpengaruh terhadap kematian larva sehingga pada konsentrasi tersebut efektif dalam menghambat perkembangbiakan larva. Berdasarkan hasil penelitian dapat disimpulkan bahwa adanya pengaruh dari kandungan senyawa yang terdapat pada ekstrak daun kersen (Muntingia calabura). Hasil analisis GC-MS menunjukkan persen area yang paling tinggi yaitu pada peak nomor 49 sebesar 24.44%, yaitu senyawa d-Tocopherol, d-Tocopherol-O-acetyl, Pregn, acetyloxy, dihydroxy, lactone yang merupakan turunan dari saponin.