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Hubungan Ekspresi Imunohistokimia Protein Gene Product (PGP9.5) dengan Derajat Histopatologi Tipe Adenokarsinoma Serosum dan Musinosum Ovarium Radita Nur Anggraini Ginting; T. Ibnu Alferraly; Soekimin Soekimin
Majalah Patologi Indonesia Vol 26 No 1 (2017): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.353 KB)

Latar belakang Kanker ovarium merupakan kanker ginekologi yang dijumpai hampir 30% dari semua kanker organ reproduksi. Tipe paling umum dijumpai adalah kanker ovarium yang berasal dari epitel permukaan ovarium. Differensiasi menjadi jenis sel-sel epitel pada kanker ovarium jenis epitel berada pada kontrol yang sama dan nantinya akan menentukan jenis epitel pada organ sistem reproduksi wanita selama perkembangan embrionik. Protein gene product (PGP9.5) merupakan suatu peptide spesifik pada neuron yang menghilangkan ubiquitin dari protein-protein ubiquinated dan dapat berperan sebagai promoter hipermetilasi yang penting dalam gen transkripsi pada kanker. Tujuan penelitian ini untuk melihat hubungan ekspresi imunohistokimia PGP9.5 pada berbagai derajat histopatologi kanker ovarium tipe adenokarsinoma serosum dan adenokarsinoma musinosum. Metode Penelitian ini bersifat deskriptif analitik dengan pendekatan penelitian cross sectional. Penelitian ini dilakukan dengan pengambilan dan pengumpulan blok parafin jaringan ovarium sebanyak 45 sampel terdiri dari 22 sampel adenokarsinoma serosum dan 13 sampel adenokarsinoma musinosum, dipulas dengan imunohistokimia PGP9.5 dan dilakukan interpretasi dengan menilai intensitas kualitas tampilan warna pada sel tumor. Hasil Berdasarkan analisa dengan uji Kruskal-Wallis didapati 26 sampel yang terpulas negatif dengan imunohistokimia PGP9.5 terdapat 13 kasus dengan derajat differensiasi well differentiated (50%), 6 kasus (23,1%) moderately differentiated dan 7 kasus (26,9%) poorly differentiated. Pada 5 sampel yang terekspresi lemah tedapat 4 kasus (80%) dengan well differentiated dan 1 kasus (20%) dengan poorly differentiated. Pada 4 kasus terekspresi sedang, terdapat 2 kasus (50%) pada well differentiated, 1 kasus (25%) pada moderately differentiated dan 1 kasus (25%) pada poorly differentiated. Pada 10 sampel yang terekspresi kuat, terdapat 2 kasus(20%) pada well differentiated dan 8 kasus (80%) pada poorly differentiated Kesimpulan Tidak ada hubungan ekspresi PGP9.5 dengan derajat histopatologi kanker ovarium tipe adenokarsinoma serosum dan adenokarsinoma musinosum (p-value >0,05). Kata kunci: adenokarsinoma musinosum, adenokarsinoma serosum, imunohistokimia, kanker ovarium, protein gene product (PGP9.5).

Hubungan Ekspresi CD117 dan Epidermal Growth Factor Receptor (EGFR) terhadap Grading Histopatologi Adenoid Cystic Carcinoma pada Kelenjar Liur Ren Astrid Allail Siregar; T. Ibnu Alferraly; Delyuzar
Majalah Patologi Indonesia Vol 29 No 1 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundTend to have adenoid cystic carcinoma (AdCC) is one of malignant epithelial tumor of the salivary gland poor prognosis, localrecurrence and metastases. Histopathological grading system according Weert, et al. observed from presence or absence solidcomponent of AdCC. CD117 is a transmembrane tyrosine kinase receptor usually strong expression in AdCC. EGFR belongs to theErbB family of receptor tyrosine kinases associated with cell proliferation, angiogenesis and metastases. Patients with advanceddisease may benefit from targeted therapy besides conventional management. Expression of CD117 and EGFR may give proposaltherapy through difference histology pattern of AdCC based on histopathological grading.MethodsThis is an analytical descriptive study with cross sectional approach. Formalin-fixed paraffin-embedded sections from 35 adenoidcystic carcinomas were obtained for histopathological grading and for immunostained of CD117 and EGFR using monoclonalantibodies. The staining intensity and percentage of cells with membranous and cytoplasmic of CD117 and EGFR expression werescored and then grouped according to the parameters of the Allred scoring system.ResultsChi-square test between CD117, EGFR expression and histopathological grading of AdCC showed no sigficant differences.Besides, McNemar test between CD117 and EGFR expression and histopathological grading AdCC showed significant differences,p-value=0.03 (p<0.05).ConclusionsCD117 and EGFR could be used to detected salivary gland AdCC

Knowledge Level of Doctors in USU Medical School Regarding Doctor-Patient Communication Chailes, Andre; T. Ibnu Alferraly
SCRIPTA SCORE Scientific Medical Journal Vol. 4 No. 1 (2022): SCRIPTA SCORE Scientific Medical Journal
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/scripta.v4i1.8867

Background. While carrying out its duties, doctors certainly cannot be separated from the act of communicating with patients. Doctor-patient communication is defined as ongoing communication between doctors, as a medic, with the patient as the treated person. This communication skills are important and therefore must be mastered by doctors. But before that, doctors must have adequate knowledge of how to communicate properly and effectively with patients. Lack of this communication skill will be a serious problem so it should not be ignored. Objective. To determine the doctors level of knowledge about doctor-patient communication. Methods. This study was conducted in descriptive with cross sectional approach. The data were collected by distributing the questionnaire of 15 questions to 71 doctors who were still actively teaching at Faculty of Medicine, Universitas Sumatera Utara. The collected data then analyzed using SPSS computer program. Results. The results showed that most of the doctor that work on Faculty of Medicine, Universitas Sumatera Utara, 83.1% of the samples were in good category of knowledge, 14.1% of the samples were in sufficient category of knowledge, the rest 2.8% of the samples were in low category of knowledge. Conclusion. Majority of the doctor have a good level of knowledge about doctor-patient communication. Keyword: Communication, Doctor-Patient, Knowledge, Skills

Hubungan Ekspresi Imunohistokimia Protein Gene Product (PGP9.5) dengan Derajat Histopatologi Tipe Adenokarsinoma Serosum dan Musinosum Ovarium Radita Nur Anggraini Ginting; T. Ibnu Alferraly; Soekimin Soekimin
Majalah Patologi Indonesia Volume 26 No. 1, Januari 2017
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Latar belakang Kanker ovarium merupakan kanker ginekologi yang dijumpai hampir 30% dari semua kanker organ reproduksi. Tipe paling umum dijumpai adalah kanker ovarium yang berasal dari epitel permukaan ovarium. Differensiasi menjadi jenis sel-sel epitel pada kanker ovarium jenis epitel berada pada kontrol yang sama dan nantinya akan menentukan jenis epitel pada organ sistem reproduksi wanita selama perkembangan embrionik. Protein gene product (PGP9.5) merupakan suatu peptide spesifik pada neuron yang menghilangkan ubiquitin dari protein-protein ubiquinated dan dapat berperan sebagai promoter hipermetilasi yang penting dalam gen transkripsi pada kanker. Tujuan penelitian ini untuk melihat hubungan ekspresi imunohistokimia PGP9.5 pada berbagai derajat histopatologi kanker ovarium tipe adenokarsinoma serosum dan adenokarsinoma musinosum. Metode Penelitian ini bersifat deskriptif analitik dengan pendekatan penelitian cross sectional. Penelitian ini dilakukan dengan pengambilan dan pengumpulan blok parafin jaringan ovarium sebanyak 45 sampel terdiri dari 22 sampel adenokarsinoma serosum dan 13 sampel adenokarsinoma musinosum, dipulas dengan imunohistokimia PGP9.5 dan dilakukan interpretasi dengan menilai intensitas kualitas tampilan warna pada sel tumor. Hasil Berdasarkan analisa dengan uji Kruskal-Wallis didapati 26 sampel yang terpulas negatif dengan imunohistokimia PGP9.5 terdapat 13 kasus dengan derajat differensiasi well differentiated (50%), 6 kasus (23,1%) moderately differentiated dan 7 kasus (26,9%) poorly differentiated. Pada 5 sampel yang terekspresi lemah tedapat 4 kasus (80%) dengan well differentiated dan 1 kasus (20%) dengan poorly differentiated. Pada 4 kasus terekspresi sedang, terdapat 2 kasus (50%) pada well differentiated, 1 kasus (25%) pada moderately differentiated dan 1 kasus (25%) pada poorly differentiated. Pada 10 sampel yang terekspresi kuat, terdapat 2 kasus(20%) pada well differentiated dan 8 kasus (80%) pada poorly differentiated Kesimpulan Tidak ada hubungan ekspresi PGP9.5 dengan derajat histopatologi kanker ovarium tipe adenokarsinoma serosum dan adenokarsinoma musinosum (p-value >0,05). Kata kunci: adenokarsinoma musinosum, adenokarsinoma serosum, imunohistokimia, kanker ovarium, protein gene product (PGP9.5).

Hubungan Ekspresi CD117 dan Epidermal Growth Factor Receptor (EGFR) terhadap Grading Histopatologi Adenoid Cystic Carcinoma pada Kelenjar Liur Ren Astrid Allail Siregar; T. Ibnu Alferraly; Delyuzar
Majalah Patologi Indonesia Vol. 29 No. 1, Januari 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i1.402

BackgroundTend to have adenoid cystic carcinoma (AdCC) is one of malignant epithelial tumor of the salivary gland poor prognosis, localrecurrence and metastases. Histopathological grading system according Weert, et al. observed from presence or absence solidcomponent of AdCC. CD117 is a transmembrane tyrosine kinase receptor usually strong expression in AdCC. EGFR belongs to theErbB family of receptor tyrosine kinases associated with cell proliferation, angiogenesis and metastases. Patients with advanceddisease may benefit from targeted therapy besides conventional management. Expression of CD117 and EGFR may give proposaltherapy through difference histology pattern of AdCC based on histopathological grading.MethodsThis is an analytical descriptive study with cross sectional approach. Formalin-fixed paraffin-embedded sections from 35 adenoidcystic carcinomas were obtained for histopathological grading and for immunostained of CD117 and EGFR using monoclonalantibodies. The staining intensity and percentage of cells with membranous and cytoplasmic of CD117 and EGFR expression werescored and then grouped according to the parameters of the Allred scoring system.ResultsChi-square test between CD117, EGFR expression and histopathological grading of AdCC showed no sigficant differences.Besides, McNemar test between CD117 and EGFR expression and histopathological grading AdCC showed significant differences,p-value=0.03 (p<0.05).ConclusionsCD117 and EGFR could be used to detected salivary gland AdCC

The Association between Matrix Metalloproteinase-9 (MMP-9) Immunohistochemical Expression and Gleason Grading System International Society of Urological Pathology (ISUP) in Prostate Adenocarcinoma Sefparina Harahap; Jessy Chrestella; Lidya Imelda Laksmi; T. Ibnu Alferraly; Betty Betty
Majalah Patologi Indonesia Vol. 35 No. 1, Januari 2026
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v35i1.635

Background Prostate cancer is the fourth ranked malignancy and the eighth leading mortality worldwide. The Gleason grading system is used to evaluate the tumor grade. The highest the score, the worst and the most aggressive the nature of the tumor, which associated with invasive behaviour. In prostate carcinoma, Matrix Metalloproteinase-9 (MMP-9) is involved in all stages of cancer cell progression include proliferation, angiogenesis, apoptosis, epithelial-mesenchymal transition (EMT) and metastasis. MMP-9 degrade the surrounding tumor matrix and neural tissue during perineural invasion in prostate carcinoma which is a prerequisite for invasion and metastasis. The aim of this research is to evaluate the correlation of MMP-9 immunohistochemical expression with the Gleason Grading System International Society of Urological Pathology (ISUP) of prostate adenocarcinoma. Methods Cross sectional analytic study of 32 samples were diagnosed with prostate adenocarcinoma at H. Adam Malik General Hospital Medan. Staining was performed using a polyclonal antibody rabbit MMP-9 immunohistochemistry (Bioenzy) which would appear positive in the cytoplasm and in stroma of the tumor cell at a dilution of 1: 100 (overnight). MMP-9 expression was assessed semi quantitatively based on intensity and tumor cell distribution with four-tier expression categories. Data analysis was tested with the Kruskal-Wallis test. Results Most cases (56,25%) were strongly positive with MMP-9 and MMP-9 immunohistochemical expression has a significant relationship with Gleason Grading ISUP System of prostate adenocarcinoma (p:0.001). Conclusion There was a significant association between MMP-9 immunohistochemical expression and the Gleason Grading ISUP System of prostate adenocarcinoma. Keyword: Prostate adenocarcinoma, ISUP, Gleason grading, matrix metalloproteinase-9

Relationship of the Number of Black Dots in the Nuclei of Argyrophilic Nucleolar Organizing Regions (AgNORs) Expressed Epithelial Cells with Nottingham Grading System in Invasive Breast Carcinoma of No Special Type (IBC-NST) Mohd. Yuwanda Basrul; Delyuzar Delyuzar; T. Kemala Intan; Soekimin Soekimin; T. Ibnu Alferraly
Majalah Patologi Indonesia Vol. 35 No. 1, Januari 2026
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v35i1.669

Background Breast cancer is a malignant disease in women. The high number of breast cancer cases is caused by multiple factors, including barriers to early detection of breast cancer, socio-economic and geographical factors, and the difficulty of affordable, timely and effective health services. IBC-NST has the highest incidence among breast cancer cases with a 10 year survival rate (65-78%). AgNORs parameters have been shown to represent a reliable tool for determining the clinical outcome of cancer diseases, being an independent prognostic factor in many types of tumors. Method Descriptive analytics research was carried out on slides from 31 patients with IBC-NST. Each slide was stained with H&E stainning to assess the Nottingham grading system and stained with AgNORs to assess the number of black dots in epithelial cell nuclei that expressed AgNORs. All clinicopathological data were taken from medical records/pathology archives. Logistic regression test (p<0.005) was used to assess the relationship between the number of black dots in epithelial cell nuclei expressing AgNORs and the Nottingham grading system in IBC-NST. Results The highest cases were in the age group ≤40-50 years (range: 50.1 years), the highest grading was in grade 3 of the Nottingham grading system (48.8%), the lowest number of black dots was 576 dots and the highest was 1,036 dots (mean 798.80, median 710.0 and standard deviation 144.71) and the highest number of black dots was in grade 3 of the Nottingham grading system. Conclusion Assessment of the number of black dots in the nuclei of epithelial cells expressing AgNORs associated with Nottingham grading system can be used as an indication of prognosis in patients with IBC-NST.

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