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Ace Baehaki
Jurusan Teknologi Hasil Perikanan Fakultas Pertanian Universitas Sriwijaya, Indralaya, Ogan Ilir 30662 Sumatera Selatan Telp./Fax. (0711) 580934
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Immunology & microbiology Industrial & Manufacturing Engineering Public Health Social Sciences Other

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Journal : Jurnal Perikanan Universitas Gadjah Mada

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Partial Characterization of Chitosanase from Bacillus cereus Strain BFE5400 Isolated from Snakehead Fish Intestine Shanti Dwita Lestari; Ace Baehaki; Guttifera Guttifera
Jurnal Perikanan Universitas Gadjah Mada Vol 22, No 1 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.43746

Abstract

Feeding pattern of wild snakehead fish (Channa striata) which includes shrimp and small crustaceans makes it is intestine a good source of chitinase and chitosanase enzymes. This study aimed to isolate chitosanase producing bacteria from snakehead fish intestine, to select isolate with highest chitosanolytic activity, to produce chitosanase using the selected isolate and partially characterize the resulting enzyme. I2 isolate exhibited the highest chitosanolytic index with the value of 1.5 and produced enzyme with an activity of 0.00331 U/mL after 72 hours incubation. The ultimate bacterial density was at the 24th hour with a turbidity value of 0.5315. The enzyme activity was optimum at pH 6, with a value of 0.00816 U/mL, while the optimum temperature was 70°C with the enzyme activity of 0.00785 U/mL. Genetic identification of I2 isolate using 16S rRNA showed that the isolate has a high similarity to Bacillus cereus strain BFE 5400.
Co-Authors Abd. Rasyid Syamsuri Achmad Rizky Romadhoni Agus Ramiadi Agus Supriadi Agus Supriyanto Anggraini, Amalia Anggraini, Amalia Arief - Budiman Dahlia Puspita Sari Dica Fusva Hildianti Eko Nopriansyah Elmeizy Arafah Ervianti Ervianti Erwin Saputra Firnanda Citra Gemala Cahya Guttifera Guttifera Guttifera Hafif Subarka Haidir Ali Hernandes Sitorus Pane Herpandi . Husni Mahfuz I Made Hadi Purnomo Ikbal Syukroni Imam Mahdi Rahmatullah Indah Widiastuti Indah Widiastuti Kiki Yulianti Kiki Yuliati Lisa Violita MAGGY T. SUHARTONO Ni Made Pratiwi Nugroho, Gama Dian NUNI GOFAR Nurhadi Wiranata Olima Zega Pangawikan, Aldila Din Puspa Ayu Pitayati Rachmawati, Siti Hanggita Rendy Primadona Reny Meliza Ridhowati, Sherly Rinto - - Rinto . Rinto . Rinto Rinto Rodiana Nopianti Rodiana Nopianti Rohmania, Ainur Rosalina Rosalina Selly Ratna Sari Shanti Dwita Lestari Shanti Dwita Lestari Shanti Dwita Lestari Shanti Dwita Lestari Shanti Lestari Shinta Ayu Nuryani Siti Hanggita Rachmawati J Sumantri John Toynbe Susi Lestari Susi Lestari Susi Lestari TATI NURHAYATI Titik Fadilah Amelia Yunindyawati Yunindyawati Zainona Ratnasari