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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati TROPICAL GENETICS Tropical Genetics
SONY HERU SUMARSONO
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Medicine & Pharmacology Veterinary

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Journal : HAYATI Journal of Biosciences

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Development of Domestic Cat Embryo Produced by Preserved Sperms KARTINI ERIANI; ARIEF BOEDIONO; ITA DJUWITA; SONY HERU SUMARSONO; AL-AZHAR AL-AZHAR
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.643 KB) | DOI: 10.4308/hjb.15.4.155

Abstract

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization
Co-Authors AL-AZHAR AL-AZHAR Arief Boediono Bambang Suryobroto Eka Pasana Pujowati Indra Wibowo Ayda T Yusuf Ita Djuwita KARTINI ERIANI MAELITA RAMDANI MOEIS MAELITA RAMDANI MOEIS Rini Widyastuti Siti Darodjah Rasad WOLLY CANDRAMILA