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Development of Domestic Cat Embryo Produced by Preserved Sperms KARTINI ERIANI; ARIEF BOEDIONO; ITA DJUWITA; SONY HERU SUMARSONO; AL-AZHAR AL-AZHAR
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization

Evaluasi Kualitas Spermatozoa Pada Beberapa Frekuensi Ejakulasi Terhadap Kerbau Lokal (Bubalus bubalis) Rosnizar Rosnizar; Nurfajri Nurfajri; Dasrul Dasrul; Amalia Amalia; Kartini Eriani
Jurnal Bioleuser Vol 5, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, University of Syiah Ku

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/j. bioleuser.v5i1.22975

Faktor frekuensi ejakulasi merupakan salah satu faktor penting yang mempengaruhi kualitas spermatozoa semen segar. Penelitian ini bertujuan untuk menentukan frekuensi ejakulasi yang terbaik terhadap kualitas spermatozoa kerbau lokal. Rancangan penelitian adalah rancangan acak lengkap (RAL) dengan tiga perlakuan (ejakulat pertama, ejakulat kedua dan ejakulat ketiga) Hasil penelitian menunjukkan bahwa frekuensi ejakulasi berpengaruh terhadap volume, persentase motilitas, persentase spermatozoa hidup dan konsentrasi spermatozoa semen segar, namun tidak berpengaruh terhadap abnormalitas spermatozoa. Volume, persentase motilitas dan konsentrasi spermatozoa semen segar paling tinggi ditemukan pada ejakulat pertama sedangkan persentase spermatozoa hidup paling tinggi ditemukan pada ejakulat kedua dan menunjukkan bahwa kerbau lokal layak digunakan untuk inseminasi buatan namun ejakulasi pertama dan ejakulasi kedua cenderung lebih baik dari ejakulasi ketiga.

Morphology and Parasitaemia Development of Plasmodium berghei in Balb/c Mice (Mus musculus) Rosnizar Rosnizar; Kartini Eriani
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1372.055 KB)

Malaria is one of the most severe public health problems worldwide. It is a leading cause of death and disease in many developing countries, where young children and pregnant women are the groups most affected. Malaria disease caused by Plasmodium parasite have symptoms that typically include fever, fatigue, vomiting and headaches. In severe cases, it can cause yellow skin, seizures, coma or death. The present study is aimed to monitoring parasitemia level and percentage of parasite morphology as parasitaemia progresses. This research used Plasmodium berghei NK strain obtained from National University of Malaysia which originally from MR4, USA. Design used in this research was completely randomized design, with 2 treatments which were mice without infection and mice with infection of P. berghei parasite. Method used in this research was staining method of thin smear of blood using Giemsa stained and observed by microscopic. Parameter of observation were morphology and development of P. berghei at different level of parasitaemia (10%, 20%, 30% and 40%). The result showed from microscopic examination of blood slides prepared from the study animals indicated that the ring form was dominant stage obtained at all different stages of infection followed by trophozoite stage. Meanwhile schizont stage was the lowest stage obtained at all different stages observed.

Pengaruh suhu terhadap perkembangan embrio ikan Cupang Betta splendens Mustaqim Mustaqim; Kartini Eriani; Erlangga Erlangga; Rachmawati Rusyidi
Depik Vol 8, No 3 (2019): December 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak. Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis

A short review of discovery and development of fish sperm cryopreservation Dian Afriani; Kartini Eriani; Zainal Abidin Muchlisin; Iwan Hasri
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Global biodiversity, especially fish, has experienced a decline, this occurs as a result of over-exploitation, the presence of introduced fish species and climate change. This condition makes researchers look for solutions to overcome these problems by using cryopreservation techniques. The main purpose of cryopreservation is to store, maintain, and ensure the survival of genetic material, so that using cryopreservation techniques can maintain the viability and function of gamete cells both immunologically, biologically and physiologically. The success of the cryopreservation technique has made this technique widely developed in various species of living organism including fish. This article summarizes and reviews the history of the development of cryopreservation of animal species with specific focus on fish.Keywords:CryopreservationHistoryDepikEndemic species

Pengaruh suhu terhadap perkembangan embrio ikan Cupang Betta splendens Mustaqim Mustaqim; Kartini Eriani; Erlangga Erlangga; Rachmawati Rusyidi
Depik Vol 8, No 3 (2019): December 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.8.3.13916

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak. Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis

A short review of discovery and development of fish sperm cryopreservation Dian Afriani; Kartini Eriani; Zainal Abidin Muchlisin; Iwan Hasri
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.1.18794

Global biodiversity, especially fish, has experienced a decline, this occurs as a result of over-exploitation, the presence of introduced fish species and climate change. This condition makes researchers look for solutions to overcome these problems by using cryopreservation techniques. The main purpose of cryopreservation is to store, maintain, and ensure the survival of genetic material, so that using cryopreservation techniques can maintain the viability and function of gamete cells both immunologically, biologically and physiologically. The success of the cryopreservation technique has made this technique widely developed in various species of living organism including fish. This article summarizes and reviews the history of the development of cryopreservation of animal species with specific focus on fish.Keywords:CryopreservationHistoryDepikEndemic species

A short review of discovery and development of fish sperm cryopreservation Dian Afriani; Kartini Eriani; Zainal Abidin Muchlisin; Iwan Hasri
Depik Vol 10, No 1 (2021): April 2021
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.10.1.18794

Global biodiversity, especially fish, has experienced a decline, this occurs as a result of over-exploitation, the presence of introduced fish species and climate change. This condition makes researchers look for solutions to overcome these problems by using cryopreservation techniques. The main purpose of cryopreservation is to store, maintain, and ensure the survival of genetic material, so that using cryopreservation techniques can maintain the viability and function of gamete cells both immunologically, biologically and physiologically. The success of the cryopreservation technique has made this technique widely developed in various species of living organism including fish. This article summarizes and reviews the history of the development of cryopreservation of animal species with specific focus on fish.Keywords:CryopreservationHistoryDepikEndemic species

Pengaruh suhu terhadap perkembangan embrio ikan Cupang Betta splendens Mustaqim Mustaqim; Kartini Eriani; Erlangga Erlangga; Rachmawati Rusyidi
Depik Vol 8, No 3 (2019): December 2019
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.8.3.13916

Abstract. Temperurate is one of the important parameter in the embryo development of fish; However, to date no information this effect on the Betta splendens. Hence, this study aims to determine the effect of temperature on egg hatchability and survival of betta fish larvae. Samples of betta fish used in this study are similar in size and from the same type of broodstock. A completely randomized design used with four treatments and three replications. The parameters observed were the process of egg development, fertilization rate, hatching rate, hatching duration, survival rate, and water quality (pH, DO, temperature). The results showed that the relation between temperature and fertilization rate was not significantly different, whereas the calculated F value (0.23) ˂ F table 0.05 (4.07). The relation between temperature and hatching rate has a very significant effect, F value (18.91) ˃ F table 0.05 (4.07). The relation of temperature to hatching time showed the same difference, and the relation of temperature to survival of betta fish (Betta spelendes) larvae is significant with F ¬ values (39.98) F table 0.05 (4.07). Water quality parameters during the study were pH 7 - 7.5, DO 5.0 - 7.7 ppm and temperature at 27 - 29 oC.Keywords: Betta fish eggs, temperature, embryogenesis Abstrak. Suhu adalah salah satu faktor menentukak proses perkembangan embrio pada ikan, namun pengaruh ini belum pernah diamati pada ikan cupang Betta splendens. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh suhu terhadap perkembangan embrio, daya tetas telur dan kelangsungan hidup larva ikan cupang. Induk ikan cupang yang digunakan memiliki ukuran yang sama dan dari induk jenis yang sama. Adapun rancangan yang digunakan dalam penelitian adalah rancangan acak non factorialdengan 4 perlakuan 3 kali ulangan. Parameter yang diamati adalah proses perkembangan telur, tingkat pembuahan telur, tingkat penetasan telur, lama penetasan telur, tingkat kelangsungan hidup dan kualitas air pH, DO, suhu ruangan. Hasil penelitian menunjukkan bahwa hubungan suhu terhadap tingkat pembuahan telur tidak berbedanyata, dimana nilai F hitung (0,23) ˂ F tabel 0,05 (4,07). Hubungan suhu terhadap tingkat penetasan telur berpengaruh sangat berbedanyata, dimana nilai F hitung (18,91) ˃ F tabel 0,05 (4,07). Hubungan suhu terhadap lama penetasan menunjukkan berbeda sama, dan hubungan suhu terhadap kelangsungan hidup berpengaruh nyata terhadap kelangsungan hidup larva ikan cupang (Betta spelendes) dengan nilai F hitung (39,98) F tabel 0,05 (4,07). Parameter kualitas air selama penelitian rata-rata pH 7 – 7,5, DO 5,0 – 7,7 ppm dan suhu ruangan 27 – 29 oC.Kata kunci: Telur ikan cupang, suhu, embriogenesis

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